The Development and Validation of a Measure of Identity Distress

The most frequently used measures of identity development do not contain a scale to measure the distress that can sometimes be associated with the process. The purpose of this study was to assess the reliability and validity of the Identity Distress Survey. The measure was found to have high internal consistency and test–retest reliability. Identity distress was related to the constructs of identity style and identity status. Identity distress was positively correlated with identity exploration and associated with the informational style and was negatively correlated with identity commitment. This measure could help identify those people who are having exceptional difficulties in the process of identity development and in need of intervention

Iron deposition is independent of cellular inflammation in a cerebral model of multiple sclerosis

<p>Abstract</p> <p>Background</p> <p>Perivenular inflammation is a common early pathological feature in multiple sclerosis (MS). A recent hypothesis stated that CNS inflammation is induced by perivenular iron deposits that occur in response to altered blood flow in MS subjects. In order to evaluate this hypothesis, an animal model was developed, called cerebral experimental autoimmune encephalomyelitis (cEAE), which presents with CNS perivascular iron deposits. This model was used to investigate the relationship of iron deposition to inflammation.</p> <p>Methods</p> <p>In order to generate cEAE, mice were given an encephalitogen injection followed by a stereotactic intracerebral injection of TNF-α and IFN-γ. Control animals received encephalitogen followed by an intracerebral injection of saline, or no encephalitogen plus an intracerebral injection of saline or cytokines. Laser Doppler was used to measure cerebral blood flow. MRI and iron histochemistry were used to localize iron deposits. Additional histological procedures were used to localize inflammatory cell infiltrates, microgliosis and astrogliosis.</p> <p>Results</p> <p>Doppler analysis revealed that cEAE mice had a reduction in cerebral blood flow compared to controls. MRI revealed T2 hypointense areas in cEAE animals that spatially correlated with iron deposition around vessels and at some sites of inflammation as detected by iron histochemistry. Vessels with associated iron deposits were distributed across both hemispheres. Mice with cEAE had more iron-labeled vessels compared to controls, but these vessels were not commonly associated with inflammatory cell infiltrates. Some iron-laden vessels had associated microgliosis that was above the background microglial response, and iron deposits were observed within reactive microglia. Vessels with associated astrogliosis were more commonly observed without colocalization of iron deposits.</p> <p>Conclusion</p> <p>The findings indicate that iron deposition around vessels can occur independently of inflammation providing evidence against the hypothesis that iron deposits account for inflammatory cell infiltrates observed in MS.</p

Computational identification of developmental enhancers: conservation and function of transcription factor binding-site clusters in Drosophila melanogaster and Drosophila pseudoobscura

BACKGROUND: The identification of sequences that control transcription in metazoans is a major goal of genome analysis. In a previous study, we demonstrated that searching for clusters of predicted transcription factor binding sites could discover active regulatory sequences, and identified 37 regions of the Drosophila melanogaster genome with high densities of predicted binding sites for five transcription factors involved in anterior-posterior embryonic patterning. Nine of these clusters overlapped known enhancers. Here, we report the results of in vivo functional analysis of 27 remaining clusters. RESULTS: We generated transgenic flies carrying each cluster attached to a basal promoter and reporter gene, and assayed embryos for reporter gene expression. Six clusters are enhancers of adjacent genes: giant, fushi tarazu, odd-skipped, nubbin, squeeze and pdm2; three drive expression in patterns unrelated to those of neighboring genes; the remaining 18 do not appear to have enhancer activity. We used the Drosophila pseudoobscura genome to compare patterns of evolution in and around the 15 positive and 18 false-positive predictions. Although conservation of primary sequence cannot distinguish true from false positives, conservation of binding-site clustering accurately discriminates functional binding-site clusters from those with no function. We incorporated conservation of binding-site clustering into a new genome-wide enhancer screen, and predict several hundred new regulatory sequences, including 85 adjacent to genes with embryonic patterns. CONCLUSIONS: Measuring conservation of sequence features closely linked to function - such as binding-site clustering - makes better use of comparative sequence data than commonly used methods that examine only sequence identity

Promoting Positive Identity Development in Troubled Youth: A Developmental Intervention Science Outreach Research Approach

This article illustrates how developmental intervention science outreach research contributes to knowledge development on the promotion of positive identity development by describing results from the Miami Youth Development Project. The project is committed to the use of descriptive and explanatory knowledge about evidence-based individual and institutional intervention strategies for promoting developmental change in self and identity. Our efforts, described here, include a method for measuring theoretically and personally meaningful identity change, a procedure for integrating key aspects of qualitative and quantitative data through relational data analysis, and an evidence-based positive youth development intervention that fosters measurable and meaningful identity change

A Developmental Intervention Science (DIS) outreach research approach to promoting youth development: Theoretical, methodological, and meta-theoretical challenges.

This paper describes work directed toward creating community-supported positive youth development interventions that draw on a developmental intervention science outreach research approach. With respect to developmental interventions, this approach focuses on creating evidence-based longitudinal change intervention strategies for promoting long-term developmental change. The paper describes three broad challenges (theoretical, methodological, and meta-theoretical) that the authors faced in their efforts to develop and implement community-supported intervention programs built on this approach. The authors describe first the theoretical challenges they addressed in developing the conceptual framework for their community-supported intervention; second, the challenge of developing and refining a methodological framework for evaluating positive youth development interventions in “real-world” settings; and third, the meta-theoretical challenges that arose in the context of implementing community-supported positive development programs

A Developmental Intervention Approach to Promoting Positive Development: Pathways of Intervention Change

This paper describes work directed toward creating community-supported positive youth development interventions that draw on a developmental intervention science outreach research approach. With respect to developmental interventions, this approach focuses on creating evidence-based longitudinal change intervention strategies for promoting long-term developmental change. The paper describes three broad challenges (theoretical, methodological, and meta-theoretical) that the authors faced in their efforts to develop and implement community-supported intervention programs built on this approach. The authors describe first the theoretical challenges they addressed in developing the conceptual framework for their community-supported intervention; second, the challenge of developing and refining a methodological framework for evaluating positive youth development interventions in “real-world” settings; and third, the meta-theoretical challenges that arose in the context of implementing community-supported positive development programs

Promoting Positive Youth Development The Miami Youth Development Project (YDP)

The Miami Youth Development Project (YDP) had its beginnings in the early 1990s as a grassroots response to the needs of troubled (multiproblem) young people in the community (Arnett, Kurtines, & Montgomery, 2008, this issue). YDP is an important outcome of efforts to create positive youth development interventions that draw on the strengths of developmental intervention science outreach research in the development of community-supported positive development programs (i.e., an approach that focuses on meeting community needs as well as youth needs by generating innovative knowledge of evidence-based change intervention strategies that are feasible, affordable, and sustainable in “real world” settings, (Kurtines, Ferrer-Wreder, Cass Lorente, Silverman, Montgomery, 2008, this issue). Now completing its second decade, YDP represents an effort to bring together a more empowering model of knowledge development for research involvement in the community, a nuanced and contextualized notion of youth and their development, and methodologies that richly reflect rather than reduce the experiences of the young people whose development the authors seek to promote

Performance of the double multilayer monochromator on the NSLS wiggler beam line X25

A tunable, double multilayer x-ray monochromator has recently been implemented on the National Synchrotron Light Source (NSLS) X25 wiggler beam line. It is based on a parallel pair of tungsten-boron-carbide multilayer films grown on silicon substrates and purchased from Osmic, Inc. of Troy, Michigan, USA. It acts as an optional alternative to the conventional double silicon crystal monochromator, and uses the same alignment mechanism. Two other NSLS beam lines also have had this kind of monochromator installed recently, following the lead of the NSLS X20C IBM/MIT beam line which has used a double multilayer monochromator for several years. Owing to the 100 times broader bandwidth of a multilayer x-ray monochromator, compared with a silicon monochromator, the multilayer monochromator has the obvious advantage of delivering 100 times the flux of a silicon monochromator, and thereby makes more efficient use of the continuous synchrotron radiation spectrum, yet preserves the narrow collimation of the incident synchrotron beam. In particular, multilayer x-ray bandwidths, on the order of 1%, are well-matched to x-ray undulator linewidths. Performance results for the X25 multilayer monochromator are presented, comparing it with the silicon monochromator. Of note is its short- and long-term performance as an x-ray monochromator delivering the brightness of the wiggler source in the presence of the high-power white beam. Detailed measurements of its spatial beam profile and wavelength dispersion have been made, and it is shown how its resolution could be improved when desired. Finally, its peculiar, anisotropic resolution function in reciprocal space, and its bearing upon x-ray crystallography and scattering experiments, will be discussed, and highlighted by the results of a protein crystallography experiment. © 1997 American Institute of Physics.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/87844/2/71_1.pd