42 research outputs found
PrtT-Regulated Proteins Secreted by Aspergillus fumigatus Activate MAPK Signaling in Exposed A549 Lung Cells Leading to Necrotic Cell Death
Get PDFAspergillus fumigatus is the most commonly encountered mold pathogen of humans, predominantly infecting the respiratory system. Colonization and penetration of the lung alveolar epithelium is a key but poorly understood step in the infection process. This study focused on identifying the transcriptional and cell-signaling responses activated in A549 alveolar carcinoma cells incubated in the presence of A. fumigatus wild-type and ΞPrtT protease-deficient germinating conidia and culture filtrates (CF). Microarray analysis of exposed A549 cells identified distinct classes of genes whose expression is altered in the presence of germinating conidia and CF and suggested the involvement of both NFkB and MAPK signaling pathways in mediating the cellular response. Phosphoprotein analysis of A549 cells confirmed that JNK and ERK1/2 are phosphorylated in response to CF from wild-type A. fumigatus and not phosphorylated in response to CF from the ΞPrtT protease-deficient strain. Inhibition of JNK or ERK1/2 kinase activity substantially decreased CF-induced cell damage, including cell peeling, actin-cytoskeleton damage, and reduction in metabolic activity and necrotic death. These results suggest that inhibition of MAPK-mediated host responses to treatment with A. fumigatus CF decreases cellular damage, a finding with possible clinical implications
Permanent Collection 026
No full textThis piece, created by Haim Steinbach (titled 00:07 3, 5L, and the 7th in series), was created in 1988 and consists of 3 cans and 5 lights, long version, measuring 66 x 122 x 21 . It is part of the 1989 permanent collection in the Robert and Elain Stein Galleries located at Wright State University.https://corescholar.libraries.wright.edu/restein_permanent/1025/thumbnail.jp
Redefining the Object 016: End of Day Obsession (2)
No full textReDefining the Object, exhibition held at the Robert and Elaine Stein galleries at Wright State University from February 22 through April 3, 1988. The piece shown in this image was created in 1987 by Haim Steinbach and is titled End of Day Obsession (2) . It was created using black medicine balls, Calphalon stock pots, black towels, gun lites , and a wood laminate shelf. It measures 137 x 40.25 x 17.5 .https://corescholar.libraries.wright.edu/restein_object/1015/thumbnail.jp
