Additional file 3: of Early treatment with hydroxychloroquine prevents the development of endothelial dysfunction in a murine model of systemic lupus erythematosus

Relaxation associated with acetylcholine in basal conditions (saline) or in the presence of l -NAME, diphenyleneiodinium (DPI) or both in mesenteric arteries from NZ animals at baseline and at different time points. Each point represents the mean of six experiments ± SEM. *P < 0.001; † P < 0.01; ‡ P < 0.05. (PPT 246 kb

Adhesion genes and SNPs selected for association study with RA.

<p>* Linkage regions according to Osorio et al. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103872#pone.0103872-Davis1" target="_blank">[5]</a>.</p><p>LAD = leukocyte adhesion defect; SLE = systemic lupus erythematodes; DM = diabetes mellitus; AIH = autoimmune hepatitis; CP = cardiopathy.</p

Possible losses or gains of transcription factor binding sites for rs6136 and rs9332575.

<p>Prediction of altered transcription factor binding sites due to alleles of rs6136 and rs9332575. rs9332575 is a neighboring variant for rs6136 which is in perfect linkage disequilibrium. Core sim.: score for highest conserved nucleotides of a known TFBS. Matrix sim.: score for highest conserved nucleotides of a known TFBS family matrix. Similarity scores >0.8 indicate relevant predictions <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0103872#pone.0103872-Cartharius1" target="_blank">[38]</a>.</p

Results of genetic association analysis for rs6136 – <i>SELP</i> (minor allele G).

<p>Shown are results in all samples in a given sample set; Minor allele transmissions (transmitted alleles/untransmitted alleles) are shown. Informative parents: Number of parental individuals carrying a heterozygous genotype TDT: Transmission disequilibrium test considering informative parents, only. OR (CI) allelic odds ratio and 95% confidence interval of the minor allele when considering all parents (transmitted vs. non-transmitted alleles). Minor allele frequency relates to controls (non-transmitted alleles).</p

Cis-directed DAE pattern of rs6136 in the <i>SELP</i> gene.

<p>Green boxplots indicate samples resulting from allele specific expression analysis of cDNA. Samples tagged “RA” were derived from RA patients, which was not the case for samples 1–4; gDNA = allelic ratios of gDNA samples which served as reference. SNR = signal to noise ratio. Stars indicate cDNA samples with increased expression of transcripts carrying variant rs6136-T. ** = p-val<0.01, *** = p-value<0.001.</p

<i>SELP</i> expression profiles for human RA compared to healthy tissue and pristane induced arthritis in rat.

<p>Data derived from Gene Expression Atlas (E-GEOD-7307). N_normal = 679 (including healthy joint synovium and synovial membrane samples), N_RA = 5, N_{joint synovium} = 3 (all healthy), N_{synovial membrane} = 11 (N_normal = 6, N_RA = 5). N_{rats-untreated} = 5, N_{rats+pristane} = 5.</p

Characteristics of rheumatoid arthritis (RA) index cases from the investigated samples.

<p>N = number of families; RF⁺ = rheumatoid factor positive.</p