Reproducibility and dose dependency of the antitumoral pharmacodynamic effect of some autochthonous polysaccharidic or polyphenolic biopreparations of fungal and vegetal origin

The testing of the yeast Candida inconspicua in the decolorization process of waste coloured water, resulted from the dye process of the textile fiber, revealed the presence of two possible mechanisms by azoic - dyes are removing from effluents, as follow: adsorbtion, correlated with a low azoreductase activity, in the case of Acid Blue 113 and biodegradation, as a result of reductive activity of azoreductase towards diazo = bonds, in the case of Basic Blue 41. The experiment revealed also, the dependence of enzymatic activity by temperature

Dynamics of alkaloid biosynthesis in correlation with lipid biosynthesis in submerged cultivated strains of Claviceps purpurea

Lipid metabolism is associated with alkaloid biosynthesis due to acetyl-CoA common precursor. For this reason, in the present paper the dynamics of total alkaloid content is investigated both in mycelia and supernatants of some submerged cultivated strains of Claviceps purpurea, as well as the dynamics of the brute lipid amount in the mycelia of the studied strains. The comparative analysis of the results concerning the total alkaloid content in the mycelia of analyzed strains and in the supernatants of culture media generally shows that the profile of the variation curves is comparable for the analyzed strains and the value amplitudes are dependent of them. At certain ages, as a main trend we found that the lipid accumulation in the mycelia of investigated strains takes place in a parallel manner with alkaloid biosynthesis and the crude lipid level is kept stable or diminishes after alkaloid biosynthesis begins

Preliminary studies concerning chromosome constitution of some Cornus mas L. genotypes (Bacau district)

: The cytogenetic characterization is necessary to decipher the controversies on biogeography, taxonomy and evolution of the genus, evolution of chromosome number. Some of Cornus species have the following diploid chromosome numbers: Cornus suecica L. 2n=22, Cornus mas L. 2n=18, 54, Cornus sanguinea L. 2n=22, Cornus sericea L. 2n=22, Cornus alba 2n=22. Our preliminary observations performed on Cornus mas genotypes (Bacau district) confirmed the existence of 2n=18 chromosomes. Karyotypic formula is 2n = 18 = 14m + 4sm, the centromeres having median and submedian position. Chromosome size ranged between 2.92 4.63 microns, with variability limits from 2.86 to 4.71 microns. The length of haploid complement is 33.62 microns. Centromeric index varies from 36.14 to 44.83, while the arm ratio values are comprised in interval 1.23 - 1.77

IN VITRO SELECTION OF SOME POTENTIAL CYTOSTATIC AGENTS FROM NEW FUNGAL EXOPOLYGLUCIDIC EXTRACTS

The in vitro action of some new autochthonous glucanic biopreparations, specifically extracted from diverse submerged strains of Claviceps purpurea, upon the proteinsynthesis of Hep-2p cancerous cells as well as upon the development of the corresponding cellular cultures was investigated. The significant perturbation of proteic biogenesis, the modification of the protein dynamics, the inhibition of the cell cultures development and the existence of a doseresponse relationship argue the behaviour of the low intensity and frequency electromagnetic field as in vitro active cytostatic agent. This primary characterization of some glucanic extracts as cytostatic agent justifies the study of their effect upon cell proliferation and viability in order to enlarge the reasoning basis for the introduction of those exopolysaccharidic agents in the in vivo antitumoral screening program on different experimental tumoral systems

The cytostatic potential confirmation of some fungal autochthonous biopreparations upon HeLa neoplastic cells cultures

Some biopreparations of alkaloid-ergolinic nature have been extracted from hyphal and supernatant components, which were centrifugally separated from the submerged culture media of three strains of Claviceps purpurea (T1-3, T2-1 and T13-1), these being in different ontogenetic development stages (4, 6, 8, 10, and 12 days, respectively). In vitro testing of their interaction with cellular protein synthesis process of HeLa neoplastic cells cultures, has highlighted the cellular protein biosynthesis alteration, modifications of the protein dynamics sense and amplitude, as well as the cell cultures development inhibition. The protein synthesis inhibitory impact has confirmed the cytostatic action of these natural bioproducts, their cytostatic effectiveness being dependent of the Claviceps purpurea (T1-3, T2-1 and T13-1) strains specificity, the strain ontogenetic age, the biochemical nature of the intracellularly synthesized, stocked and extracellularly discharged substratum, as well as of their obtaining sources

Estimation of roundup action on genetic material of Trigonella foenum graecum L

In the present study, the cytogenetic effects of the herbicide roundup on division of root meristematic cells of Trigonella foenum graecum and influence of this treatment on height plants in early ontogenetic phase were studied. The seeds of plants were treated with various concentrations of roundup (0.1%, 0.5%, 1.0 %, and 2.0 %), for 3 and 6 h. Roundup had an inhibitory effect on plantlets height in early ontogenetic phases at all tested concentrations in 6 h treatment, comparatively with control, and at maximum tested concentration applied for 3 h. The mitotic index was significantly reduced by roundup 6h, for all tested concentrations. Roundup 3h, 0.1% and 1.0% variants, and roundup 6h, 0.5% and 2.0% variants induced the most numerous ana-telophase chromosomal aberrations. The presence of micronuclei and fragments suggests the potential clastogen effect of roundup

Study regarding the adherence of oral microbiota on composit plating materials

The paper set to carry out a study about the behavior of the 3 types of composite materials (Solidex, Ceramage, Gradia) in the oral environment, by investigating the relationship between bacterial micro-flora and marginal periodontal tissue (assessing the degree of microorganisms adherence on the surface of these plating materials). Results thus obtained showed a significant difference between the two groups: study group (teeth reconstructed with composite materials) and control group (natural teeth). Although prosthetic parts were finished and polished to allow a smooth self-cleaning surface and subjects have used additional methods of oral hygiene (dental thread and mouthwash), the prosthetic surface presented a far greater number of microorganisms compared with the surface of natural counterparts teeth thus, in general, values were ten times higher in favor of the prosthetic surface compared with the natural control teet