Preliminary results on the assessment of Danube River fish species migration behavior in relation to Iron Gate I and II dam using acoustic telemetry equipment

The dam’s construction without fish passes had a major impact, obstructing migratory fish populations from moving between different parts of the river systems and utilizing their key habitats. Fish species and fish populations are depending on long migrations are most seriously affected by river fragmentation. Building well-functioning fish passage systems for migratory fish species at Iron Gate I (rkm 943) and Iron Gate II (rkm 863), is crucial for strengthening and re-establishing Danube’s River migratory fish populations. Between March and May 2021, 112 fish specimens were captured downstream Iron Gate II dam. All the fish was previously tagged with 69 KHz ultrasonic transmitters and released upstream Iron Gate II dam reservoir (53 fish) and downstream Iron Gate II dam (59 fish). Some of the acoustic transmitters are equipped with depth sensors, which provide information of water depths used by fish. Fish movements were recorded by a network of 18 ultrasonic automatic Vemco VR2w receivers (10 downstream the Iron Gate II dam and Gogosu branch, 6 in the IG II lake and 2 receivers at the Iron Gate I dam) with a 15 month battery life, positioned in Danube River. Downloading and interpreting recorded data by these receivers will be an important starting point for "We Pass 2" project that will begin in fall of 2021, when will be realized high resolution recordings of 3D fish movements, for finding the best technical solution for their passing upstream or downstream of dams

Mittelstandsvertraeglichkeit der EG-Forschungsfoerderung?

Available from Bibliothek des Instituts fuer Weltwirtschaft, ZBW, Duesternbrook Weg 120, D-24105 Kiel / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEDEGerman

Karyometry of melanocytic lesions: quantitative assessment of the 'maturation to the depth'

40 cases each of malignant melanoma, Spitz's nevus and benign intradermal nevus were examined using an interactive image analysis system. 60 consecutive nuclei were evaluated in the upper and lower portion of the melanocytic lesions. Besides basic karyometric data, a 'maturation parameter' (MP) expressing the previously discribed 'maturation to the depth' was assessed in each individual case, by calculating the ratio of the nuclear area in the deep portion and in the superficial portion. When the three parameters (superficial nuclear area, deep nuclear area and maturation parameter) were evaluated separately (k-nearest neighbour method), the efficiency of the superficial nuclear area was only 19%, compared with 72% for the deep nuclear area and 94% for the maturation parameter. Combination of the maturation parameter and the deep nuclear area provided an efficiency of 98% with a sensitivity of 98% and a specificity of 97%. The results indicate that the maturation parameter is superior to conventional karyometric data in the differentiation between benign and malignant melanocytic lesions

No Detection of Borrelia Burgdorferi-Specific Dna in Erythema Migrans Lesions After Minocycline Treatment

Background and Design: Early treatment of erythema migrans is important to prevent late complications. Minocycline possesses several attributes, making it potentially useful in the treatment of borrelial infections. In our study, minocycline was administered to 14 patients with erythema migrans. Punch biopsy specimens were obtained from the (affected) skin of all patients before and after therapy. The formalin-fixed, paraffin-embedded specimens were analyzed by polymerase chain reaction for the presence of Borrelia burgdorferi-specific DNA

Quantitative-evaluation of melanoma cell invasion in 3-dimensional confrontation cultures in vitro using automated image-analysis

Tumor invasion is a crucial feature of tumor growth in vivo. Confrontation cultures of multicellular melanoma spheroids and embryonic chick heart fragments provide a model for invasive growth in vitro. We have developed an image analysis method, which facilitates the objective measurement of tumor cell invasion in this model. Cryostat sections of confrontation cultures were immunohistochemically stained with an antiserum directed against the stromal component for automated recognition of the stroma tissue. The slides were automatically processed by a grey level based computerized image analysis system. On Spearman's rank correlation test, 25 out of 39 parameters correlated with the reference value of invasion, which was derived from the subjective evaluation of five independent observers. Two parameters combining the stroma margin and the total amount of stroma tissue completely reproduced the judgement of the morphologists in our test set. The quantitative evaluation of tumor invasion in vitro by automated image analysis may be helpful in pharmacologic and pathogenetic studies of tumor growth

Take time to smell the frogs: vocal sac glands of reed frogs (Anura: Hyperoliidae) contain species-specific chemical cocktails

Males of all reed frog species (Anura: Hyperoliidae) have a prominent, often colourful, gular patch on their vocal sac, which is particularly conspicuous once the vocal sac is inflated. Although the presence, shape, and form of the gular patch are well-known diagnostic characters for these frogs, its function remains unknown. By integrating biochemical and histological methods, we found strong evidence that the gular patch is a gland producing volatile compounds, which might be emitted while calling. Volatile compounds were confirmed by gas chromatography-mass spectrometry in the gular glands in 11 species of the hyperoliid genera Afrixalus, Heterixalus, Hyperolius, and Phlyctimantis. Comparing the gular gland contents of 17 specimens of four sympatric Hyperolius species yielded a large variety of 65 compounds in species-specific combinations. We suggest that reed frogs might use a complex combination of at least acoustic and chemical signals in species recognition and mate choice.(c) 2013 The Authors. Biological Journal of the Linnean Society published by John Wiley & Sons Ltd on behalf of The Linnean Society of London