Anti-citrullinated fibronectin antibodies in rheumatoid arthritis are associated with human leukocyte antigen-drb1 shared epitope alleles

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Anti-Mup44 autoantibodies in inclusion-body myositis

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Peptidylarginine deiminase expression and activity in pad2 knock-out and pad4-low mice

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The rheumatoid arthritis synovial fluid citrullinome reveals novel citrullinated epitopes in apolipoprotein E, myeloid nuclear differentiation antigen, and ss-actin

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The human peptidylarginine deiminases type 2 and type 4 have distinct substrate specificities

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Anti-cyclic citrullinated peptide positivity in non-rheumatoid arthritis disease samples: citrulline-dependent or not?

Contains fulltext : 35130.pdf (publisher's version ) (Closed access)BACKGROUND: Antibodies directed against citrullinated proteins (eg anti-cyclic citrullinated peptide (CCP)) have excellent diagnostic and good prognostic potential for rheumatoid arthritis. Type 1 autoimmune hepatitis (AIH-1) is a chronic liver disease characterised by a variety of serum autoantibodies. Recently, in a large group of patients with AIH-1 without clear rheumatoid arthritis overlap, a relatively high percentage (9%) of anti-CCP2 positivity was scored. OBJECTIVES: To characterise the citrulline-dependence of the observed anti-CCP2 positivity in AIH-1 sera as well as in other groups of patients without rheumatoid arthritis (mainly rheumatic diseases). METHODS: Serum samples of 57 patients with AIH-1 and 66 patients without rheumatoid arthritis, most of them reported as anti-CCP positive, were tested for citrulline-specific reactivity with a second generation anti-CCP kit, with the citrullinated and the corresponding non-citrullinated (arginine-containing) antigen. A subset of AIH-1 sera was also tested with a CCP1 ELISA (and arginine control). RESULTS: The anti-CCP2 reactivity of most non-rheumatoid arthritis rheumatic diseases samples (87-93%) was citrulline-specific, whereas a relatively high percentage of AIH-1 samples (42-50%) turned out to be reactive in a citrulline-independent manner. The use of citrullinated and non-citrullinated CCP1 peptides confirmed a high occurrence of citrulline-independent reactivity in AIH-1 samples. CONCLUSIONS: In rheumatoid arthritis and most non-rheumatoid arthritis rheumatologic disease sera, anti-CCP positivity is citrulline-dependent. However in some patients, particularly patients with AIH-1, citrulline-independent reactivity in the anti-CCP2 test can occur. A positive CCP test in a non-rheumatic disease (eg liver disease) should therefore be interpreted with care, and preferably followed by a control ELISA with a non-citrullinated antigen

Anti-CCP2 positivity in non-ra disease samples: citrulline-dependent or not?

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Anti‐cyclic citrullinated peptide positivity in non‐rheumatoid arthritis disease samples: citrulline‐dependent or not?

BACKGROUND: Antibodies directed against citrullinated proteins (eg anti‐cyclic citrullinated peptide (CCP)) have excellent diagnostic and good prognostic potential for rheumatoid arthritis. Type 1 autoimmune hepatitis (AIH‐1) is a chronic liver disease characterised by a variety of serum autoantibodies. Recently, in a large group of patients with AIH‐1 without clear rheumatoid arthritis overlap, a relatively high percentage (9%) of anti‐CCP2 positivity was scored. OBJECTIVES: To characterise the citrulline‐dependence of the observed anti‐CCP2 positivity in AIH‐1 sera as well as in other groups of patients without rheumatoid arthritis (mainly rheumatic diseases). METHODS: Serum samples of 57 patients with AIH‐1 and 66 patients without rheumatoid arthritis, most of them reported as anti‐CCP positive, were tested for citrulline‐specific reactivity with a second generation anti‐CCP kit, with the citrullinated and the corresponding non‐citrullinated (arginine‐containing) antigen. A subset of AIH‐1 sera was also tested with a CCP1 ELISA (and arginine control). RESULTS: The anti‐CCP2 reactivity of most non‐rheumatoid arthritis rheumatic diseases samples (87–93%) was citrulline‐specific, whereas a relatively high percentage of AIH‐1 samples (42–50%) turned out to be reactive in a citrulline‐independent manner. The use of citrullinated and non‐citrullinated CCP1 peptides confirmed a high occurrence of citrulline‐independent reactivity in AIH‐1 samples. CONCLUSIONS: In rheumatoid arthritis and most non‐rheumatoid arthritis rheumatologic disease sera, anti‐CCP positivity is citrulline‐dependent. However in some patients, particularly patients with AIH‐1, citrulline‐independent reactivity in the anti‐CCP2 test can occur. A positive CCP test in a non‐rheumatic disease (eg liver disease) should therefore be interpreted with care, and preferably followed by a control ELISA with a non‐citrullinated antigen

Disease specificity of autoantibodies to cytosolic 5 '-nucleotidase 1A in sporadic inclusion body myositis versus known autoimmune diseases

Neurological Motor Disorder