518 research outputs found
Seven-fluorochrome mouse M-FISH for high-resolution analysis of interchromosomal rearrangements
The mouse has evolved to be the primary mammalian genetic model organism. Important applications include the modeling of human cancer and cloning experiments. In both settings, a detailed analysis of the mouse genome is essential. Multicolor karyotyping technologies have emerged to be invaluable tools for the identification of mouse chromosomes and for the deciphering of complex rearrangements. With the increasing use of these multicolor technologies resolution limits are critical. However, the traditionally used probe sets, which employ 5 different fluorochromes, have significant limitations. Here, we introduce an improved labeling strategy. Using 7 fluorochromes we increased the sensitivity for the detection of small interchromosomal rearrangements (700 kb or less) to virtually 100%. Our approach should be important to unravel small interchromosomal rearrangements in mouse models for DNA repair defects and chromosomal instability. Copyright (C) 2003 S. Karger AG, Basel
Random matrix theory for CPA: Generalization of Wegner's --orbital model
We introduce a generalization of Wegner's -orbital model for the
description of randomly disordered systems by replacing his ensemble of
Gaussian random matrices by an ensemble of randomly rotated matrices. We
calculate the one- and two-particle Green's functions and the conductivity
exactly in the limit . Our solution solves the CPA-equation of the
-Anderson model for arbitrarily distributed disorder. We show how the
Lloyd model is included in our model.Comment: 3 pages, Rev-Te
Rigorous mean field model for CPA: Anderson model with free random variables
A model of a randomly disordered system with site-diagonal random energy
fluctuations is introduced. It is an extension of Wegner's -orbital model to
arbitrary eigenvalue distribution in the electronic level space. The new
feature is that the random energy values are not assumed to be independent at
different sites but free. Freeness of random variables is an analogue of the
concept of independence for non-commuting random operators. A possible
realization is the ensemble of at different lattice-sites randomly rotated
matrices. The one- and two-particle Green functions of the proposed hamiltonian
are calculated exactly. The eigenstates are extended and the conductivity is
nonvanishing everywhere inside the band. The long-range behaviour and the
zero-frequency limit of the two-particle Green function are universal with
respect to the eigenvalue distribution in the electronic level space. The
solutions solve the CPA-equation for the one- and two-particle Green function
of the corresponding Anderson model. Thus our (multi-site) model is a rigorous
mean field model for the (single-site) CPA. We show how the Llyod model is
included in our model and treat various kinds of noises.Comment: 24 pages, 2 diagrams, Rev-Tex. Diagrams are available from the
authors upon reques
The Free Quon Gas Suffers Gibbs' Paradox
We consider the Statistical Mechanics of systems of particles satisfying the
-commutation relations recently proposed by Greenberg and others. We show
that although the commutation relations approach Bose (resp.\ Fermi) relations
for (resp.\ ), the partition functions of free gases are
independent of in the range . The partition functions exhibit
Gibbs' Paradox in the same way as a classical gas without a correction factor
for the statistical weight of the -particle phase space, i.e.\ the
Statistical Mechanics does not describe a material for which entropy, free
energy, and particle number are extensive thermodynamical quantities.Comment: number-of-pages, LaTeX with REVTE
Semigroups of distributions with linear Jacobi parameters
We show that a convolution semigroup of measures has Jacobi parameters
polynomial in the convolution parameter if and only if the measures come
from the Meixner class. Moreover, we prove the parallel result, in a more
explicit way, for the free convolution and the free Meixner class. We then
construct the class of measures satisfying the same property for the two-state
free convolution. This class of two-state free convolution semigroups has not
been considered explicitly before. We show that it also has Meixner-type
properties. Specifically, it contains the analogs of the normal, Poisson, and
binomial distributions, has a Laha-Lukacs-type characterization, and is related
to the case of quadratic harnesses.Comment: v3: the article is merged back together with arXiv:1003.4025. A
significant revision following suggestions by the referee. 2 pdf figure
Lowering and raising operators for the free Meixner class of orthogonal polynomials
We compare some properties of the lowering and raising operators for the
classical and free classes of Meixner polynomials on the real line
Towards many colors in FISH on 3D-preserved interphase nuclei
The article reviews the existing methods of multicolor FISH on nuclear targets, first of all, interphase chromosomes. FISH proper and image acquisition are considered as two related components of a single process. We discuss (1) M-FISH (combinatorial labeling + deconvolution + widefield microscopy); (2) multicolor labeling + SIM (structured illumination microscopy); (3) the standard approach to multicolor FISH + CLSM (confocal laser scanning microscopy; one fluorochrome - one color channel); (4) combinatorial labeling + CLSM; (5) non-combinatorial labeling + CLSM + linear unmixing. Two related issues, deconvolution of images acquired with CLSM and correction of data for chromatic Z-shift, are also discussed. All methods are illustrated with practical examples. Finally, several rules of thumb helping to choose an optimal labeling + microscopy combination for the planned experiment are suggested. Copyright (c) 2006 S. Karger AG, Basel
Applications of Automata and Graphs: Labeling-Operators in Hilbert Space I
We show that certain representations of graphs by operators on Hilbert space
have uses in signal processing and in symbolic dynamics. Our main result is
that graphs built on automata have fractal characteristics. We make this
precise with the use of Representation Theory and of Spectral Theory of a
certain family of Hecke operators. Let G be a directed graph. We begin by
building the graph groupoid G induced by G, and representations of G. Our main
application is to the groupoids defined from automata. By assigning weights to
the edges of a fixed graph G, we give conditions for G to acquire fractal-like
properties, and hence we can have fractaloids or G-fractals. Our standing
assumption on G is that it is locally finite and connected, and our labeling of
G is determined by the "out-degrees of vertices". From our labeling, we arrive
at a family of Hecke-type operators whose spectrum is computed. As
applications, we are able to build representations by operators on Hilbert
spaces (including the Hecke operators); and we further show that automata built
on a finite alphabet generate fractaloids. Our Hecke-type operators, or
labeling operators, come from an amalgamated free probability construction, and
we compute the corresponding amalgamated free moments. We show that the free
moments are completely determined by certain scalar-valued functions.Comment: 69 page
The Index of (White) Noises and their Product Systems
(See detailed abstract in the article.) We single out the correct class of
spatial product systems (and the spatial endomorphism semigroups with which the
product systems are associated) that allows the most far reaching analogy in
their classifiaction when compared with Arveson systems. The main differences
are that mere existence of a unit is not it sufficient: The unit must be
CENTRAL. And the tensor product under which the index is additive is not
available for product systems of Hilbert modules. It must be replaced by a new
product that even for Arveson systems need not coincide with the tensor
product
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