Computer Simulation of Excitation-Contraction Coupling in Cardiac Muscle. A Study of the Regulatory Role of Calcium Binding to Troponin C

Abstract. The influence of a change of troponin concentration as well of a change of binding and dissociation of Ca 2+ ions to the íegulatoiy piotem tioponin C on the time couise of isometiic tension has been studied using a mathematical model developed to investigate excitation-conti action coupling m caidiac muscle cells The numencal simulations show that peak amplitude, íate of foice development, time to peak tension and relaxation time depend significantly on the above paiameteis even m the case when the equilibrium dissociation constant lemams unchanged The obtained íesults might be useful foi the planing of new expenments in the view of the fact that no similai data have been íepoited foi caidiac muscle cells as ye

Observation of enhanced transmission for s-polarized light through a subwavelength slit

Enhanced optical transmission (EOT) through subwavelength apertures is usually obtained for p-polarized light. The present study experimentally investigates EOT for s-polarized light. A subwavelength slit surrounded on each side by periodic grooves has been fabricated in a gold film and covered by a thin dielectric layer. The excitation of s-polarized dielectric waveguide modes inside the dielectric film strongly increases the s-polarized transmission. Transmission measurements are compared with a coupled mode model and show good qualitative agreement. Adding a waveguide can improve light transmission through subwavelength apertures, as both s and p-polarization can be efficiently transmitted.Comment: 11 pages, 3 figures, submitted to Applied Physics Letter

Fundamentals of Diabetic Cataractogenesis and Promising Ways of its Pharmacological Correction

Cataracts in diabetes mellitus lead to decreased visual function and blindness. Cataract surgery for diabetes mellitus has limitations and complications. The search for effective means of conservative cataract therapy continues. The review presents the analysis of data from scientific sources, mainly for 2015–2020 using Internet resources (PubMed, Web of Science, Medline, eLibrary.Ru, Cyberleninka). In the work, diabetic cataractogenesis is presented as a sum of interrelated pathobiochemical processes. The main ones are the polyol pathway of glucose conversion, non-enzymatic glycation and oxidative modification of lens proteins, which are enhanced in diabetes mellitus. The lens has a high protein content. The formation of high molecular weight protein aggregates is of particular importance for the appearance of light scattering zones and a decrease in lens transparency. This review presents data on anti-cataract compounds that affect post-translational crystallin modification, prevent osmotic and oxidative stress in the lens, and exhibit antiglycation properties. This information shows that the search for means of pharmacological correction of cataractogenesis should be carried out among compounds with antioxidant and antiglycation activity

Changes in insulin like growth factors, myostatin and vascular endothelial growth factor in rat musculus latissimus dorsi by poly 3-hydroxybutyrate implants

The present study aimed at researching the synergistic effect between an ectopic bone substitute and surrounding muscle tissue. To describe this effect, changes of insulin like growth factors (IGF1, IGF2), myostatin (GDF8) and vascular endothelial growth factor (VEGF) mRNA content of 12 Wistar-King rats musculus latissimus dorsi with implanted poly-3-hydroxybutyrate (PHB) scaffold were examined after 6 and 12 weeks. At each time interval six rats were killed and implants and surrounding tissues prepared for genetic evaluation. Eight rats without any implants served as controls. RNAwas extracted from homogenized muscle tissue and reverse transcribed. Changes in mRNA content were measured by Real-Time PCR using specific primers for IGF1, IGF2, GDF8 and VEGF. Comparing the level of VEGF mRNA in muscle after 6 and 12 weeks to the controls, we could assess a significant increase of VEGF gene expression (