Constructing Optical Biosensor Based on Multienzyme System Tyrosinase/Horseradish Peroxidase

By using sol-gel method, hybrid membranes were synthesized based on SiO2 and cellulose derivatives. Membranes showed good physical and mechanical properties as well as good biocompatibility. Onto hybrid membranes tyrosinase and horse radish peroxidase were immobilized and optical biosensor was constructed. The working principle of constructed biosensor was detection of oxygen consumption. The properties of constructed biosensors like linear range, life time and sensitivity were defined by modeling substrate - L-3,4-dihydroxyphenylalanine. The factors affecting the constructed optical biosensors were investigated. The results showed that a multienzyme system is capable of improving the constructed biosensor properties

Covalent Immobilization of Peroxidase onto Hybrid Membranes for the Construction of Optical Biosensor

The aim of this study is to covalently immobilize horse radish peroxidase (HRP) onto new hybrid membranes synthesized by the sol-gel method based on silica precursors, dendrimers and cellulose derivatives. This new system will be used for designing biosensor. For investigation of the properties of membranes, HRP was used as a modeling enzyme. Kinetic parameters, pH and temperature optimum were determined, and the structure of the membranes surface was examined. Results showed higher relative and residual activity of HRP immobilized onto membranes with cellulose acetate butyrate with high molecular weight CAB/H. This novel biosensor could offer a simple, cheap and rapid tool with enhanced sensing performance as well as having potentials to find application in medicine, pharmacy, food and process control and environmental monitoring

Co-Immobilization of Peroxidase and Tyrosinase onto Hybrid Membranes Obtained by the Sol-Gel Method for the Construction of an Optical Biosensor

International audienc

Investigation on the proliferation of Gram negative bacterial cells onto sol-gel carriers

International audienceThis work is related to the synthesis and characterization of new hybrid sol-gel materials. Several carriers based on poly (N-acryloylglycine) (NAGly) composed of poly (ethylene glycol) dimethacrylate (PEGDM) were prepared. The effectiveness of biofilms formation of bacterial strains Escherichia coli and Pseudomonas fluorescents on the matrices was investigated by biochemical methods. Two types of hybrid gels were synthesized based on PEGDM, 2-hydroxyethyl acrylate (HEA) and N-acryloxysuccinimide ester (NAS) with incorporation of inorganic precursors. The rheological properties of the gels and formed biofilms were investigated by quartz crystal microbalance (qCM). The experimental results demonstrated that the obtained matrices are appropriate for biofilms formatio