Evaluation of iron and zinc levels in recurrent tonsillitis and tonsillar hypertrophy

WOS: 000372213200012PubMed ID: 26954864Objectives: The aim of this study is to look into the roles of iron and zinc metals in etiopathogenesis of recurrent tonsillitis and tonsillar hypertrophy by evaluating the levels of iron and zinc elements in the palatine tonsillar tissue. Methods: In total, 40 patients who underwent a tonsillectomy to treat recurrent tonsillitis and tonsillar hypertrophy were included in the study. Patients were classified into two groups, recurrent tonsillitis and tonsillar hypertrophy, determined by the results of clinical and histopathological examination. The levels of iron and zinc elements were determined for each tonsillar tissue sample. Results: There was a significant difference in the iron and zinc concentrations (p < 0.001) between the tonsillar hypertrophy and recurrent tonsillitis groups. The levels of iron and zinc were significantly lower in the recurrent tonsillitis group. Conclusions: This study suggests that low tissue concentrations of iron and zinc may lead to recurrent tonsillitis. (C) 2016 Elsevier Inc. All rights reserved

Curcumin protects against acoustic trauma in the rat cochlea

WOS: 000406729400019PubMed ID: 28688549Objectives: In this study we evaluated the therapeutic utility of curcumin in a rodent model of acoustic trauma using histopathology, immunohistochemical, and distortion product otoacoustic emission (DPOAEs) measurements. Methods: 28 Wistar albino rats were included in the study and randomly assigned to 4 treatment groups. The first group (group 1) served as the control and was exposed to acoustic trauma alone. Group 2 was the curcumin group. Group 3 was the curcumin plus acoustic trauma group. Group 4 was the saline plus acoustic trauma group. Otoacoustic emission measurements were collected at the end of the experiment and all animals were sacrificed. Cochlea were collected and prepared for TUNEL (TdT-mediated deoxyuridinetriphosphate nick end-labelling) staining assay. Results: Group 3 maintained baseline DPOAEs values at 3000 Hz, 4000 Hz and 8000 Hz on the 3rd and 5th day of the experiment. DPOAEs results were correlated with the immunohistochemical and histopathological findings in all groups. In comparison to the histopathologic control group, Group 1 exhibited a statistically significant increase in apoptotic indices in the organ of Corti, inner hair cell, and outer hair cell areas (p < 0.05). Relative to the control group, rats in Group 3 showed little increase in inner hair cell and outer hair cell apoptotic indices. Conclusions: Our results support the conclusion that curcumin may protect the cochlear tissues from acoustic trauma in rats. Curcumin injection prior to or after an acoustic trauma reduces cochlear hair cell damage and may protect against hearing loss. (C) 2017 Elsevier B.V. All rights reserved

Intraperitoneal curcumin and vitamin E combination for the treatment of cisplatin-induced ototoxicity in rats

WOS: 000383933500034PubMed ID: 27619052Introduction: Cisplatin ototoxicity is characterized by irreversible, progressive, bilateral sensorineural hearing loss at high frequencies, accompanied by tinnitus. The aim of this study is to demonstrate the protective action of curcumin alone or in combination with vitamin E against cisplatin-induced ototoxicity in animal models. Material and methods: The study included 42 rats. Experimental animals were randomized into 6 groups. In the first group, intra-peritoneal cisplatin was administered alone. In the second group, intra-peritoneal cisplatin and curcumin were administered together. In the third group, intra-peritoneal cisplatin and vitamin E were administered together. In the fourth group, intra-peritoneal cisplatin was administered together with curcumin in combination with vitamin E. In the fifth group, intra-peritoneal curcumin was administered alone. The sixth group was sacrificed directly without administration of any drugs. A distortion product otoacoustic emission (DPOAE) test was applied to both ears of all experimental animals. Curcumin was administered 1 h before cisplatin treatment continued for three successive days. Vitamin E was administered only as a single dose 30 min prior to cisplatin. All animals were sacrificed following DPOAE testing on the 5th day of cisplatin administration. Histopathological findings included a TUNEL (TdT-mediated deoxyuridine triphosphate nick end-labeling) assay, and the percentage of apoptotic cells was calculated. DPOAE values and the percentage of apoptotic cells were compared before and after treatment and between experimental groups. Results: In Group 1, DPOAE values were significantly decreased at all frequencies (3000 Hz, 4000 Hz and 6000 Hz; P 0.05). Apoptotic index values were lower in all treatment groups compared to the cisplatin group, however the difference was only statistically significant in group 3 (p = 0.009). Conclusion: In rats, cisplatin ototoxicity can be prevented with curcumin or curcumin-vitamin E combination. (C) 2016 Elsevier Ireland Ltd. All rights reserved

Comparison of Endoscopic and External Dacryocystorhinostomy Results and Analysis of Patients' Satisfaction

WOS: 000386244200003PubMed ID: 29392027Objective: Comparison of endoscopic and external dacryocystorhinostomy (DCR) results and evaluation of patients' satisfaction. Methods: Forty six (35 females and 11 males) patients who underwent endoscopic DCR and 43 (37 females and six males) who underwent external DCR were included. Surgical success was objectively and subjectively assessed. The nasolacrimal duct was irrigated by a saline solution, and the saline solution was objectively visualized by endoscopy from the nose. Subjective assessment was performed asking the patients' epiphora. In addition to evaluating the success of the operation, satisfaction and result surveys were administered to the two groups. Results: There was no statistically significant difference between the two groups in terms of age and sex (respectively p=0.486, p=0.23). However, the number of females was higher than the number of males in the two groups, and the difference was statistically significant (endoscopic-DCR p=0.01, external-DCR p=0.001). There was no statistically significant difference between the two groups in terms of postoperative bleeding and punctum damage. The success rate was 84.7% in the endoscopic DCR group and 90.6% in the external DCR group. There was no statistically significant difference in the success rate between the two groups (p=0.397). The survey results revealed that there was no statistically significant difference between the two groups in terms of patient satisfaction (p=0.397). Conclusion: The results of many studies in the literature show operation success rates between the two groups that are similar to ours. Both techniques have advantages and disadvantages. Independent of the preferred procedure, our results show that functional success mainly determines patient satisfaction

MBL, P2X7, and SLC11A1 gene polymorphisms in patients with oropharyngeal tularemia

WOS: 000386069300016PubMed ID: 27223255Conclusion: A significant association was found of oropharyngeal tularemia with SLC11A1 allele polymorphism (INT4G/C) and MBL2 C+4T (P/Q). These results indicate C allele and Q allele might be a risk factor for the development of oropharyngeal tularemia.Aim: This study aimed to investigate the relationship of SLC11A1, MBL, and P2X(7) gene polymorphism with oropharyngeal tularemia.Methods: The study included totally 120 patients who were diagnosed with oropharyngeal tularemia. Frequencies of polymorphisms in the following genes were analyzed both in the patient and control groups in the study: SLC11A1 (5'(GT)(n) Allele 2/3, Int4G/C, 3' UTR, D543N G/A), MBL (MBL2 C+4T (P/Q), and P2X(7) (-762 C/T and 1513 A/C).Results: Among all polymorphisms that were investigated in this study, SLC11A1 gene showed a significance in the distriburtion of polymorphism allelle frequency at the INT4 region. Frequency of C allele was 54 (28%) in patients with oropharyngeal tularemia, and 31 (13%) in the control group (p=0.006 and OR = 1.96 (1.21-3.20)). An association was detected between MBL2 C+4T (P/Q) gene polymorphism and oropharyngeal tularemia (p0.05).Gaziosmanpasa UniversityGaziosmanpasa University [2014/14]This study was supported by the Gaziosmanpasa University (project no: 2014/14)