169 research outputs found
The chromosomal association/dissociation of the chromatin insulator protein Cp190 of Drosophila melanogaster is mediated by the BTB/POZ domain and two acidic regions
<p>Abstract</p> <p>Background</p> <p>Chromatin insulators or boundary elements are a class of functional elements in the eukaryotic genome. They regulate gene transcription by interfering with promoter-enhancer communication. The Cp190 protein of <it>Drosophila </it><it>melanogaster </it>is essential to the function of at least three-types of chromatin insulator complexes organized by Su(Hw), CTCF and BEAF32.</p> <p>Results</p> <p>We mapped functional regions of Cp190 in vivo and identified three domains that are essential for the insulator function and for the viability of flies: the BTB/POZ domain, an aspartic acid-rich (D-rich) region and a C-terminal glutamic acid-rich (E-rich) region. Other domains including the centrosomal targeting domain and the zinc fingers are dispensable. The N-terminal CP190BTB-D fragment containing the BTB/POZ domain and the D-rich region is sufficient to mediate association with all three types of insulator complexes. The fragment however is not sufficient for insulator activity or viability. The Cp190 and CP190BTB-D are regulated differently in cells treated with heat-shock. The Cp190 dissociated from chromosomes during heat-shock, indicating that dissociation of Cp190 with chromosomes can be regulated. In contrast, the CP190BTB-D fragment didn't dissociate from chromosomes in the same heat-shocked condition, suggesting that the deleted C-terminal regions have a role in regulating the dissociation of Cp190 with chromosomes.</p> <p>Conclusions</p> <p>The N-terminal fragment of Cp190 containing the BTB/POZ domain and the D-rich region mediates association of Cp190 with all three types of insulator complexes and that the E-rich region of Cp190 is required for dissociation of Cp190 from chromosomes during heat-shock. The heat-shock-induced dissociation is strong evidence indicating that dissociation of the essential insulator protein Cp190 from chromosomes is regulated. Our results provide a mechanism through which activities of an insulator can be modulated by internal and external cues.</p
Pharmacy & Therapeutics Update: Drug Information for Health Care Professionals, May 2009
https://medica-musc.researchcommons.org/musc-ptupdate/1032/thumbnail.jp
Pharmacy & Therapeutics Update: Drug Information for Health Care Professionals
https://medica-musc.researchcommons.org/musc-ptupdate/1001/thumbnail.jp
Pharmacy & Therapeutics Update: Drug Information for Health Care Professionals, September 2009
https://medica-musc.researchcommons.org/musc-ptupdate/1035/thumbnail.jp
Pharmacy & Therapeutics Update: Drug Information for Health Care Professionals, November/December 2009
https://medica-musc.researchcommons.org/musc-ptupdate/1037/thumbnail.jp
Pharmacy & Therapeutics Update: Drug Information for Health Care Professionals, July/August 2009
https://medica-musc.researchcommons.org/musc-ptupdate/1034/thumbnail.jp
Pharmacy & Therapeutics Update: Drug Information for Health Care Professionals
https://medica-musc.researchcommons.org/musc-ptupdate/1000/thumbnail.jp
Pharmacy & Therapeutics Update: Drug Information for Health Care Professionals, July/August 2009
https://medica-musc.researchcommons.org/musc-ptupdate/1034/thumbnail.jp
Pharmacy & Therapeutics Update: Drug Information for Health Care Professionals, April 2009
https://medica-musc.researchcommons.org/musc-ptupdate/1031/thumbnail.jp
Pharmacy & Therapeutics Update: Drug Information for Health Care Professionals
https://medica-musc.researchcommons.org/musc-ptupdate/1001/thumbnail.jp
- …