Consumer attitudes and preference exploration towards fresh-cut salads using best–worst scaling and latent class analysis

This research explored the preferences and buying habits of a sample of 620 consumers of fresh-cut, ready-to-eat salads. A best–worst scaling approach was used to measure the level of preference stated by individuals regarding 12 attributes for quality (intrinsic, extrinsic and credence) of fresh-cut salads. The experiment was carried out through direct interviews at several large-scale retail outlets in the Turin metropolitan area (north-west of Italy). Out of the total number of questioned consumers, 35% said they did not consume fresh-cut salads. On the contrary, the rest of the involved sample expressed the highest degree of preference towards the freshness/appearance attribute, followed by the expiration date and the brand. On the contrary, attributes such as price, organic certification and food safety did not emerge as discriminating factors in consumer choices. Additionally, five clusters of consumers were identified, whose preferences are related both to purchasing styles and socio-demographic variables. In conclusion, this research has highlighted the positive attitude of consumers towards quality products backed by a brand, providing ideas for companies to improve within this sector and implement strategies to answer the needs of a new segment of consumers, by determining market opportunities that aim to strengthen local brands

Ecological and economic indicators for the evaluation of almond (Prunus dulcis l.) orchard renewal in sicily

Globally, almond production is experiencing a growing trend thanks to a strong interest in the health, gastronomic, and industrial properties that are linked to the fruits and their derivatives. After a constant and marked decline in the second half of the last century, the Mediterranean Basin is thoroughly reassessing this crop, which provides significant results with a modern orchard management. The opportunity determined by the transition from traditional to modern systems have increased the interest in evaluating the different environmental impacts of the two cultivation models that still coexist today. In this work, through the application of the Life Cycle Assessment (LCA) and an economic assessment approach with an in-depth analysis related to the cultivation cycle and the use of resources, the role played by each factor involved in production was determined. Overall, the Greenhouses Gases (GHG) emissions in modern farms are higher (Global Warming Potential (GWP) 0.224 kg CO2 eq.) than those in traditional farms (GWP 0.182 kg CO2 eq.). Regarding the economic assessment, it appears relevant that the modern almond model in the full production phase guarantees significantly higher margins (+84%).The perception of the importance attributed to evaluating economic and environmental aspects by different stakeholders shows relevant differences in the approach coming from growers, dealers, and governmental institutions allows the investigators to positively underline the current innovation in almond orchard systems considering the balance between fruit production and the conservation of environmental resources

A combined genome-wide approach identifies a new potential candidate marker associated with the coat color sidedness in cattle

Coat color is one of the most important phenotypic features in livestock breeds. Cinisara is a local cattle breed generally of uniform black color which occasionally presents a particular phenotype, with animals typically display a white band along their spine, from the head to the tail, and on the ventral line (color sidedness). Therefore, this breed provides an ideal model to study the genetic components underlying phenotypic variation in coat color. A total of 63 animals, ten with sidedness phenotype and 53 with uniform black color were genotyped with Illumina Bovine 50 K. The comparison among genome-wide association study and FST analysis revealed a single nucleotide polymorphism (SNP), ARS-BFGL-NGS-55928, significantly associated with the trait. Only one gene (PLK2)was annotated near the associated SNP in a window of ±200 kb. The protein encoded by this gene is a member of the polo-like kinases, the same family of several known coat-color candidate genes. Based on the reported results, we draw the possible conclusion that the identified marker is potentially associated with the coat color sidedness in Cinisara. The local breeds with their genetic variability represent an important resource and model to study the genetic basis affecting peculiar traits. Future studies would be particularly relevant to refine these results and to better understand the genetic basis for this phenotype

Expression of a SOX1 overlapping transcript in neural differentiation and cancer models

SOX1 is a member of the SOXB1 subgroup of transcription factors involved in early embryogenesis, CNS development and maintenance of neural stem cells. The structure and regulation of the human SOX1 locus has been less studied than that of SOX2, another member of the SOXB1 subgroup for which an overlapping transcript has been reported. Here we report that the SOX1 locus harbours a SOX1 overlapping transcript (SOX1-OT), and describe expression, splicing variants and detection of SOX1-OT in different stem and cancer cells. RT-PCR and RACE experiments were performed to detect and characterise the structure of SOX1-OT in neuroprogenitor cultures and across different cancer cell lines. SOX1-OT was found to present a complex structure including several unannotated exons, different transcript variants and at least two potential transcription start sites. SOX1-OT was found to be highly expressed in differentiated neural stem cells across different time points of differentiation, and its expression correlated with SOX1 gene expression. Concomitant expression of SOX1 and SOX1-OT was further observed in several cancer cell models. While the function of this transcript is unknown, the regulatory role reported for other lncRNAs strongly suggests a possible role for SOX1-OT in regulating SOX1 expression, as previously observed for SOX2. The elucidation of the genetic and regulatory context governing SOX1 expression will contribute to clarifying its role in stem cell differentiation and tumorigenesis

Transversals to Line Segments in Three-Dimensional Space

We completely describe the structure of the connected components of transversals to a collection of n line segments in R3. We show that n \u3e 3 arbitrary line segments in R3 admit 0, 1, . . . , n or infinitely many line transversals. In the latter case, the transversals form up to n connected components

Andricus cydoniae Giraud, 1859 Junior Synonym of Cynips coni-fica Hartig, 1843, as Experimentally Demonstrated (Hymenoptera: Cynipidae: Cynipini)

We demonstrated the life cycle closure of Cynips conifica Hartig, 1843 (presently Andricus conifi-cus), previously supposed on the basis of molecular data, and the identity of the sexual genera-tion, through laboratory experiments. As a consequence, Andricus cydoniae Giraud, 1859 became a junior synonym of A. conificus (Hartig, 1843). We provide illustrations and a diagnosis for adults and galls, observations on biology, and information on distribution. Moreover, as sexual galls of A. conificus cannot be distinguished from those of Andricus multiplicatus, a detailed com-parison between sexual galls and adults of these two species is reported

Dietary Patterns at the Individual Level through a Nutritional and Environmental Approach: The Case Study of a School Canteen

The public catering sector has important responsibilities in seeking a change toward more sustainable choices for many aspects related to the environmental impacts of their services. The environmental impact of production processes can be studied through life cycle assessment (LCA), which allows a greater awareness of choices and has rarely been applied to catering. In this work, we studied the impacts of two dishes (braised meat and cauliflower meatballs) in a school canteen, their impacts were studied using the daily energy requirement (expressed in kcal) as a functional unit. Global warming potential (GWP) and nonrenewable energy (NRE) were calculated starting from the supply of raw materials up to distribution. Electricity and the act of cooking the meatballs accounted for more than 60% of the measured impact in terms of GWP, whereas, less markedly, they dominated in terms of nonrenewable energy used. In the case of braised meat, the total impact was, however, attributable to the life cycle of the meat (between 60% and 76%) and the consumption of electricity (between 19% and 27%), whereas for all other factors, the contribution was never particularly high. Additionally, a discussion on the correct functional unit to be used proposed the environmental impact of different recipes as an additional criterion for nutritionists during the composition of the menu. An integrated system appears important for changing policies and behaviors and the application of LCA can be a tool capable of contributing to the construction of a holistic instrument of sustainability