Population dynamics of Meloidogyne incognita on cucumber grafted onto the Cucurbita hybrid RS841 or ungrafted and yield losses under protected cultivation

The influence of the squash hybrid RS841 rootstock (Cucurbita maxima x C. moschata) on population dynamics of Meloidogyne incognita and yield of cucumber cv. Dasher II was assessed during 2013 and 2014 in a plastic greenhouse. In addition, the relationship between ecophysiological parameters (plant water status, gas exchange, and leaf reflectance) and Pi and cucumber yield were also estimated in 2013. Nematode densities were determined at the beginning (Pi) and at the end (Pf) of each crop, and the relationship between these parameters was used to estimate the maximum multiplication rate (a), the maximum population density (M) and the equilibrium density (E) per grafted and ungrafted cucumber and cropping season. Moreover, the relationship between the multiplication rate (Pf/Pi) and Pi was compared between grafted and ungrafted cucumber per cropping season. Finally, the relative yield of grafted or ungrafted cucumber was plotted against Pi to determine the tolerance limit (T) and the minimum relative yield (m) by the Seinhorst damage function model. Values of a, M and E in grafted cucumber were higher than in ungrafted one irrespective of the cropping season. These results were supported by comparing the relationship between Pf/Pi and Pi between grafted and ungrafted cucumber. The relationship between Pi and yield fitted the Seinhorst damage function. The values of T and m did not differ between grafted and ungrafted each year. Predawn water potential, net photosynthetic rate, and leaf chlorophyll index decreased with increasing Pi. In addition, relative yield was related to variation in net photosynthetic rate and the leaf chlorophyll index. Under the conditions of this study, RS841 rootstock was neither resistant nor tolerant to M. incognita.Postprint (published version

Resistencia al nematodo Meloidogyne en patrones de tomate a temperaturas del suelo superiores a los 28ºC

El gen Mi en tomate confiere resistencia al nematodo Meloidogyne, aunque su eficacia disminuye a temperaturas superiores a los 28ºC. Para determinar la resistencia relativa de 12 patrones y cultivares de tomate frente a M. arenaria y M. javanica se realizaron ensayos en un invernadero sin control climático donde las plantas estaban sujetas a fluctuaciones diarias de temperatura. En el transcurso de los ensayos, la temperatura del suelo fue superior a 28ºC durante 31 días en el primer ensayo (Exp.1) y durante 20 días en el segundo (Exp. 2). El intervalo de tiempo en que la temperatura se mantuvo por encima de los 28oC osciló entre 0,5 a 6 horas/día en el Exp.1 y entre 0,5 a 7,5 horas/día en Exp. 2. Los patrones Morgan, King Kong y Unifort consistentemente mostraron alta resistencia frente a M. arenaria y M. javanica en ambos experimentos, incluso con picos de temperaturas superiores a 28ºC durante parte del experimento. Los patrones Multifort y Maxifort expresaron menor grado de resistencia que los anteriores bajo las mismas condiciones experimentales. En general, la tasa de multiplicación de M. javanica fue mayor (P<0,05) que la de M. arenaria tanto en los tomates resistentes como en los susceptibles.Postprint (published version

Quantitative approach for the early detection of selection for virulence of Meloidogyne incognita on resistant tomato in plastic greenhouses

Resistant tomato cultivars are an important tool to control Meloidogyne spp., which cause the highest yield losses attributed to plant-parasitic nematodes. However, the repeated cultivation of Mi resistant cultivars can select virulent populations. In the present study, the susceptible tomato cv. Durinta and the resistant cv. Monika were cultivated from March to July in a plastic greenhouse for 3 years to determine the maximum multiplication rate, maximum nematode density, equilibrium density, relative susceptibility and population growth rate of M. incognita; these were used as proxy indicators of virulence and yield losses. The values of population dynamics and growth rate on the resistant tomato increased year by year and were higher when it was repeatedly cultivated in the same plot compared to when it was alternated with the susceptible cultivar and the level of resistance decreased from very to moderately resistant. The relationship between the nematode density at transplanting (Pi) and the relative yield of tomato fitted to the Seinhorst damage model for susceptible, but not resistant, cultivars. The tolerance limit and the relative minimum yield were 2–4 J2 per 250 cm3 of soil and 0.44–0.48, respectively. The tomato yield did not differ between cultivars at low Pi, but it did at higher Pi values, at which the resistant yielded 50% more than the susceptible. This study demonstrates the utility of population dynamics parameters for the early detection of selection for virulence in Meloidogyne spp., and that three consecutive years were not sufficient to select for a completely virulent population.Postprint (author's final draft

Efectiveness of BIONEM WP against Meloidogyne javanica on cucumber and tomato crops in a plastic house

Postprint (published version

Response of potential cucurbit rootstocks against Meloidogyne spp

the Cucurbita hybrids, usually used as cucurbit rootstocks, are susceptible to Meloidogyne. Its cultivation increases the nematode population densities at similar levels than ungrafted cucumber or melon crops, and higher 'Ievels than watermelon.Postprint (published version

Development of a nematicidal extract from Paecilomyces lilacinus

Postprint (published version

Grafting melons onto potential cucumis spp. rootstocks

Cucumís melo is an economically importar]t crop. Its culture is hampered by differen t types of soil stresses. Grafting melons onto different resistant cucurbits belonging to the genera, Cucurbíta, Lagenaría, Luffa, etc. have been successfully used to avoid these problems. However, me Ion quality has been nega.tively modified as a consequence of grafting. In general, variation in fruit shape, seed cavity and sugar content have been observed. The use of rootstocks more genetically c\oser to the melon scions could be useful to obtain fru i ts with better quality from melon grafted plantsPostprint (published version

Effectiveness and profitability of the mi-resistant tomatoes to control root-knot nematodes

Peer Reviewe

Screening of potencial biological control agents with nematicide action

Postprint (published version

La actual situación financiera y fiscal de la Iglesia Católica en España: propuestas de reforma a la luz de la experiencia comparada.

La cooperación económica entre el Estado y las confesiones religiosas, y la financiación pública que el primero ofrece a estas, es un asunto de gran importancia en nuestra sociedad. Esto es lo que analizaremos en este trabajo. Nos centraremos en el caso de la Iglesia Católica, fe mayoritaria en España, estudiando las relaciones que mantiene actualmente con el Estado y cómo han evolucionado las mismas. También analizaremos la situación de las restantes confesiones minoritarias y recurriremos al análisis de la experiencia internacional comparada para formular propuestas de mejora basadas en los principios económicos de suficiencia, autonomía y equidad, a la necesaria separación Iglesia-Estado y al derecho de libertad religiosa