Isotopic Characteristics and Water Interaction of Ifni Lake and Spring of Tifnoute Valley (High Atlas Mountains, Morocco, North of Africa)

Geochemical and isotopic investigations were carried out to indicate the interaction process between Ifni Lake water and high valley of Tifnoute springs. A total of 18 water samples were collected and analyzed. The Ifni Lake constitutes a veritable water resource in the study area, and this water can be the origin of alimentation the springs located in the high valley the Tifnoute. This study aims to improve the interaction between Ifni Lake water and the springs of high valley of Tifnoute. It is mainly focused to identify the origin of groundwater recharge and mineralization. The environmental isotope (deuterium (δ2H) and oxygen-18 (δ18O)) measurements allowed understanding the hydro geochemical process, the origin of the mineralization of the water. Also the results identify the relationship between the waters of Ifni Lake and the spring's water of high valley of Tifnoute. The isotopic compositions reveal that the origin of Ifni Lake water is meteoric, and the water infiltrates directly in the groundwater without any evaporation

Isolation and localization of cells expressing Sca-1 in the Adult Mouse Ovary: An evidence for presence of Mesenchymal Stem cells

Objective: Recently growing evident declared that ‘neo-oogenesis’ continues in mature female life span and simultaneously another studies confirmed the presence of spermatogonial stem cells (SSCs). Even though there is agreement between scientist about SSCs population in male gender but on the other side ovarian stem cells have received raising challenges regarding the existence in the surface epithelium of ovary. Mesenchymal stem cells (MSCs) are the most applicable source of stem cells and the common marker of MSCs is Sca-1 so the purpose of this study was to clarify the incidence of stem cells in the surface epithelium of ovary Methods: forty C57BL6 mice were sacrificed and the ovary carefully excised from its surrounding fat tissue, after mechanical and enzymatic digestion cells were stained with Sca1 to estimate the incidence of positive stem cells (SCs) population fluorescence activated cell sorting (FACS). Part of digested cells used for RT-PCR, also histological section prepared for immunohistochemical (IHC) staining of Sca-1 in ovarian surface epithelium (OSE) FACS. Results: The gene expression of Sca-1 was confirmed in the ovarian tissue. As well, localization of Sca-1 positive cells was detected in the germinal layer of ovary and epithelial granular layer of primordial follicles. Moreover, we successfully could isolated the Sca-1positive cells through Conclusion: The present work findings confirmed an inclusive stem cell population in the ovary which can be a strong evident for regeneration of ovarian tissue in either purpose of ovulation scar and neo-oogenesis

Attitude of public health students towards their field of study and future professions in Lorestan University of Medical Sciences

Introduction: Field of study and future profession has always been an important subject of education. The purpose of this study was identifying the attitude of public health students at Lorestan University of Medical Sciences toward field of study and future profession. Methods: This study is a cross-sectional. 78 students of public health from third to eighth semester in the academic year 2018 the population were selected by census method. The questionnaire used in this study is derived from the American University of Minnesota Attitude Questionnaire. It was developed in two sections (demographic characteristics & 17 questions about students̕ attitudes & perspectives toward field of study and future career). To analyze the data T-test, One-way ANOVA, Pearson correlation were used. Results: The mean score±standard deviation of students’ attitude toward the field of study and future career was 27.14±5.1 (theoretical mean score=27) and 22.79±5.25 (theoretical mean score =24) respectively. There was a significant correlation between their attitude towards field of study and their attitude towards future career (r = 0.472, P<0.0001). Important factors that gave rise to a negative attitude towards the future professions were inconsistencies between the curriculum of Public Health program and needs of society, anxiety about future profession, inadequate salary and employment benefits. Main negative factors related to students' attitudes about field of study were the negative attitude of society and other disciplines towards this field of study Conclusion: Regarding the results, attitudes of student about their future career and field of study were negative (close to average). Reforming and strengthening educational program, improvement of students’ internship, student admission according to society needs and establishing desirable conditions for graduates’ employment in this field lead to increase students' positive attitudes towards their field of study and consequently improve their attitude towards their future professions