Mise en place d'un système d'assurance qualité au sein d'une officine

PARIS-BIUP (751062107) / SudocSudocFranceF

First case report of intrathecal panitumumab for treatment of meningeal carcinomatousis in an EGFR mutant lung adenocarcinoma patient

International audienceIntrathecal (IT) chemotherapy has been the mainstay treatment for patients with meningeal carcinomatosis (MC) but the prognosis of MC remains disastrous and most patients expire before 6 months [1]. Recently IT administration of trastuzumab, an IgG1 anti-HER2 monoclonal antibody (mAbs), has been tested with signs of efficacy in HER2 positive breast cancer patients [2–5]. We report here the first case of IT panitumumab, a human recombinant IgG2 kappa mAbs that binds specifically to EGFR, for a patient with MC derived from an EGFR exon 19 mutant lung adenocarcinoma

IRAP, a retrotransposon-based marker system for the detection of somaclonal variation in barley

The retrotransposon-based marker system, inter-retrotransposon amplified polymorphism (IRAP), and inter-simple sequence repeats (ISSRs) were used to detect somaclonal variation induced by tissue culture. IRAPs use a single primer designed to amplify out from the 5′ LTR sequence of the BARE-1 retrotransposon combined with a degenerate 3′ anchor, similar to that of ISSR primers. We analysed DNA polymorphisms in 147 primary regenerants and parental controls from three cultivars of barley (Hordeum vulgare). The ISSR marker system generated an average of 218 bands per primer, with 29 polymorphisms of which 12 were novel non-parental bands. In comparison, the IRAP system generated an average of 121 bands per primer, with 15 polymorphisms of which nine were novel non-parental bands. Polymorphism detected for IRAP and ISSR markers was more than twofold higher in Golden Promise than Mackay and Tallon cultivars. However, there was no significant difference in the frequency of novel non-parental bands. Cluster analysis revealed that the level of polymorphism and genetic variability detected was comparable between IRAP and ISSR markers. This suggests that retrotransposon-based marker systems, such as IRAP, based on retrotransposons such as BARE-1, are valuable tools for the detailed characterisation of mutation profiles that arise during tissue culture. Their use should improve our understanding of processes influencing mutation and somaclonal variation and allow for the design of methods that yield fewer genome changes in applications where maintaining clonal integrity is important