SFGP 2007 - Investigation of a Novel Principle of Chemical Grafting for Modification of Cellulose Fibers

Natural cellulose fibres have been employed for packaging applications for a long time. Their use, however, has been hampered by their high hydrophilicity and their moisture sensitivity. It has, thus, been proposed to circumvent this problem through the hydrophobic modification of their surface thanks to the use of molecular grafting approaches. In this work, we describe the use of a novel solvent-free chemical pathway for molecular grafting that we have coined chromatogenic chemistry. It involves a reaction between a solid substrate and a reagent which is in a vapour-liquid equilibrium and diffuses within the solid substrate through a mechanism of adsorption/desorption akin to gas chromatography. Chromatogenic chemistry phenomenon has been studied and modelled through the extensive use of a new specific test, the Droplet Surface Migration Test. It involves the deposition upon a porous substrate of a small amount of reagent and in studying its subsequent migration and grafting. Whatman paper and various long chain acid chlorides were used for this modelling. The acid chloride carboxylic ends react with the external hydroxyl groups of cellulose fibres to give rise to the formation of long chain hydrophobic ester bonds. Upon immersion of the paper sheet in distilled water, a hydrophobic spot, extending well over the initial depot zone, could then be clearly visualized, allowing to follow conveniently the reagent migration and reaction. Grafting densities were performed by using the HPLC technique. The results obtained through the use of this test allowed a better understanding of chromatogenic chemistry phenomenon and an identification of the main parameters which affect the process: the nature of the reagent, the temperature, the reaction time, the nature of the substrate, etc. We have more particularly shown that the diffusion and grafting yields were maximal for a specific temperature which increases with the boiling point and therefore with the chain length of the reagents. We have proposed that this temperature should correspond to a compromise between the diffusion and reactivity properties of the reagent, its evaporation and its degradation by hydrolysis

MNF, an ankyrin repeat protein of myxoma virus, is part of a native cellular SCF complex during viral infection

Myxoma virus (MYXV), a member of the Poxviridae family, is the agent responsible for myxomatosis, a fatal disease in the European rabbit (Oryctolagus cuniculus). Like all poxviruses, MYXV is known for encoding multiple proteins that regulate cellular signaling pathways. Among them, four proteins share the same ANK/PRANC structure: M148R, M149R, MNF (Myxoma Nuclear factor) and M-T5, all of them described as virulence factors. This family of poxvirus proteins, recently identified, has drawn considerable attention for its potential role in modulating the host ubiquitin-proteasome system during viral infection. To date, many members of this novel protein family have been shown to interact with SCF components, in vitro. Here, we focus on MNF gene, which has been shown to express a nuclear protein presenting nine ANK repeats, one of which has been identified as a nuclear localization signal. In transfection, MNF has been shown to colocalise with the transcription factor NF-!B in the nucleus of TNFa-stimulated cells. Functionally, MNF is a critical virulence factor since its deletion generates an almost apathogenic virus. In this study, to pursue the investigation of proteins interacting with MNF and of its mechanism of action, we engineered a recombinant MYXV expressing a GFP-linked MNF under the control of MNF native promoter. Infection of rabbits with MYXV-GFPMNF recombinant virus provided the evidence that the GFP fusion does not disturb the main function of MNF. Hence, cells were infected with MYXV-GFPMNF and immunoprecipitation of the GFPMNF fusion protein was performed to identify MNF’s partners. For the first time, endogenous components of SCF (Cullin-1 and Skp1) were co-precipitated with an ANK myxoma virus protein, expressed in an infectious context, and without over-expression of any protein

M148R and M149R are two virulence factors for myxoma virus pathogenesis in the European rabbit

Myxoma virus (MYXV), a member of the Poxviridae family, is the agent responsible for myxomatosis, a fatal disease in the European rabbit (Oryctolagus cuniculus). MYXV has a linear double-stranded DNA genome that encodes several factors important for evasion from the host immune system. Among them, four ankyrin (ANK) repeat proteins were identified: M148R, M149R, M150R and M-T5. To date, only M150R and M-T5 were studied and characterized as critical virulence factors. This article presents the first characterization of M148R and M149R. GFP fusions allowed us to localize them in a viral context. Whereas M149R is only cytoplasmic, interestingly, M148R is in part located in the nucleolus, a unique feature for an ANK repeat poxviral protein. In order to evaluate their implication in viral pathogenicity, targeted M148R, M149R, or both deletions were constructed in the wild type T1 strain of myxoma virus. In vitro infection of rabbit and primate cultured cells as well as primary rabbit cells allowed us to conclude that M148R and M149R are not likely to be implicated in cell tropism or host range functions. However, in vivo experiments revealed that they are virulence factors since after infection of European rabbits with mutant viruses, a delay in the onset of clinical signs, an increase of survival time and a dramatic decrease in mortality rate were observed. Moreover, histological analysis suggests that M148R plays a role in the subversion of host inflammatory response by MYXV

Lattice strain measurements using synchrotron diffraction to calibrate a micromechanical modeling in a ferrite–cementite steel

In situ tensile tests were performed at room temperature on a ferrite–cementite steel specifically designed for this study. The evolution of the average stress in ferrite during loading was analyzed by X-ray diffraction.Lattice strain measurements were performed with synchrotron ring diffraction in both ferrite and cementite.These in situ tests were complemented by macroscopic tensile and reversible tensile-compression tests to study the Bauschinger effect. In order to reproduce stresses in ferrite and cementite particles,a recently developed micromechanical Internal Length Mean Field (ILMF) model based on a generalized self-consistent scheme is applied. In this designed ferrite–cementite steel,the third ‘‘phase’’of the model represents finite intermediate‘‘layers’’in ferrite due to large geometrically necessary dislocation (GND) densities around cementite particles. The assumed constant thickness of the layers is calibrated thanks to the obtained experimental data.The ILMF model is validated by realistic estimates of the Bauschinger stress and the large difference between mean stresses in ferrite and in cementite phases.This difference cannot be reproduced by classic two-phase homogenization schemes without intermediate GND layers

Occupational Activities and Cognitive Reserve: a Frontier Approach Applied to the Survey on Health, Ageing, and Retirement in Europe

The aim of this paper was to use a parametric stochastic frontier approach (coming from the economic literature) to explore the impact of the concept of activity (taken in a broad sense: i.e., including both professional and non-professional activities) on the constitution and the care of cognitive reserve among the European population aged 50 and up. For this purpose, we use individual data collected during the first wave of SHARE (Survey on Health, Ageing and Retirement in Europe) performed in 2004. The advantages of this survey were (1) it included a large population (n = 18,623) geographically distributed throughout Europe; and (2) it simultaneously analyzed several dimensions (physical and mental health, mobility, occupational activities, socioeconomic status, etc.). Our results confirm the positive impact of occupational activities on the cognitive functioning of elderly people. These results are discussed in terms of the prevention of cognitive aging and Alzheimer’s disease, and more particularly of retirement policy issues.

Abundance Patterns in S-type AGB stars : Setting Constraints on Nucleosynthesis and Stellar Evolution Models

During the evolution on the AGB, S-type stars are the first objects to experience s-process nucleosynthesis and third dredge-ups, and therefore to exhibit sprocess signatures in their atmospheres. Their significant mass loss rates (10^-7 to 10^-6 M*/year) make them major contributors to the AGB nucleosynthesis yields at solar metallicity. Precise abundance determinations in S stars are of the utmost importance for constraining e.g. the third dredge-up luminosity and efficiency (which has been only crudely parameterized in all current nucleosynthetic models so far). Here, dedicated S-star model atmospheres are used to determine precise abundances of key s-process elements, and to set constraints on nucleosynthesis and stellar evolution models. A special interest is paid to technetium, an element with no stable isotopes (99Tc, the only isotope produced by the s-process in AGB stars, has a half-life of 2.1 x 10^5 years). Its detection is considered as the best signature that the star effectively populates the thermally-pulsing AGB phase of evolution. The derived Tc/Zr abundances are compared, as a function of the derived [Zr/Fe] overabundances, with AGB stellar model predictions. The [Zr/Fe] overabundances are in good agreement with the model predictions, while the Tc/Zr abundances are slightly overpredicted. This discrepancy can help to set better constraints on nucleosynthesis and stellar evolution models of AGB stars.Comment: 5 pages, 3 figures, To be published in the proceedings of the conference "Why Galaxies Care about AGB Stars II", held in Vienna, August 16-20, 2010; eds Franz Kerschbaum, Thomas Lebzelter, and Bob Wing, ASP Conf. Serie