Surface Plasmon Resonance Analysis of Aqueous Mercuric Ions

Abstract Mercuric ions, Hg [II], can be detected and quantitated by measuring the surface plasmon resonance (SPR) signal when a gold film treated with 1,6-hexanedithiol (HDT), which produced a self-assembled monolayer, is exposed to mercury ions in aqueous solution. Changes in the SPR signal allowed not only quantification of the concentration of Hg [II] in solution from 1.0 nM to 1.0 mM but also selective detection of Hg [II

Poly(dimethylsiloxane) microfluidic flow cells for surface plasmon resonance spectroscopy

Abstract A fabrication technique has been developed that uses a novolak-based positive photoresist, SPR-220, for fabrication of channels in poly(dimethylsiloxane) (PDMS) that are up to 61 m deep. Like methods relying on SU-8 (an epoxy-based negative photoresist), the new method enables rapid design and fabrication of microfluidic devices but avoids possible incompatibilities with standard cleanroom processes. The utility of devices fabricated with this technique is demonstrated for use with surface plasmon resonance spectroscopy (SPR). Two microfabricated flow cells having volumes of 336 and 73 nl were used to detect an analyte in bulk solution and deposition of proteins on a surface