Antagonism of the complement component C4 by flavivirus nonstructural protein NS1

The complement system plays an essential protective role in the initial defense against many microorganisms. Flavivirus NS1 is a secreted nonstructural glycoprotein that accumulates in blood, is displayed on the surface of infected cells, and has been hypothesized to have immune evasion functions. Herein, we demonstrate that dengue virus (DENV), West Nile virus (WNV), and yellow fever virus (YFV) NS1 attenuate classical and lectin pathway activation by directly interacting with C4. Binding of NS1 to C4 reduced C4b deposition and C3 convertase (C4b2a) activity. Although NS1 bound C4b, it lacked intrinsic cofactor activity to degrade C4b, and did not block C3 convertase formation or accelerate decay of the C3 and C5 convertases. Instead, NS1 enhanced C4 cleavage by recruiting and activating the complement-specific protease C1s. By binding C1s and C4 in a complex, NS1 promotes efficient degradation of C4 to C4b. Through this mechanism, NS1 protects DENV from complement-dependent neutralization in solution. These studies define a novel immune evasion mechanism for restricting complement control of microbial infection

Bluetooth Load-Cell-Based Support-Monitoring System for Safety Management at a Construction Site

At construction sites, temporary facilities have caused continuous collapse accidents, causing damage to human life. If the concrete placing height is high and the worker is pushed into one place at the time of placing, the working load may be exceeded and a collapse accident may occur. In order to solve this problem, in this research, we developed a monitoring load-measurement program based on a Bluetooth wireless load cell (load-cell sensor) so that the load can be converted to digital and the numerical value can be confirmed by the pressure sensor. The load cell using Bluetooth was designed and manufactured according to the support. Then, the performance was verified through 3D finite element analysis by modeling and experimental tests. In addition, we constructed a system to generate notifications and warnings step by step when the load is close to a dangerous load, confirmed the load distribution pattern by position, and established a method to confirm real-time data numerically and graphically. Finally, we evaluated the practical application of the load-monitoring system using field-test data using a wireless load-cell