Exercise Increases Neural Stem Cell Number in a GH-Dependent Manner, Augmenting the Regenerative Response in Aged Mice

The exercise-induced enhancement of learning and memory, and its ability to slow age-related cognitive decline in humans led us to investigate whether running stimulates periventricular (PVR) neural stem cells (NSCs) in aging mice, thereby augmenting the regenerative capacity of the brain. To establish a benchmark of normal aging on endogenous NSCs, we harvested the PVR from serial vibratome sections through the lateral ventricles of juvenile (6-8 weeks), 6, 12, 18, and 24-month-old mice, culturing the cells in the neural colony forming cell assay. A significant decline in NSC frequency was apparent by 6-months (~40%) ultimately resulting in a ~90% reduction by 24-months. Concurrent with this decline was a progressive loss in regenerative capacity, as reflected by an incomplete repopulation of neurosphere-forming cells following gamma cell irradiation-induced depletion of the PVR. However voluntary exercise (i.e. 21 days of running) significantly increased NSC frequency in mic

Some quit, others persist: similarities and disparities of healthcare providers' speech concerning obesity

Background Recent studies indicate that healthcare providers, especially in primary healthcare, have negative beliefs and attitudes towards obese, which are negatively affecting their practices by not taking this issue as seriously as they should and, therefore, compromising the success of obesity treatment. However, data is not conclusive and quantitative research is not being able to clarify how health professionals' practices and roles are affected by the way they perceive obesity and obese people. Method Semi-structured interviews about beliefs, attitudes and practices about obesity were conducted withe Portuguese general practitioners, nutritionists and nurses working in primary health care centers in the north of Portugal. Data was analyzed according to thematic analysis' procedures. Results The main themes indicate that all groups are concerned about the obesity pandemic and have similar negative beliefs and attitudes toward obese, who are described as being unmotivated, noncompliant and demonstrating a passive coping and a lack of understanding about the gravity of their condition. General practitioners, due to patients' lack of compliance and success, feel frustrated, have lower expectations of efficacy and are negative about their role in the treatment, giving up in most of the cases. Nutritionists and nurses demonstrate an active role, are persistent, perceived themselves as being able to positively modify obese motivation and believe in the success of the interventions, which, however, are described as a constant struggle between them and the patients. It seems to exist communication problems between these three groups. Discussion In order to achieve success, healthcare providers should be aware of how their beliefs and attitudes can influence their practices. Education and training concerning treatment options and communications skills should be improved as well as a bigger emphasis should be put on a multidisciplinary approach to obesity.Introdução Investigações recentes no âmbito da obesidade sugerem que as crenças, atitudes e práticas de vários profissionais de saúde, principalmente ao nível dos cuidados de saúde primários, parecem estar a influenciar negativamente o comportamento destes técnicos no tratamento desta doença, não lhe dando a devida importância e contribuindo para a manutenção das taxas de obesidade. As críticas têm apontado para a primazia de investigações quantitativas e para a ausência de estudos comparativos com diferentes grupos de profissionais de saúde. Método Neste estudo foram realizadas entrevistas semiestruturadas a médicos de família, nutricionistas e enfermeiros, a laborar em centros de saúde dos distritos de Braga, Porto e Aveiro. As entrevistas foram transcritas e analisadas, segundo os princípios da análise temática. Resultados Os 3 grupos apresentam crenças e atitudes negativas em relação aos obesos, que são descritos como desmotivados e passivos face ao tratamento, não aderindo na maioria das vezes, visto desvalorizarem a obesidade enquanto problema de saúde. Os médicos de família possuem baixas expectativas de sucesso, sentindo‐se frustrados com a falta de adesão, o que os leva a adotar uma postura passiva e resignada face ao tratamento. Os nutricionistas e enfermeiros percecionam‐se como agentes ativos, considerando‐se capazes de influenciar a motivação dos obesos; acreditam no seu sucesso, mas descrevem o processo como uma luta constante. Há várias referências a problemas de comunicação entre os 3 grupos de profissionais. Discussão Para uma maior eficácia no tratamento da obesidade torna‐se peremptório alertar os profissionais de saúde para o impacto que as suas crenças poderão exercer na prática, reforçar a abordagem multidisciplinar e promover o aumento dos conhecimentos e de opções de tratamento, e a melhoria da comunicação entre os vários profissionais(undefined)info:eu-repo/semantics/publishedVersio

From scandal to monastic penance: a reconciliatory manuscript from the early twelfth-century abbey of St. Laurent in Liège

An important element of monastic penance and conflict resolution was its repetitive, almost cyclical nature. The manuscripts that were used during these performances often proceed implicitly, which makes them difficult to contextualize and understand. This article considers a possible example of such "hidden" reconciliatory discourse in a manuscript that was produced for the congregation of St. Laurent in Liege around the turn of the eleventh century: Brussels, Royal Library 9361-9367. It examines the sin of pride in monastic dignitaries, discusses the best way to atone for it, and provides tools for the penitent to start living a more virtuous life in the future. The surviving evidence suggests that this manuscript was produced in reaction to the deeds of abbot Berenger, whose actions in 1095 were considered scandalous by contemporaries because he had led his monks into confusion and sin. The article shows how the combination of texts in this manuscript takes on a different meaning because of these politically charged circumstances, and argues that the St. Laurent manuscript was a discreet but methodical way to end the resulting estrangement between Berenger and his monks. In this interpretation, Brussels RL 9361-9367 is a rare and highly relevant testimony to the ways in which monks in the early twelfth century dealt with psychological and social tensions in the wake of an intra-group conflict

Computational fluid dynamics modelling in cardiovascular medicine

This paper reviews the methods, benefits and challenges associated with the adoption and translation of computational fluid dynamics (CFD) modelling within cardiovascular medicine. CFD, a specialist area of mathematics and a branch of fluid mechanics, is used routinely in a diverse range of safety-critical engineering systems, which increasingly is being applied to the cardiovascular system. By facilitating rapid, economical, low-risk prototyping, CFD modelling has already revolutionised research and development of devices such as stents, valve prostheses, and ventricular assist devices. Combined with cardiovascular imaging, CFD simulation enables detailed characterisation of complex physiological pressure and flow fields and the computation of metrics which cannot be directly measured, for example, wall shear stress. CFD models are now being translated into clinical tools for physicians to use across the spectrum of coronary, valvular, congenital, myocardial and peripheral vascular diseases. CFD modelling is apposite for minimally-invasive patient assessment. Patient-specific (incorporating data unique to the individual) and multi-scale (combining models of different length-And time-scales) modelling enables individualised risk prediction and virtual treatment planning. This represents a significant departure from traditional dependence upon registry-based, populationaveraged data. Model integration is progressively moving towards 'digital patient' or 'virtual physiological human' representations. When combined with population-scale numerical models, these models have the potential to reduce the cost, time and risk associated with clinical trials. The adoption of CFD modelling signals a new era in cardiovascular medicine. While potentially highly beneficial, a number of academic and commercial groups are addressing the associated methodological, regulatory, education-And service-related challenges

A qualitative study of GPs' views towards obesity : are they fighting or giving up?

Objectives: Several studies indicate that general practitioners (GPs) are not taking the issue of obesity as seriously as they should. Therefore, the aim of this study was to understand GPs' views about obesity and obese people and how these professionals perceive their role in the treatment of this disease. Study design: Qualitative study using semi-structured interviews. Methods: Sixteen semi-structured interviews were conducted with Portuguese GPs. Data were analyzed according to thematic analysis procedures. Results: GPs are negative about their own role in obesity treatment. Although they believe it is part of their job to advise obese patients on the health risks of obesity, the majority of doctors think they are not making any difference in getting their patients to make long term lifestyle changes. GPs hold negative attitudes towards these patients blaming them for being unmotivated and non-compliant and are also pessimistic about their ability to lose weight. Doctors are facing a dilemma in their practices: they want to play an active role but, due to a set of negative beliefs and perceived barriers, they are playing a relatively passive role, feeling defeated and unmotivated, which is reflected in a decrease of efforts and a willing to give up on most of the cases. Conclusions: This issue should be taken in to account during physicians' education since doctors should be aware of how their own beliefs and attitudes influence their behaviour and practices, compromising, therefore, the adherence to and the success in obesity treatment. They seem to need more precise guidelines and better tools for screening and management of obesity, more referral options, and improved coordination with other specialitie

Behavioural economics, motivating psycho-education improvements; a mobile technology initiative in South Africa

Here we report on a health behavioural support project, using incentivised behaviour on a mobile platform through M4JAM. This was a proof of concept study to support further developments, more specifically targeted at the management of Tuberculosis and Human Immunodeficiency Virus. The study reported here examines the impact of financial rewards and app towards improving mental health outcomes in South Africa. 136 participants were recruited from a database and dichotomized into self-determined and heteronomous groups based on self-report scores. Overall the findings highlighted that personal financial incentives have a role in motivating behaviour and that individuals with higher levels of self-determinate motivation. The findings are discussed in light of the usefulness of an incentivized mobile platform in real-world practice to encourage mental health improvements in a low to middle-income countries

Cellular Radiosensitivity: How much better do we understand it?

Purpose: Ionizing radiation exposure gives rise to a variety of lesions in DNA that result in genetic instability and potentially tumorigenesis or cell death. Radiation extends its effects on DNA by direct interaction or by radiolysis of H2O that generates free radicals or aqueous electrons capable of interacting with and causing indirect damage to DNA. While the various lesions arising in DNA after radiation exposure can contribute to the mutagenising effects of this agent, the potentially most damaging lesion is the DNA double strand break (DSB) that contributes to genome instability and/or cell death. Thus in many cases failure to recognise and/or repair this lesion determines the radiosensitivity status of the cell. DNA repair mechanisms including homologous recombination (HR) and non-homologous end-joining (NHEJ) have evolved to protect cells against DNA DSB. Mutations in proteins that constitute these repair pathways are characterised by radiosensitivity and genome instability. Defects in a number of these proteins also give rise to genetic disorders that feature not only genetic instability but also immunodeficiency, cancer predisposition, neurodegeneration and other pathologies. Conclusions: In the past fifty years our understanding of the cellular response to radiation damage has advanced enormously with insight being gained from a wide range of approaches extending from more basic early studies to the sophisticated approaches used today. In this review we discuss our current understanding of the impact of radiation on the cell and the organism gained from the array of past and present studies and attempt to provide an explanation for what it is that determines the response to radiation