Comparative proteomic analysis reveals alterations in development and photosynthesis-related proteins in diploid and triploid rice

Six pairs of primers were designed for gene-specific transcript amplification. (DOC 25 kb

Comparative proteomics analysis of proteins expressed in the I-1 and I-2 internodes of strawberry stolons

Abstract Background Strawberries (Fragaria ananassa) reproduce asexually through stolons, which have strong tendencies to form adventitious roots at their second node. Understanding how the development of the proximal (I-1) and distal (I-2) internodes of stolons differ should facilitate nursery cultivation of strawberries. Results Herein, we compared the proteomic profiles of the strawberry stolon I-1 and I-2 internodes. Proteins extracted from the internodes were separated by two-dimensional gel electrophoresis, and 164 I-1 protein spots and 200 I-2 protein spots were examined further. Using mass spectrometry and database searches, 38 I-1 and 52 I-2 proteins were identified and categorized (8 and 10 groups, respectively) according to their cellular compartmentalization and functionality. Many of the identified proteins are enzymes necessary for carbohydrate metabolism and photosynthesis. Furthermore, identification of proteins that interact revealed that many of the I-2 proteins form a dynamic network during development. Finally, given our results, we present a mechanistic scheme for adventitious root formation of new clonal plants at the second node. Conclusions Comparative proteomic analysis of I-1 and I-2 proteins revealed that the ubiquitin-proteasome pathway and sugar-hormone pathways might be important during adventitious root formation at the second node of new clonal plants.</p

Transcriptome and Metabolome Integrated Analysis of Two Ecotypes of Tetrastigma hemsleyanum Reveals Candidate Genes Involved in Chlorogenic Acid Accumulation

T. hemsleyanum plants with different geographical origins contain enormous genetic variability, which causes different composition and content of flavonoids. In this research, integrated analysis of transcriptome and metabolome were performed in two ecotypes of T. hemsleyanum. There were 5428 different expressed transcripts and 236 differentially accumulated metabolites, phenylpropane and flavonoid biosynthesis were most predominantly enriched. A regulatory network of 9 transcripts and 11 compounds up-regulated in RG was formed, and chlorogenic acid was a core component

A Study of Stray Neutron Field Measurements for the Neutron Scattering Instruments at CSNS

Stray neutrons might cause several negative impacts. However, it is usually difficult to conduct precise stray neutron simulations using the Monte Carlo method. Therefore, in this study, a measurement technique was proposed to study the stray neutrons experimentally inside the neutron scattering instruments at China Spallation Neutron Source (CSNS). The adopted measurement instruments comprise an extended-range Bonner sphere spectrometer and a commercial neutron ambient-dose-equivalent dosimeter, which enables us to directly measure the neutron spectra and ambient-dose equivalent H*(10) values. Verification experiments were performed inside the BL06 beam line experimental area at CSNS at two exposed locations with different sample conditions. Comparison of the experimentally measured neutron spectra, integral neutron fluence, and H*(10) value with the simulations demonstrated the feasibility of using the proposed method for studying stray neutrons for the neutron instruments

A Study of Stray Neutron Field Measurements for the Neutron Scattering Instruments at CSNS

Stray neutrons might cause several negative impacts. However, it is usually difficult to conduct precise stray neutron simulations using the Monte Carlo method. Therefore, in this study, a measurement technique was proposed to study the stray neutrons experimentally inside the neutron scattering instruments at China Spallation Neutron Source (CSNS). The adopted measurement instruments comprise an extended-range Bonner sphere spectrometer and a commercial neutron ambient-dose-equivalent dosimeter, which enables us to directly measure the neutron spectra and ambient-dose equivalent H*(10) values. Verification experiments were performed inside the BL06 beam line experimental area at CSNS at two exposed locations with different sample conditions. Comparison of the experimentally measured neutron spectra, integral neutron fluence, and H*(10) value with the simulations demonstrated the feasibility of using the proposed method for studying stray neutrons for the neutron instruments

1/1.5 μm dual-band high-energy square-wave mode-locked fiber laser based on Er/Yb-codoped double cladding fiber

A 1 μm/1.5 μm dual-band simultaneously mode-locked fiber laser with high-energy square-wave pulses has been demonstrated based on a common Erbium/ Ytterbium-codoped double cladding fiber.</p