Construction and Performance Research of Reinforced Iron-Based Powder Metallurgy Materials Based on Phyllanthin as Drug Transport Carriers

Iron-based powder metallurgy materials are the largest type of powder metallurgy materials, mainly used in structural parts, bearings, and friction materials. Iron-based powder metallurgy materials have a series of advantages such as low cost, good machinability, good weldability, and heat treatment. In recent years, the enhanced iron-based powder metallurgy materials based on lavender elements have become a hot spot in the development of material transportation carriers. In order to study the effects of different hot pressing and sintering temperatures on the density, microstructure, and hardness of the enhanced iron-based powders of caladium, we conducted related studies on the structure core properties of the enhanced iron-based powders of caladium to explore whether it can be used as a drug transport carrier. The research results show that hot pressing sintering can make the powder achieve high densification at lower temperature and shorter cycle, especially in the preparation of difficult-to-form and sintered powder metallurgy materials with unique advantages

The Neuroprotective Effects of Astragaloside IV against H2O2-Induced Damage in SH-SY5Y Cells are Associated with Synaptic Plasticity

The aim of this study was to investigate whether the neuroprotective effects of astragaloside IV (AS-IV) against hydrogen peroxide (H2O2)-induced damage on human neuroblastoma cell line (SH-SY5Y) are associated with synaptic plasticity. The concentration screening of AS-IV and H2O2 on SH-SY5Y cells and the protective effects of AS-IV on SH-SY5Y cells under H2O2 stress were all determined by MTT assay. The expression of postsynaptic density 95 (PSD-95) and growth-associated protein 43 (GAP-43) were measured by western blot (WB) and inmunofluorescence staining assay under the same treatment conditions. According to the MTT results, the concentration of H2O2 at 50 μmol/L for 3 h was used for the cell damage model, and various concentrations of AS-IV (0.1, 0.2, 0.3, and 0.4 μmol/L) were used to affect SH-SY5Y cells. The MTT results showed that pretreatment of SH-SY5Y cells with AS-IV (0.1, 0.2, 0.3, and 0.4 μmol/L) attenuated the damage induced by H2O2 (50 μmol/L, 51.62% cell viability) and increased cell viability to 64.19, 63.48, 65.86, and 65.81%, respectively. Western blot analysis and immunofluorescence staining showed that the protective effects of AS-IV against SH-SY5Y cell damage caused by H2O2 resulted in reduced expression of PSD-95 and increased expression of GAP-43 in comparison with the H2O2 treatment group. The conclusion shows that AS-IV protected SH-SY5Y cells and enhanced their viability under H2O2 stress. AS-IV may facilitate presynaptic and postsynaptic plasticity to exert protective effects against oxidative damage of SH-SY5Y cells

Study on the culture of Escherichia coli with different hydrolysis depth of globin

Objective: To explore the effect of culture medium made of peptide fragments of different sizes of globin hydrolyzed by protease on the culture of Escherichia coli. Method: with fresh pig blood as raw material, hydrolyze pig blood protein under fixed conditions, and screen out the enzyme with the highest degree of hydrolysis. Then conduct orthogonal test on the enzyme to determine the best hydrolysis conditions; the beads were obtained under the best hydrolysis conditions. The Escherichia coli was cultured on different peptone culture bases that were prepared by hydrolysate going through different pore size ultrafiltration membrane samples. The culture of Escherichia coli at different hydrolysis depths is determined by detecting the total amount with Escherichia coli detection method. Results: Trypsin has the best hydrolysis effect. The best hydrolysis conditions are as follows: enzyme dosage of 0.2%, hydrolysis time of 12h, temperature of 50°C, initial pH of 7.5 and mass fraction of substrate protein of 8%. Through growth curves of Escherichia coli, it is known that the smaller the pore size of the ultrafiltration membrane, the smaller the molecular weight of the peptide fragment, and the greater the density of the Escherichia coli bacterial solution. Conclusion: Through analyzing the production curves of Escherichia coli in different culture medium, it is concluded that the smaller the molecular weight of the protein peptides, the higher the density of Escherichia coli solution in the LB liquid medium prepared by it, and the better the Escherichia coli culture effect

Research on the synthesis of chitosan@MIL-100(Fe)

Objective: Studying new carrier materials to solve the problems of bioavailability and side effects of drugs in the body and choose the suitable carrier to coat the drug. This carrier not only improves the release and absorption of drug molecules in the body, but also significantly improves the bioavailability and reduces the toxic side effects of high concentrated drugs. Therefore, this paper focuses on the research of the synthesis of a new carrier material. Methods:Using ferric nitrate as iron source, first add ferric nitrate, 1,3,5-benzene tricarbonic acid, chitosan solution and deionized water pro rata, then synthesize the product using mechanical stirring, and purify and dry the product. Results:The maximum yield of chitosan is obtained when the concentration of chitosan is 1% using mechanical stirring method, and the yield is 0.6330 g. Conclusion:The results show that the thinner the concentration of chitosan solution is, the better synthesizes the chitosan@MIL-100(Fe) and the higher the yield is. And it reaches the best result when the concentration is 1%

Study on Extraction Technology of Pitaya Polysaccharide

Using pitaya as raw material, this paper used ethanol subsiding method to study the extraction technology of pitaya polysaccharide by single factor test and orthogonal test. Through orthogonal test, we have found the optimum extraction conditions of pitaya polysaccharide as follows: extraction temperature 60℃, solid-liquid ratio 1:30, extraction time 3h. Under the optimal extraction conditions, the yield of pitaya polysaccharide was 1.13%

Nonequilibrium charge carrier lifetime testing equipment for semiconductor materials by a contactless microwave phase method

Conference Name:2012 3rd International Conference on Advances in Materials and Manufacturing Processes, ICAMMP 2012. Conference Address: Beihai, China. Time:December 22, 2012 - December 23, 2012.University of Wollongong, Australia; Northeastern University, China; University of Science and Technology Beijing; Hong Kong Industrial Technology Research CentreThe accurate measurement of nonequilibrium charge carrier lifetime is of vital significance in research and manufacture of crystalline silicon solar cells. A testing equipment based on a contactless microwave phase method was implemented by being embedded with GPIB, FPGA and a lock-in analyzer. A friendly operation interface was developed, based on the graphic programming language LabVIEW. The virtue of the equipment is achieved by automatic data acquisition and processing, which improves the automatization, efficiency and accuracy of the measurement. ? (2013) Trans Tech Publications, Switzerland

Study on Aqueous Enzymatic Extraction Process of Common Pistache Oil

The aqueous enzymatic method was applied to extract the common pistache oil and the optimal extraction process conditions were identified. By observing the effect of enzymatic hydrolysis time, pH value, temperature on aqueous enzymatic extraction process and performing the orthogonal experiment based on the single factor test, the optimal process parameters were obtained, namely, the optimal time, temperature, and pH value were respectively 3 h, 50℃, and 7 with a final extraction rate of 25.38 %

Comparison of Total Flavonoids Content in Different Bamboo Shoots and Different Parts

The paper determined the content of total flavonoids in different types and parts of rhizome shoot and winter bamboo shoots by UV spectrophotometry for comparison. The results showed that the bamboo shoot sheath had a higher level of total flavonoids than in the bamboo shoots, and the rhizome shoot had a higher level of total flavonoids than the winter bamboo shoots in different parts

A Study on the Mechanism of Action of Galangal in the Treatment of Gastric Cancer Using Network Pharmacology Technology

To study the mechanism of galangal in the treatment of gastric cancer by network pharmacology. The TCMSP database was used to collect the effective compounds and potential targets of galangal, and the genes associated with gastric cancer were obtained through the GeneCards database, and Venn obtained the interaction genes of the effective compound targets of galangal and gastric cancer targets, plotted the interaction genes into PPI networks, and screened out key targets. The interacting genes were imported into Metascape database for GO enrichment analysis and KEGG signal enrichment. A total of 13 active compounds and 207 potential downstream target genes were screened by TCMSP database. Have 5222 gastric cancer target genes through GeneCards database, there were a total of 150 interactive genes and 6 key genes: TP53, AKT1, JUN, HSP90AA1, IL6, and CASP3. These interacting genes involved 30 typical GO entries and 20 KEGG signals. Galangal may play a role in the treatment of gastric cancer by means of multi-component, multi-target and multi-signal pathway

Study on the Antilipidemic Activity of Artemisia Annua Aqueous Extract

Artemisia annua is an annual herb, which is a traditional Chinese medicine in China. The high water soluble artemisinin derivative extracted from Artemisia annua is Artemisia annua water extract. In this experiment, the modeling method is used, and the Artemisia annua water extract is used alone or in combination with other lipid-lowering drugs. Finally, the results showed that the effect of the combination of drugs was better than that of Artemisia annua water extract alone, and the effect of the drug ratio of 1:1 was the best. It can significantly reduce the content of triglyceride in serum and cholesterol, increase the ratio of H/L, and show a good synergistic effect