Transcriptional activator gene based phylogenetic analysis of dolichos yellow mosaic virus infecting lablab bean (Dolichos lablabL.)

Lablab bean (Dolichos lablab L.) is one the important crop, cultivated as vegetable, pulses as it is rich in protein. It is affected from viral disease i.e. dolichos yellow mosaic virus. The causative agent is begomovirus belongs to geminivirus family. Begomovirus contain bipartite genome having two type of DNA-A and DNA-B. DNA- A helps in replication and DNA-B helps in movement. These DNA have six different type of gene coat protein gene, transcriptional activator gene, replica-tion associated gene, replication enhancer gene, pre coat protein involved in different function associated with it. The present investigation was carried out to investigate the transcriptional activa-tor gene based phylogenetic analysis of Dolichos yellow mosaic virus infecting D. lablab. This study is based on the transcriptional activator gene which is used in transactivation of genes, contains three conserved domains: a basic domain at N-Terminus, a central DNA binding domain and activator domain. The genome databases of dolichos yellow mosaic virus were taken from NCBI site total six genome was available and were used with Clustal W and CLC BIO were the bioinformatic tools for determining sequence homology among genome present in different geographical location. The absence of functional specificity suggests that all begomovirus contains a common element interacts with cellular proteins of other viruses reveals the phylogenetic analysis with the other species Dolichos in the different geographical location

Physiological comparison of root trainer and polybag plants of Hevea brasiliensis

The physiology of   root trainer plants in comparison to polybag plants of two popular Hevea clones,   RRII 105 and RRII 430 was studied. Dry matter partitioning and physiological   parameters like leaf water potential, relative water content, chlorophyll   content, PS II activity and photosynthetic efficiency were studied in root   trainer and polybag plants and compared. Root trainer plants had only 55 per   cent less biomass than polybag plants at two whorled stage. No variation was   noticed in water relations among the poly bag and root trainer plants. Total   chlorophyll content and chlorophyll a/b ratio were higher in polybag   plants than root trainer plants of both clones. Polybag plants showed higher   light saturation point when gas exchange was measured at different   photosynthetic photon flux densities. Though there was no variation in   effective quantum yield (Φ PSII), the root trainer plants of RRII 105 showed   more excess electrons (J*) at higher light intensities. Photosystem II   activity and photosynthetic carbon assimilation rates were less in root   trainer plants under open and shaded conditions. The results indicated that   the root confinement in root trainer plants has significant effect on the   physiology of plants. The reduction in plant biomass and other physiological   traits in root trainer plants can be attributed to the limited space in the   container, limiting further root growth, which otherwise is beneficial for   giving a priming effect as long as the plant remains in the container.   Further studies are needed to quantify this ‘stress factor’ in root trainer   plants and its implications in growth and establishment of plants in the   field