95 research outputs found
Differentiation of peanut clump virus serotypes by monoclonal antibodies
Des anticorps monoclonaux dirigĂ©s contre le virus du "clump" de l'arachide ont permis de caractĂ©riser 5 sĂ©rotypes du virus. La comparaison de 4 types de tests immunoenzymatiques ELISA a permis de sĂ©lectionner celui qui est le mieux adaptĂ© au diagnostic de routine et Ă la diffĂ©renciation entre sĂ©rotypes du virus. La plupart des anticorps monoclonaux conservent leur activitĂ© aprĂšs adsorption sur la phase solide en ELISA, et le mĂȘme anticorps peut ĂȘtre utilisĂ© comme capteur et comme anticorps biotinylĂ©. Etant donnĂ© que les anticorps obtenus sont capables de reconnaĂźtre des changements mineurs de conformation de l'antigĂšne viral, il est important de sĂ©lectionner avec soin le format du test ELISA utilisĂ© dans les comparaisons entre diffĂ©rents isolats du virus. (RĂ©sumĂ© d'auteur
Transduction of SIV-Specific TCR Genes into Rhesus Macaque CD8+ T Cells Conveys the Ability to Suppress SIV Replication
The SIV/rhesus macaque model for HIV/AIDS is a powerful system for examining the contribution of T cells in the control of AIDS viruses. To better our understanding of CD8(+) T-cell control of SIV replication in CD4(+) T cells, we asked whether TCRs isolated from rhesus macaque CD8(+) T-cell clones that exhibited varying abilities to suppress SIV replication could convey their suppressive properties to CD8(+) T cells obtained from an uninfected/unvaccinated animal.We transferred SIV-specific TCR genes isolated from rhesus macaque CD8(+) T-cell clones with varying abilities to suppress SIV replication in vitro into CD8(+) T cells obtained from an uninfected animal by retroviral transduction. After sorting and expansion, transduced CD8(+) T-cell lines were obtained that specifically bound their cognate SIV tetramer. These cell lines displayed appropriate effector function and specificity, expressing intracellular IFNÎł upon peptide stimulation. Importantly, the SIV suppression properties of the transduced cell lines mirrored those of the original TCR donor clones: cell lines expressing TCRs transferred from highly suppressive clones effectively reduced wild-type SIV replication, while expression of a non-suppressing TCR failed to reduce the spread of virus. However, all TCRs were able to suppress the replication of an SIV mutant that did not downregulate MHC-I, recapitulating the properties of their donor clones.Our results show that antigen-specific SIV suppression can be transferred between allogenic T cells simply by TCR gene transfer. This advance provides a platform for examining the contributions of TCRs versus the intrinsic effector characteristics of T-cell clones in virus suppression. Additionally, this approach can be applied to develop non-human primate models to evaluate adoptive T-cell transfer therapy for AIDS and other diseases
The long zinc finger domain of PRDM9 forms a highly stable and long-lived complex with its DNA recognition sequence
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