Overview of PCR-based and DNA sequencing results.

*<p>positivity criteria in analogy to the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0002163#pntd.0002163-Bustin1" target="_blank">[19]</a>. GenBank accession numbers:</p>1<p>BankIt1587796 Seq2 KC283067.</p>2<p>BankIt1587796 Seq3 KC283068.</p>3<p>BankIt1587796 Seq1 KC283066.</p

Reports of apparent mixed infections in Asia that included rickettsioses.

<p>Reports of apparent mixed infections in Asia that included rickettsioses.</p

Results of RDT stored at 35°C and in the hut in comparison to RDT stored at 4°C.

<p>Results of RDT stored at 35°C and in the hut in comparison to RDT stored at 4°C.</p

Efficiency of dengue RNA extraction from RDT-S part (7mm), RDT-WS part (15 mm) and FP (2 disc of 6mm) compared to the direct extraction for all isolate dilutions for the four dengue serotypes.

<p>In y axis: RNA recovery in percentage: (RDTor FP/Dir)*100 = ratio of the mean number of dengue RNA copies/μl recovered by RDT(S part or WS: whole S part) or FP extraction over the mean number of dengue RNA copies/μl recovered by the direct extraction. X axis: RSD = relative standard deviation for the different extraction techniques.</p

Mean number of dengue RNA copies/μl recovered by extraction from the 4 parts of RDT strip compared to the direct extraction.

<p>Mean number of dengue RNA copies/μl recovered by extraction from the 4 parts of RDT strip compared to the direct extraction.</p

NS1 RDT strip cutting.

<p>A: NS1 cassette. B: strip outside the cassette with the location of the 4 parts cut for the experiment, N: nitrocellulose, C: conjugate pad, S: sample pad, N1 and N2: nitrocellulose membrane. C: strip with the location of the whole S pad (WS) cut for the experiment.</p

Comparison of agreements of the different techniques for RNA preparation with the neat serum extraction for Mahosot Hospital samples.

<p>Comparison of agreements of the different techniques for RNA preparation with the neat serum extraction for Mahosot Hospital samples.</p

Number of patients found positive by DENV.

<p>All RT-PCR performed after extraction from RDT or filter paper and from neat serum for 99 patients admitted at Mahosot Hospital.</p

Number of patients positive by DENV.

<p>All RT-PCR performed after extraction from RDT or filter paper and from neat serum for 362 patients admitted at Salavan Provincial Hospital.</p

Mean number of dengue RNA copies/μl recovered by extraction from RDT S, WS parts and filter paper, compared to direct extraction.

<p>Mean number of dengue RNA copies/μl recovered by extraction from RDT S, WS parts and filter paper, compared to direct extraction.</p