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Not AvailableRubus ellipticus Smith. commonly known as ‘Yellow Himalayan raspberry’ is an important member of Rosaceae family with high medicinal importance having antioxidant and antibacterial properties. This study was done with the objective of characterizing 21 R. ellipticus collections from different regions of India, based on morphological descriptors and expressed sequence tags-simple sequence repeat (EST-SSR) markers. Broad phenotypic variability among the R. ellipticus collections was detected by using morphological descriptors. The Euclidian distance matrix discriminated two groups, based on morphological characters. A set of 68 EST-SSR markers were developed based on 7777 unigenes generated by us from ‘Kumarhatti-1’ collection of R. ellipticus using assembled pair—end sequences from Illumina NextSeq 500. The ESTs containing simple sequence repeat motifs were extracted using Microsatellite identification tool and primers were designed. Out of 68 EST-SSRs polymorphism was detected using 61 (89.7%) primer pairs, indicating high genetic variability in the R. ellipticus collections. Effective multiplex ratio, marker index and polymorphism information content were 0.43, 0.24 and 0.50, respectively. The unweighted pair group method with arithmetic mean coincided with the bootstrap analysis and two groups were formed, separating the collections Uttrakhand from the other. Morphological descriptors along with EST-SSR markers proved efficient in detecting the levels of genetic variability among the collections maintained in the field. These results can be used as an additional source of exploitation in R. ellipticus breeding programs.Not Availabl

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Not AvailableA study was conducted on the combining ability analysis in a 11 ×11 half-diallel cross of garden pea (Pisum sativum L.). F1 hybrids (55) along with 11 parental lines were grown in randomized block design with three replications. The mean square due to GCA and SCA were highly significant for all the characters indicating the importance of additive and non-additive genetic components for the characters under study. Further, σ2s was more than σ2g for all the characters suggested the importance of non-additive variance for the improvement of these characters. The parents P2 (GP-17), P5 (GP-473) and P7 (VRP-7) were observed to be good combiners for most characters under study including yield per plant. The crosses P6 × P10, P2 × P8 and P8 × P10 were most promising combinations for total yield per plant. The results indicated the importance of recombinant breeding for effective utilization of non-additive genetic variance in garden pea.Not Availabl

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Not AvailableA study of fifty five F1 hybrids of garden pea (Pisum sativum L.) in a half diallel set involving eleven parents were conducted in a Randomized Block Design with three replications. Over-dominance was found for all the characters which indicated predominance of non-additive gene action for the inheritance of these characters. The positive value of F in all the characters showed preponderance of dominant alleles in parents. Dominance effect (H) was found to be higher than additive effect (D) for all characters except plant height & days to 50% flowering and average degree of dominance was more than one for all the characters, confirmed the predominant role of non-additive gene action. For all traits narrow sense of heritability was less than 50% except plant height and days to 50% flowering, which suggests the preponderance of non-additive gene action and commercial exploitation of recombinant breeding for improvement of these characters.Not Availabl

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Not AvailableClusterbean (Cyamopsis tetragonoloba (L.) Taub.), also known as Guar is a widely cultivated dryland legume of Western India and parts of Africa. Apart from being a vegetable crop, it is also an abundant source of a natural hetero-polysaccharide called guar gum or galactomannan which is widely used in cosmetics, pharmaceuticals, food processing, shale gas drilling etc. Here, for the first time we are reporting a chromosome-scale eference genome assembly of clusterbean, from a high galactomannan containing popular guar cultivar, RGC-936, by combining sequenced reads from Illumina, 10x Chromium and Oxford Nanopore technologies. The initial assembly of 1580 scaffolds with an N50 value of 7.12 Mbp was generated. Then, the final genome assembly was obtained by anchoring these scaffolds to a high density SNP map. Finally, a genome assembly of 550.31 Mbp was obtained in 7 pseudomolecules corresponding to 7 chromosomes with a very high N50 of 78.27 Mbp. We finally predicted 34,680 protein-coding genes in the guar genome. The high-quality chromosome-scale cluster bean genome assembly will facilitate understanding of the molecular basis of galactomannan biosynthesis and aid in genomics-assisted breeding of superior cultivars.Not Availabl