1 research outputs found
ΠΡΡΠΎΡΠΈΠ°ΡΠΈΠΈ ΠΎΠΆΠΈΡΠ΅Π½ΠΈΡ Ρ ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠ΅Π½Π½ΠΎΡΡΡΡ Π²ΠΈΡΠ°ΠΌΠΈΠ½ΠΎΠΌ D Π² Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΎΡ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΠΎΠ² rs2228570 Π³Π΅Π½Π° VDR ΠΈ rs9939609 Π³Π΅Π½Π° FTO Ρ ΠΆΠΈΡΠ΅Π»Π΅ΠΉ ΡΡΠ΅Π΄Π½Π΅ΠΉ ΠΏΠΎΠ»ΠΎΡΡ ΠΈ ΠΡΠ°ΠΉΠ½Π΅Π³ΠΎ Π‘Π΅Π²Π΅ΡΠ° Π ΠΎΡΡΠΈΠΈ
Background: It has been shown that vitaminΒ D availability depends on the body mass index (BMI). Genetic polymorphisms contribute to the development of vitaminΒ D deficiency.Aim: To study the availability of vitamin D in the population of various regions of the Russian Federation, depending on the BMI values and the rs2228570 polymorphisms of the VDR gene and rs9939609 of the FTO gene.Materials and methods: The rs2228570 polymorphisms of the VDR gene and rs9939609 of the FTO gene were identified in 311 subjects (136, from the midland of Russia, and 175 from the Far North). Serum 25-hydroxyvitaminΒ D [25(OH)D] levels were measured by an immunoenzyme assay in the autumn and winter seasons. Genotyping was performed with the allele-specific amplification and real-time detection of results using TaqMan probes complementary to the polymorphic DNA segments and the CFX96 Real Time System amplifier (Bio-Rad, USA). We studied associations of the rs9939609 polymorphism of the fat mass and obesity-associated (FTO) gene located at 16q12.2, as well as the rs2228570 polymorphism of the vitamin D receptor gene (VDR) located at 12q13.11.Results: Frank vitamin D deficiency (serum 25 (OH) D level20 ng/ml) was observed in 39.7% (54/136) of the sample from the midland, in 40% (14/35) of the migrants and in 30.7% (43/140) of the indigenous inhabitants of the Far North (Nenets). Obese residents of the midland Russia had significantly lower serum 25(OH)D levels, and the indigenous population of the Far North had significantly higher levels than those with BMI30 (Ρ0.05). In the indigenous population of the Northern Region, there was aΒ significant association between vitaminΒ D deficiency and CΒ allele of the rs2228570 polymorphism of the VDR gene (odds ratio [OR] 2.5, 95% confidence interval [CI] 1.46β4.27, p=0.0006) and the AA genotype of the rs9939609 polymorphism of the FTO gene (OR 8.83, 95% CI 0.94β82.5, p=0.02).Conclusion: The association between obesity and vitaminΒ D availability in the individuals with the rs2228570 polymorphism of the VDR gene and the rs9939609 polymorphism of the FTO gene depends on their ethnicity.Β ΠΠΊΡΡΠ°Π»ΡΠ½ΠΎΡΡΡ. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠ΅Π½Π½ΠΎΡΡΡ Π²ΠΈΡΠ°ΠΌΠΈΠ½ΠΎΠΌΒ D Π·Π°Π²ΠΈΡΠΈΡ ΠΎΡ Π²Π΅Π»ΠΈΡΠΈΠ½Ρ ΠΈΠ½Π΄Π΅ΠΊΡΠ° ΠΌΠ°ΡΡΡ ΡΠ΅Π»Π° (ΠΠΠ’). ΠΠΊΠ»Π°Π΄ Π²Β ΡΠ°Π·Π²ΠΈΡΠΈΠ΅ Π΄Π΅ΡΠΈΡΠΈΡΠ° Π²ΠΈΡΠ°ΠΌΠΈΠ½Π° D Π²Π½ΠΎΡΡΡ Π³Π΅Π½Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΡ.Π¦Π΅Π»Ρ β ΠΈΠ·ΡΡΠΈΡΡ ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠ΅Π½Π½ΠΎΡΡΡ Π²ΠΈΡΠ°ΠΌΠΈΠ½ΠΎΠΌ D Π½Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΡΠ°Π·Π½ΡΡ
ΡΠ΅Π³ΠΈΠΎΠ½ΠΎΠ² Π ΠΎΡΡΠΈΠΉΡΠΊΠΎΠΉ Π€Π΅Π΄Π΅ΡΠ°ΡΠΈΠΈ Π²Β Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΎΡ Π²Π΅Π»ΠΈΡΠΈΠ½Ρ ΠΠΠ’ ΠΈΒ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΠΎΠ² rs2228570 Π³Π΅Π½Π° VDR ΠΈΒ rs9939609 Π³Π΅Π½Π° FTO.ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈΒ ΠΌΠ΅ΡΠΎΠ΄Ρ. ΠΠ΄Π΅Π½ΡΠΈΡΠΈΠΊΠ°ΡΠΈΡ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΠΎΠ² rs2228570 Π³Π΅Π½Π° VDR ΠΈΒ rs9939609 Π³Π΅Π½Π° FTO ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Π° ΡΒ 311 ΡΠ΅Π»ΠΎΠ²Π΅ΠΊ (136 ΠΏΡΠΎΠΆΠΈΠ²Π°Π»ΠΈ Π²Β ΡΡΠ΅Π΄Π½Π΅ΠΉ ΠΏΠΎΠ»ΠΎΡΠ΅ Π ΠΎΡΡΠΈΠΈ, 175Β β Π½Π° ΠΡΠ°ΠΉΠ½Π΅ΠΌ Π‘Π΅Π²Π΅ΡΠ΅). ΠΠΎΠ½ΡΠ΅Π½ΡΡΠ°ΡΠΈΡ Π²Β ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ 25-Π³ΠΈΠ΄ΡΠΎΠΊΡΠΈΠ²ΠΈΡΠ°ΠΌΠΈΠ½Π°Β D (25(ΠΠ)D) ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ ΠΈΠΌΠΌΡΠ½ΠΎΡΠ΅ΡΠΌΠ΅Π½ΡΠ½ΡΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Π²Β ΠΎΡΠ΅Π½Π½Π΅-Π·ΠΈΠΌΠ½ΠΈΠΉ ΠΏΠ΅ΡΠΈΠΎΠ΄. ΠΠ΅Π½ΠΎΡΠΈΠΏΠΈΡΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΡΒ ΠΏΡΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ΠΌ Π°Π»Π»Π΅Π»Ρ-ΡΠΏΠ΅ΡΠΈΡΠΈΡΠ½ΠΎΠΉ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΈΠΈ ΡΒ Π΄Π΅ΡΠ΅ΠΊΡΠΈΠ΅ΠΉ ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΠΎΠ² Π²Β ΡΠ΅ΠΆΠΈΠΌΠ΅ ΡΠ΅Π°Π»ΡΠ½ΠΎΠ³ΠΎ Π²ΡΠ΅ΠΌΠ΅Π½ΠΈ ΡΒ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΠ΅ΠΌ TaqMan-Π·ΠΎΠ½Π΄ΠΎΠ², ΠΊΠΎΠΌΠΏΠ»Π΅ΠΌΠ΅Π½ΡΠ°ΡΠ½ΡΡ
ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠ½ΡΠΌ ΡΡΠ°ΡΡΠΊΠ°ΠΌ ΠΠΠ, ΠΈΒ Π°ΠΌΠΏΠ»ΠΈΡΠΈΠΊΠ°ΡΠΎΡΠ° CFX96 Real Time System (βBio-Radβ, Π‘Π¨Π). ΠΠ·ΡΡΠ°Π»ΠΈ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΡ rs9939609 Π³Π΅Π½Π° ΡΠ²ΡΠ·ΠΈ ΡΒ ΠΆΠΈΡΠΎΠ²ΠΎΠΉ ΠΌΠ°ΡΡΠΎΠΉ ΠΈΒ ΠΎΠΆΠΈΡΠ΅Π½ΠΈΠ΅ΠΌ (FTO), ΠΌΠ΅ΡΡΠΎΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΠ΅ 16q12.2, Π°Β ΡΠ°ΠΊΠΆΠ΅ rs2228570 Π³Π΅Π½Π° ΡΠ΅ΡΠ΅ΠΏΡΠΎΡΠ° Π²ΠΈΡΠ°ΠΌΠΈΠ½Π° D (VDR), ΠΌΠ΅ΡΡΠΎΠΏΠΎΠ»ΠΎΠΆΠ΅Π½ΠΈΠ΅ 12q13.11.Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΡΡΠ°ΠΆΠ΅Π½Π½ΡΠΉ Π΄Π΅ΡΠΈΡΠΈΡ Π²ΠΈΡΠ°ΠΌΠΈΠ½Π°Β D (ΡΡΠΎΠ²Π΅Π½Ρ 25(ΠΠ)D Π²Β ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ 20 Π½Π³/ΠΌΠ») ΠΎΡΠΌΠ΅ΡΠ°Π»ΡΡ ΡΒ 39,7% (54/136) Π½Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΡΡΠ΅Π΄Π½Π΅ΠΉ ΠΏΠΎΠ»ΠΎΡΡ Π ΠΎΡΡΠΈΠΈ, ΡΒ 40% (14/35) ΠΏΡΠΈΡΠ»ΡΡ
ΠΈΒ ΡΒ 30,7% (43/140) ΠΊΠΎΡΠ΅Π½Π½ΡΡ
ΠΆΠΈΡΠ΅Π»Π΅ΠΉ ΠΡΠ°ΠΉΠ½Π΅Π³ΠΎ Π‘Π΅Π²Π΅ΡΠ° (Π½Π΅Π½ΡΡ). ΠΡΠΈ ΠΎΠΆΠΈΡΠ΅Π½ΠΈΠΈ ΡΒ ΠΆΠΈΡΠ΅Π»Π΅ΠΉ ΡΡΠ΅Π΄Π½Π΅ΠΉ ΠΏΠΎΠ»ΠΎΡΡ Π ΠΎΡΡΠΈΠΈ ΡΡΠΎΠ²Π΅Π½Ρ 25(ΠΠ)D Π²Β ΡΡΠ²ΠΎΡΠΎΡΠΊΠ΅ ΠΊΡΠΎΠ²ΠΈ Π±ΡΠ» Π½ΠΈΠΆΠ΅, Π°Β ΡΒ ΠΊΠΎΡΠ΅Π½Π½ΠΎΠ³ΠΎ Π½Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΠΡΠ°ΠΉΠ½Π΅Π³ΠΎ Π‘Π΅Π²Π΅ΡΠ°Β β Π²ΡΡΠ΅, ΡΠ΅ΠΌ ΠΏΡΠΈ ΠΠΠ’30 (Ρ0,05). Π£Β ΠΊΠΎΡΠ΅Π½Π½ΠΎΠ³ΠΎ Π½Π°ΡΠ΅Π»Π΅Π½ΠΈΡ ΡΠ΅Π²Π΅ΡΠ½ΠΎΠ³ΠΎ ΡΠ΅Π³ΠΈΠΎΠ½Π° Π²ΡΡΠ²Π»Π΅Π½Π° ΡΡΠ°ΡΠΈΡΡΠΈΡΠ΅ΡΠΊΠΈ Π·Π½Π°ΡΠΈΠΌΠ°Ρ ΡΠ²ΡΠ·Ρ Π°Π»Π»Π΅Π»Ρ Π‘Β ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΠ° rs2228570 Π³Π΅Π½Π° VDR (ΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠ΅ ΡΠ°Π½ΡΠΎΠ² (ΠΠ¨) 2,5, 95% Π΄ΠΎΠ²Π΅ΡΠΈΡΠ΅Π»ΡΠ½ΡΠΉ ΠΈΠ½ΡΠ΅ΡΠ²Π°Π» (ΠΠ) [1,46β4,27], p=0,0006) ΠΈΒ Π³Π΅Π½ΠΎΡΠΈΠΏΠ° ΠΠ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΠ° rs9939609 Π³Π΅Π½Π° FTO (ΠΠ¨ 8,83, 95% ΠΠ [0,94β82,5], p=0,02) ΡΒ Π΄Π΅ΡΠΈΡΠΈΡΠΎΠΌ Π²ΠΈΡΠ°ΠΌΠΈΠ½Π° D.ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΡΡΠΎΡΠΈΠ°ΡΠΈΡ ΠΌΠ΅ΠΆΠ΄Ρ ΠΎΠΆΠΈΡΠ΅Π½ΠΈΠ΅ΠΌ ΠΈΒ ΠΎΠ±Π΅ΡΠΏΠ΅ΡΠ΅Π½Π½ΠΎΡΡΡΡ Π²ΠΈΡΠ°ΠΌΠΈΠ½ΠΎΠΌΒ D ΡΒ Π»ΡΠ΄Π΅ΠΉ ΡΒ ΠΏΠΎΠ»ΠΈΠΌΠΎΡΡΠΈΠ·ΠΌΠ°ΠΌΠΈ rs2228570 Π³Π΅Π½Π° VDR ΠΈΒ rs9939609 Π³Π΅Π½Π° FTO Π·Π°Π²ΠΈΡΠΈΡ ΠΎΡ ΠΈΡ
ΡΡΠ½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΏΡΠΈΠ½Π°Π΄Π»Π΅ΠΆΠ½ΠΎΡΡΠΈ.