1 research outputs found
Role Of Peroxisome Proliferator- Activated Receptor Gamma2 (Ppar-γG2) Gene Polymorphism In Type 2 Diabetes Mellitus
Objective: To find out the relation between Pro 12 Ala polymorphism
of peroxisome proliferator- activated receptor gamma2 (PPAR-g2) gene
with type 2 diabetes mellitus and the possible role of this gene
polymorphism as a link between obesity and type 2 diabetes mellitus.
Subjects and Methods: Subjects of this study were classified into 3
groups: (15) Apparently healthy lean individuals (Group I), (15) obese
non diabetic individuals (group II) and (24) patients with type 2
diabetes mellitus (group III). This group divided into: Diabetic non
obese patients (12 patients) (Group IIIa) and: Diabetic obese patients
(12 patients) (Group IIIb). The subjects were subjected to clinical
examination, serum insulin level and estimation of PPAR-g2 gene
polymorphism by Restriction fragment length polymorphism (RFLP)
polymerase chain reaction (PCR). Results: Frequency of Pro allele was
significant increase in diabetic non obese patients& diabetic non
obese patients when compared to control group (P= 0.048 and 0.003,
respectively). However, there was no difference between obese non
diabetic group and control group as regard the allelic frequencies (p=
0.462). While diabetic non obese patients had higher Pro allele
frequency (p= 0.003) than in diabetic obese patients (p= 0.048).
Insulin resistance by homeostasis model assessment (HOMA-IR) was
significantly increased in Pro/Pro carriers in group II and group III
when compared to that of Pro/Ala plus Ala/Ala carriers (p= 0.004 and
0.023; respectively). Conclusion: Pro12 allele of PPAR-g2 gene could
be a genetic risk factor for insulin resistance and type 2 diabetes
mellitus