Fundamental Studies on Measurement of Plasma lα,25-Dihydroxycholecalciferol Concentration by Radioreceptor Assay

It is well known that 1α,25-dihydroxyvitamin D(lα,25 (OH)2D) plays an important role in the pathophysiology of bone and calcium metabolism. In present paper, we established a relatively convenient assay system, using radioreceptor assay, for lα,25 (OH) 2D. Fundamental studies showed that this assay system had good sensitivity enough to detect 2 pg of lα,25 (OH) 2D per tube, and had good reproducibility. Therefore, it was shown that this assay system could be applied for clinical use. The plasma concentrations of lα,25(OH)2D, measured by this assay system, in healthy young males and females, and in aged females were 55.7±16.1, 44.8±22.1 and 23.5±13.0 pg/ml (mean±s.d.), respectively. Thus, the aged females showed significantly lower (p<0.005) lα,25 (OH)2D levels than the young group. This fact suggests that vitamin D deficient state might exist in aged females

Morphologic change and the DNA contents of the testicular cell of rabbits treated with the fatty acid ex­tracted from the irradiated animals

1. The unsaturated fatty acid fraction (OX) from the liver of irradiated rabbits contains substance which has the same effects as X-ray irradiation on the testicular cells. 2. This substance introduced intravenously causes the degeneration of the germinal cells with the formation of giant cells or multi-nucleated cells and the mitotic abnormalities. 3. The DNA content of the cell also shows the changes exactly identical with that seen after X-ray irradiation. 4. From these results we conclude that the X-ray injury will be mainly due to the production of some toxic substance which is found in the unsaturated fatty acid fraction and severely affects the cells in mitosis and DNA metabolism.</p

Effects of Treatments for Experimental Bone Tumor on Prostaglandin E Level and Bone Scintigrams

The role of Prostaglandin E (PgE) level was studied experimentally as follows: 1) intrahepatic implantation of VX-2, 2) intravenous injection of VX-2, 3) effect of treatments on intramedullary implanted VX-2. The levels of PgE in intrahepatic and intravenous transplantation were not higher than that of intramedullary transplantation. Mitomycin C (MMC) did not reduce the PgE level and appearance time of bone scan abnormality was the same as that of untreated animals. A combination of indomethacin and MMC caused a delay in appearance time of bone scan abnormalities

Morphologic studies of bone marrow cells exposed to the phospholipid fraction from the liver of irradiated animal, an experiment in vitro

With the purpose of revealing the biological effects of the X-ray irradiation the authors extracted phospholipids from the liver of irradiated animals and proved that this substance has the action to inhibit the growth of the bone marrow cells, the motility of pseudo-eosinophilis and the erythropoiesis in tissue culture, suggesting that the injury will mainly be induced by the toxic substances produced by irradiation.</p

Assessment of Bone Scintigraphy Showing Markedly Increased Accumulation in Mandibular and Maxillary Lesions of Cementifying Fibroma

This report concerns with three cases of cementifying fibroma showing markedly increased accumulation of 99mTc-MDP in mandibular and maxillary lesions. The bone scintigraphic and histopathological findings are presented and discussed

Scintigraphic Findings of Bone and Bone-Marrow and Determination of Bone Mineral Density Using Photon Absorptiometry in Osteopetrosis

On a 15-year-old girl with osteopetrosis, bone and bonemarrow scintigraphy were performed. Also, bone mineral density (BMD) with quantitative CT (QCT), single photon absorptiometry (SPA) and dual photon absorptiometry (DPA) were measured. On bone scintigraphy the diffusely increased skeletal uptake and relatively diminished renal uptake were noted. On the other hand, on bone marrow scintigraphy poor accumulation in central marrow and peripheral expansion were shown. BMD value by QCT and DPA (mainly trabecular bone) was markedly high, while BMD by SPA (mainly cortical bone) was within normal range. Thus, it was shown that bone and bone-marrow scintigraphy combined with BMD measurement by photon absorptiometry were useful and essential in evaluating the pathophysiology of osteosclerosis