In-vitro susceptibility of multiple drug resistant Pseudomonas aeruginosa to organic acids

Objectives: Pseudomonas aeruginosa is a classic opportunistic pathogen with innate resistance to many antibiotics anddisinfectants. Resistance to antimicrobial agents makes it the most noxious organism to eliminate from infection site. Inview of its antimicrobial resistance, an attempt was made to study its susceptibility to various organic acids.Methods: Seven clinical isolates of P. aeruginosa resistant to multiple antibiotics were subjected to in vitro susceptibilityto various organic acids by broth dilution method to find out susceptibility to various organic acids.Results: The isolates of P. aeruginosa resistant to 14 antimicrobials were found susceptible to one percent oxalic acidand trichloroacetic acid, two percent lactic acid and citric acid, and three percent acetic acid. It is interesting to note thatstrains resistant to multiple antibiotics were also found susceptible to organic acids. Oxalic acid and trichloroacetic acidwere found highly effective.Conclusions: Clinical use of oxalic acid, trichloroacetic acid and lactic acid as topical agents to treat superficial pseudomonalinfections caused by difficult strains of P. aeruginosa may be recommended after confirmation of their toxicityand in vivo efficacy in animal models. J Microbiol Infect Dis 2013; 3(2): 67-70Key words: Pseudomonas aeruginosa, Multiple Antibiotic Resistance, Susceptibility to Organic Acid

Effect Of Eccentric Muscle Training To Reduce Severity Of Delayed Onset Muscle Soreness In Athletic Subjects

Aim: To verify whether "Eccentric muscle training of quadriceps femoris muscle in athletes will be effective in minimizing the incidence and severity of delayed onset muscle soreness (DOMS)". Long distance runners suffer with delayed onset muscle soreness. Many interventions have been tried and reported. Methods : Forty long distance male runners between 18-25 years of age were included in this study. They were equally categorized in two groups. Control group consisted of the subjects who were given only stretching exercises. The experimental group was given eccentric repetitive low intensity eccentric muscle training of quadriceps femoris muscle along with stretching exercises. Visual Analog Scale (VAS) pain scale and Functional Independence Measure Score (FIMS) were used as prognostic tools. The data collected was analyzed with the help of unpaired "t" test and chi square test. Results : Experimental group showed improvement in pain scale compared to control group (t value= 2.02 at p< 0.05). FIMS showed significant improvement in experimental group (t value= 2.02 at p< 0.05). Conclusion: Eccentric training of quadriceps femoris muscle can be incorporated into warm up program for the long distance runners to reduce delayed onset muscle soreness

Stimulus-triggered Fate Conversion of Somatic Cells into Pluripotency in Chronic Wounds in Human Beings?

Bone-marrow derived stem cells are multi potential or totipotent and are able to differentiate into numerous cell types. Their application is indicated in various reconstructive and restorative surgeries for rapid healing. A technique for creating cells that have the embryonic ability to turn into almost any cell type in the mammalian body has been reported. Recently, an unexpected phenomenon of somatic cell reprogramming into pluripotent cells by exposing to sublethal stimuli such as citrate based acidic medium has been reported. With the concept of creating acidic environment in chronic infected wounds to make a condition unsuitable for growth and multiplication of bacteria using 3% citric acid has been reported. It would be interesting to study whether the phenomenon of pluripotency takes place in chronic infected wounds in human beings following the application of 3% citric acid and plays an important role in formation of healthy granulation tissue