Anti -Hcv Lectin from Egyptian Pisum sativum

Abstract: Lectins are carbohydrate binding proteins expressed in plants, animals and microorganisms and have been used to probe the surface properties of a wide range of prokaryotic and eukaryotic cells. The implication of some lectin molecules in several physiological processes has been claimed. The aim of this work is to purify the lectin from Egyptian pea (Pisum sativum) seeds and study its biochemical characterizations and potential as pharmaceutical compound. Egyptian Pea lectin seeds was purified by ion exchange chromatography (DEAE column) followed by Sephadex G 100 Column. Purified lectin showed Hemagglutinating activity with human and rabbit erythrocytes and appeared as one band of 32 kDa on SDS-PAGE gel, also amino acid analysis showed high content of glutamic and aspartic acids but very low levels of sulfur amino acids. The purified Egyptian pea (Pisum sativum) lectin showed high effect on HCV replication at concentrations of 20, 40 and 80 μg. Therefore, pea's lectins might consider as one of the natural compounds that can be used in designing a safe and effective anti HCV therapy

Phenolic content, antioxidant and antimicrobial activities of Egyptian date palm (Phoenix dactylifera L.) fruits

Date palm fruits are one of the most popular fruits packed with an impressive list of essential nutrients, vitamins, and minerals that are required for normal growth, development and overall well-being. They contain health benefiting polyphenolic antioxidants. This work is aimed to determine the phenolic profile and phenolic content of date palm fruits (Tamr stage) and evaluate their functional properties like antioxidant and antimicrobial activities to confirm the date palm health benefits. Water extract of Tamr stage showed a higher content (14.80 mg GAE/g sample) of phenolic compounds than ethanol extract (10.31mg GAE/g sample). HPLC analysis showed the extracts contain the high concentration in esculetin (15.11 and 17.30mg/100g) and tannic acid (2.85 and 1.79 mg/100g). On the other hand, protocatechuic acid, catechol, Pyrogallol and cinnamic acid didn’t detect in both extracts. Moderate concentrations of gallic acid (7.51 and 5.28 mg/100g), itaconic acid (6.40 and 5.91 mg/100g) and traces of ferulic acid (0.15 and 0.22 mg/100g) were detected. DPPH assay revealed a good antioxidant capacity of water extract, which was higher than of ethanol extract. Antimicrobial data exhibited an impressive antibacterial activity for date extract. Date extract showed a strong antibacterial activity (for water and ethanol extracts) against E. coli (20 ±0.57 and 16 ±0.57 mm), Salmonella enterica (20 ±0.54 and 14 ±0.52 mm) and Bacillus sabtilis (18±0.32, 15±0.23 mm) and moderate inhibition against staphylococcus areues (8 ±0.48 and 5 ±0.52 mm) and Enterococcus faecalis (5 ±0.36 and 2 ±0.57mm). These findings may enhance our knowledge for the value and importance of using the dates in our daily diet; and can be used as natural antioxidants and antimicrobial agents for various food products

Phenolic Content, Antioxidant and Antimicrobial activities of Egyptian Date Palm (Phoenix dactylifera L.) Fruits

Date palm fruits are one of the most popular fruits packed with an impressive list of essential nutrients, vitamins, and minerals that are required for normal growth, development and overall well-being. They contain health benefiting polyphenolic antioxidants. This work is aimed to determine the phenolic profile and phenolic content of date palm fruits (Tamr stage) and evaluate their functional properties like antioxidant and antimicrobial activities to confirm the date palm health benefits. Water extract of Tamr stage showed a higher content (14.80 mg GAE/g sample) of phenolic compounds than ethanol extract (10.31mg GAE/g sample). HPLC analysis showed the extracts contain the high concentration in esculetin (15.11 and 17.30mg/100g) and tannic acid (2.85 and 1.79 mg/100g). On the other hand, protocatechuic acid, catechol, Pyrogallol and cinnamic acid didn't detect in both extracts. Moderate concentrations of gallic acid (7.51 and 5.28 mg/100g), itaconic acid (6.40 and 5.91 mg/100g) and traces of ferulic acid (0.15 and 0.22 mg/100g) were detected. DPPH assay revealed a good antioxidant capacity of water extract, which was higher than of ethanol extract. Antimicrobial data exhibited an impressive antibacterial activity for date extract. Date extract showed a strong antibacterial activity (for water and ethanol extracts) against E. coli (20 ±0.57 and 16 ±0.57 mm), Salmonella enterica (20 ±0.54 and 14 ±0.52 mm) and Bacillus sabtilis (18±0.32, 15±0.23 mm) and moderate inhibition against staphylococcus areues (8 ±0.48 and 5 ±0.52 mm) and Enterococcus faecalis (5 ±0.36 and 2 ±0.57mm). These findings may enhance our knowledge for the value and importance of using the dates in our daily diet; and can be used as natural antioxidants and antimicrobial agents for various food product

ACCOMPANIMENT OF THE POLYMORPHISM AT DEFENSIN GENE LOCI WITH MILK PRODUCTIVITY IN HOLSTEIN FRIESIAN AND EGYPTIAN COWS **

**Original scientific paper- Originalni naučni rad Abstract: Because of the antimicrobial role that defensins play in cows, genes encoding these peptides may be considered as molecular markers of a genetically determined susceptibility of the mammary gland to mastitis. In Friesian and Egyptian cows which were selected based on their milk productivity, 1638-bp beta-defensin genes were amplified. Two PCR amplicon sizes of the gene encoding beta-defensin (1638 bp and 429 bp) were observed in Friesian cows (high milk production), while in Egyptian cows (low milk production) one PCR amplicon size (429 bp) was observed. PCR–RFLP technique was used to discriminate between the common 429 bp band in both Friesian and Egyptian cows, but no difference between them had been observed. DNA sequencing for 1638 bp (B2) and 429 bp (B1) was carried out. Sequence analysis indicated that these two PCR amplicon sizes were two types of genes encoding beta-defensins and very tightly close to each other. Based on their sequence alignment (B1 and B2) with the presented defensin genes in the GenBank, phylogenic tree was constructed. A new gene (B1) belongs to the beta-defensin genes family was detected for the first time and associated to the low milk production in cattle population