The potential effects and interactions of oxidative stress and trace minerals on fresh and frozen semen in bulls - a review

Reproduction is one of the most important factors determining successful cattle farming systems. Management practices, such as nutritional supplementation, can influence the reproductive performance of cattle. The objective of this literature review is to determine the potential value of injectable trace mineral administration on fresh and cryopreserved semen quality of bulls. A search of keywords related to the topic was performed on published articles and textbooks. The search was narrowed to the 40 most relevant references. Several studies have demonstrated a positive association between trace mineral supplementation and bull semen quality. Moderate amounts of reactive oxygen species (ROS) play an important role in normal spermatogenesis, but oxidative stress (OS), as experienced with adverse environmental conditions or disease, can contribute to idiopathic male infertility by negatively impacting spermatogenesis. Trace minerals such as selenium, copper, zinc, and manganese have been demonstrated to have antioxidant effects in mammals. Due to the complexity of oral ingested trace mineral bioavailability, injectable trace mineral supplementation prior to physiological periods with known deficiencies or increased requirement can benefit the animal. The potential benefits of injectable trace mineral supplementation to minimise oxidative damage to spermatogenesis in breeding bulls need further investigation. Positive results from such studies can lead to the implementation of injectable trace mineral supplementation strategies prior to the breeding season to minimise the detrimental effects of OS and can improve semen quality.http://www.jsava.co.zaam2023Production Animal Studie

Serum albumin concentration of donor cows as an indicator of developmental competence of oocytes

Adequate nutrition is required for maintenance of normal reproduction in cattle. Albumin, the best marker and fundamental part of nutrition, most abundant plasma protein and major component of fetal bovine serum, is the best predictor of malnourishment in South African cattle. The aim of this study was to determine if serum albumin concentrations of donor cows predict the developmental competence of oocytes, and if additional protein supplementation of the in vitro culture media improves embryo outcomes in oocytes from cows with inadequate serum albumin concentrations. Oocytes (n = 1024) were recovered from donors with inadequate (≤35.9 g/L), or adequate serum albumin concentrations (≥36.0 g/L). Four hundred and sixty oocytes originated from cows with inadequate serum albumin and 564 from cows with adequate serum albumin. Oocytes of these cohorts were randomly allocated to a control and supplemented fetal bovine serum in vitro embryo culture protocol. Multiple linear, logistic and Poisson regression analyses were performed to estimate the effects of different covariates on linear, binary and count data respectively. Mixed effects Poisson regression was performed for the number of oocytes that developed into blastocysts by the seventh day of culture. Adequate serum albumin concentration of donor cows independently resulted in 46% increased blastocyst formation in the control protocol (P = 0.02). Although fetal bovine serum supplementation of the culture protocol did not affect blastocyst formation in oocytes originating from cows with inadequate serum albumin, it independently reduced blastocyst formation by 30% in oocytes originating from cows with adequate serum albumin (P = 0.02). Other independent predictors of blastocyst outcome included higher serum urea nitrogen, lower beta (β)-hydroxybutyric acid concentrations and lower fat classification of donor cows. It is concluded that adequate serum albumin of donor cows is a significant predictor of developmental competence of oocytes, and that in vitro supplementation of fetal bovine serum does not improve developmental competence of oocytes and can lead to negative blastocyst outcomes. Further research is required to determine optimal protein supplementation for oocytes originating from inadequately nourished cows.The Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria.http://www.theriojournal.com2020-02-01hj2018Production Animal Studie