The Primordial Abundance of He4: An Update

We include new data in an updated analysis of helium in low metallicity extragalactic HII regions with the goal of deriving the primordial abundance of He4 (Y_P). We show that the new observations of Izotov et al (ITL) are consistent with previous data. However they should not be taken in isolation to determine (Y_P) due to the lack of sufficiently low metallicity points. We use the extant data in a semi-empirical approach to bounding the size of possible systematic uncertainties in the determination of (Y_P). Our best estimate for the primordial abundance of He4 assuming a linear relation between He4 and O/H is Y_P = 0.230 \pm 0.003 (stat) based on the subset of HII regions with the lowest metallicity; for our full data set we find Y_P = 0.234 \pm 0.002 (stat). Both values are entirely consistent with our previous results. We discuss the implications of these values for standard big bang nucleosynthesis (SBBN), particularly in the context of recent measurements of deuterium in high redshift, low metallicity QSO absorption-line systems.Comment: 26 pages, latex, 6 ps figure

The He abundance in the metal-deficient blue compact dwarf galaxies Tol 1214-277 and Tol 65

We present high-quality Keck telescope spectroscopic observations of the two metal-deficient blue compact dwarf (BCD) galaxies Tol 1214-277 and Tol 65. These data are used to derive the heavy-element and helium abundances. We find that the oxygen abundances in Tol 1214-277 and Tol 65 are the same, 12+logO/H=7.54+/-0.01, or Zsun/24, despite the different ionization conditions in these galaxies. The nitrogen-to-oxygen abundance ratio in both galaxies is logN/O=-1.64+/-0.02 and lies in the narrow range found for the other most metal-deficient BCDs. We use the five strongest HeI emission lines 3889, 4471, 5876, 6678 and 7065, to correct self-consistently their intensities for collisional and fluorescent enhancement mechanisms and to derive the He abundance. Underlying stellar absorption is found to be important for the HeI 4471 emission line in both galaxies, being larger in Tol 65. The weighted He mass fractions in Tol 1214-277 and Tol 65 are respectively Y=0.2458+/-0.0039 and 0.2410+/-0.0050 when the three HeI emission lines, 4471, 5876 and 6678, are used, and are, respectively, 0.2466+/-0.0043 and 0.2463+/-0.0057 when the HeI 4471 emission line is excluded. These values are in very good agreement with recent measurements of the He mass fraction in others of the most metal-deficient BCDs by Izotov and coworkers. We find that the combined effect of the systematic uncertainties due to the underlying HeI stellar absorption lines, ionization and temperature structure of the HII region and collisional excitation of the hydrogen emission lines is likely small, not exceeding ~2% (the error is 2sigma). Our results support the validity of the standard big bang model of nucleosynthesis.Comment: 22 pages, 3 Postscript figures, accepted for publication in the Astrophysical Journa

Improving Predictions for Helium Emission Lines

We have combined the detailed He I recombination model of Smits with the collisional transitions of Sawey & Berrington in order to produce new accurate helium emissivities that include the effects of collisional excitation from both the 2 (3)S and 2 (1) S levels. We present a grid of emissivities for a range of temperature and densities along with analytical fits and error estimates. Fits accurate to within 1% are given for the emissivities of the brightest lines over a restricted range for estimates of primordial helium abundance. We characterize the analysis uncertainties associated with uncertainties in temperature, density, fitting functions, and input atomic data. We estimate that atomic data uncertainties alone may limit abundance estimates to an accuracy of 1.5%; systematic errors may be greater than this. This analysis uncertainty must be incorporated when attempting to make high accuracy estimates of the helium abundance. For example, in recent determinations of the primordial helium abundance, uncertainties in the input atomic data have been neglected.Comment: ApJ, accepte

The effect of collisional enhancement of Balmer lines on the determination of the primordial helium abundance

This paper describes a new determination of the primordial helium abundance (Y_P), based on the abundance analysis of five metal-poor extragalactic HII regions. For three regions of the sample (SBS 0335-052, I Zw 18, and H29) we present tailored photoionization models based on improved calculations with respect to previous models. In particular, we use the photoionization models to study quantitatively the effect of collisional excitation of Balmer lines on the determination of the helium abundance (Y) in the individual regions. This effect is twofold: first, the intensities of the Balmer lines are enhanced with respect to the pure recombination value, mimicking a higher hydrogen abundance; second, the observed reddening is larger than the true extinction, due to the differential effect of collisions on different Balmer lines. In addition to these effects, our analysis takes into account the following features of HII regions: (i) the temperature structure, (ii) the density structure, (iii) the presence of neutral helium, (iv) the collisional excitation of the HeI lines, (v) the underlying absorption of the HeI lines, and (vi) the optical thickness of the HeI lines. The object that shows the highest increase in Y after the inclusion of collisional effects in the analysis is SBS 0335-052, whose helium abundance has been revised by Delta Y = +0.0107. The revised Y values for the five objects in our sample yield an increase of +0.0035 in Y_P, giving Y_P = 0.2391 +/- 0.0020.Comment: 59 pages, 8 figures. AAS Latex. Accepted for publication in the Astrophysical Journa

Systematic effects and a new determination of the primordial abundance of 4He and dY/dZ from observations of blue compact galaxies

We use spectroscopic observations of a sample of 82 HII regions in 76 blue compact galaxies to determine the primordial helium abundance Yp and the slope dY/dZ from the Y-O/H linear regression. To improve the accuracy of the dY/dZ measurement, we have included new spectrophotometric observations of 33 HII regions which span a large metallicity range, with oxygen abundance 12+log(O/H) varying between 7.43 and 8.30 (Zsun/30<Z<Zsun/4). For a subsample of 7 HII regions, we derive the He mass fraction taking into account known systematic effects, including collisional and fluorescent enhancements of HeI emission lines, collisional excitation of hydrogen emission, underlying stellar HeI absorption and the difference between the temperatures Te(HeII) in the He^+ zone and Te(OIII) derived from the collisionally excited [OIII] lines. We find that the net result of all the systematic effects combined is small, changing the He mass fraction by less than 0.6%. By extrapolating the Y vs. O/H linear regression to O/H=0 for 7 HII regions of this subsample, we obtain Yp=0.2421+/-0.0021 and dY/dO=5.7+/-1.8, which corresponds to dY/dZ=3.7+/-1.2, assuming the oxygen mass fraction to be O=0.66Z. In the framework of the standard Big Bang nucleosynthesis theory, this Yp corresponds to Omega_b h^2 = 0.012^+0.003_-0.002, where h is the Hubble constant in units of 100 km/s/Mpc. This is smaller at the 2sigma level than the value obtained from recent deuterium abundance and microwave background radiation measurements. The linear regression slope dY/dO=4.3+/-0.7 (corresponding to dY/dZ=2.8+/-0.5) for the whole sample of 82 HII regions is similar to that derived for the subsample of 7 HII regions, although it has a considerably smaller uncertainty.Comment: 53 pages, 3 Postscript figures, accepted for publication in the Astrophysical Journa

Using Intratumor Heterogeneity of Immunohistochemistry Biomarkers to Classify Laryngeal and Hypopharyngeal Tumors Based on Histologic Features

Haralick texture features are used to quantify the spatial distribution of signal intensities within an image. In this study, the heterogeneity of proliferation (Ki-67 expression) and immune cells (CD45 expression) within tumors was quantified and used to classify histologic characteristics of larynx and hypopharynx carcinomas. Of 21 laryngectomy specimens, 74 whole-mount tumor slides were scored on histologic characteristics. Ki-67 and CD45 immunohistochemistry was performed, and all sections were digitized. The tumor area was annotated in QuPath. Haralick features independent of the diaminobenzidine intensity were extracted from the isolated diaminobenzidine signal to quantify intratumor heterogeneity. Haralick features from both Ki-67 and CD45 were used as input for a principal component analysis. A linear support vector machine was fitted to the first 4 principal components for classification and validated with a leave-one-patient-out cross-validation method. Significant differences in individual Haralick features were found between cohesive and noncohesive tumors for CD45 (angular second motion: P =.03, inverse difference moment: P =.009, and entropy: P =.02) and between the larynx and hypopharynx tumors for both CD45 (angular second motion: P =.03, inverse difference moment: P =.007, and entropy: P =.005) and Ki-67 (correlation: P =.003). Therefore, these features were used for classification. The linear classifier resulted in a classification accuracy of 85% for site of origin and 81% for growth pattern. A leave-one-patient-out cross-validation resulted in an error rate of 0.27 and 0.35 for both classifiers, respectively. In conclusion, we show a method to quantify intratumor heterogeneity of immunohistochemistry biomarkers using Haralick features. This study also shows the feasibility of using these features to classify tumors by histologic characteristics. The classifiers created in this study are a proof of concept because more data are needed to create robust classifiers, but the method shows potential for automated tumor classification.</p

An imaging and spectroscopic study of the very metal-deficient blue compact dwarf galaxy Tol 1214--277

We present a spectrophotometric study based on VLT/FORS I observations of one of the most metal-deficient blue compact dwarf (BCD) galaxies known, Tol 1214-277 (Z ~ Zsun/25). The data show that roughly half of the total luminosity of the BCD originates from a bright and compact starburst region located at the northeastern tip of a faint dwarf galaxy with cometary appearance. The starburst has ignited less than 4 Myr ago and its emission is powered by several thousands O7V stars and ~ 170 late-type nitrogen Wolf-Rayet stars located within a compact region with < 500 pc in diameter. For the first time in a BCD, a relatively strong [Fe V] 4227 emission line is seen which together with intense He II 4686 emission indicates the presence of a very hard radiation field in Tol 1214-277. We argue that this extraordinarily hard radiation originates from both Wolf--Rayet stars and radiative shocks in the starburst region. The structural properties of the low-surface-brightness (LSB) component underlying the starburst have been investigated by means of surface photometry down to 28 B mag/sq.arcsec. We find that, for a surface brightness level fainter than ~ 24.5 B mag/sq.arcsec, an exponential fitting law provides an adequate approximation to its radial intensity distribution. The broad-band colors in the outskirts of the LSB component of Tol 1214-277 are nearly constant and are consistent with an age below one Gyr. This conclusion is supported by the comparison of the observed spectral energy distribution (SED) of the LSB host with theoretical SEDs.Comment: 17 pages, 11 Postscript figures, uses emulateapj.sty, to appear in Astronomical Journa