Determining Atg11\u27s binding interactions using a yeast two hybrid system and directed mutagenesis

Autophagy is a mechanism of intracellular degradation within eukaryotes. Because of its aid to a cells longevity, autophagy is believed to be important for the prevention of neurodegenerative diseases. Autophagy related protein 11 (Atg11) is a coiled-coil scaffolding protein required for selective autophagy in yeast. Atg11 is known to interact with Atg1, Atg20, and Atg29, in addition to connecting cargo molecules prApel -Atg19 to the phagophore assembly site. In addition to its many functions within yeast autophagy, Atg11 has two human homologs: Huntingtin protein and FIP200. This homology encourages us to gain a further understanding of Atg11\u27s binding sites. In this study we show that making two mutations within the coiled-coil domain 2 of Atg11, 1562E/Y565E, causes it to lose its ability to interact with Atg1

Woolworths and Wales: A Multi-Dimensional Analysis of the Loss of a Local Brand

In this paper we present a multi-dimensional analysis of the closure of Woolworths in Wales and the way in which the loss of this familiar high-street brand can be accounted for at a number of levels and within different social arenas. Primarily, the paper demonstrates how Woolworths is positioned as a symbol a previous era of consumption centred upon community and place based notions of nostalgia and community. What is striking in the analysis is the similarities in the way in which Woolworths is mobilised as a symbol by the general public and elites; albeit with varying outcomes and affects. In presenting the analysis the paper demonstrates a processual framing as providing a fruitful approach to the combination of different approaches and fields of inquiry (sociology, geography, and political science) without diminishing their distinct contributions.Recession, 'Credit Crunch', Community, Economy, the High-Street, Welsh Assembly Government, Woolworths, Consumption

A Phosphate Analysis of Stone-Walled Structures in the Suikerbosrand Nature Reserve

Research Report submitted in partial fulfilment of the requirements of the Degree of BSc Honours in ArchaeologyWith the purpose of confirming the “kraal index” created by Sadr and Rodier (2012), a group of stone-walled structures in the Suikerbosrand Nature Reserve were selected for study. Confirming this involved testing for livestock presence in the inner enclosures of Group III stone-walled complexes. Phosphate testing of the inner enclosures revealed the absence of evidence showing the presence of livestock occupation. This may be because of the phosphate testing method used. It may also be because of different culture factors involving the recycling of dung as fuel and kraal maintenance. The probability of each hypothesis is weighed up against the supporting data captured by the phosphate analyses