COMPUTER-DRIVEN BIOTECHNICAL SYSTEMS FOR THE STUDY AND SELF-DEVELOPMENT OF MOTION ACTIVITY OF CHILDREN AGED 4-7

INTRODUCTION: Due to the increasing involvement of children in sports at earlier ages, it is a necessary to scientifically study children’s motion activity and to assist in its coordinated aimed development. The failure of children to perceive and formulate aims and motion tasks contributes to the complexity of the problem [1,2]. It is proposed that the application of the suggested method of “playing-mode reflection” will speed up the mental and motion self-development of children. METHOD: A set of computer-driven sports training equipment is created, allowing us to investigate the physical, mental and intellectual abilities of trainees in the playing mode and differentially assign parameters of the above mentioned characteristics to the system, appropriate to children’s ages, conditions and development levels. Bio-technical training systems possess measurement, computational and control functions and include: IBM-compatible multimedia computer; five specially designed loading devices (training set) with sensors and I/O data exchange units; unique computer software programs for testing and teaching children during play; methods of bio-reflective control of children’s behavior, allowing us to plan, individualize and differentiate the training load in magnitude and duration depending on the trainee’s situation. Sets of bio-technical training equipment were installed in 50 pre-school institutions in Russia. Systematic tests of the motion activities of children were continuously performed on 210 children from 4 to 7 years of age using the following parameters: frequencies of arm and leg motions during pedaling; speed of rotation motion in joints; reaction time to signals by arms and the whole body; degree of stability and posture correction frequency during balance retention on a movable support. RESULTS AND DISCUSSION: An Educational program for children’s selfdevelopment using bio-technical training systems has been successfully created. The system contributes to correct carriage, actions and motion activities on the part of children. It has been demonstrated that children’s development according to the suggested program with the help of bio-technical systems provides more effective results In comparison with the case where no bio-technical systems are used. REFERENCES: [1]. L.I. Lubysheva. (1996). Theory and Practice of Physical culture, 1. [2]. D.D. Donskoy, S.V. Dmitriev.(1997). Integration Problems of Natural and Humanitarian Sciences. (pp.58-63).Nishny Novgorod, Russia

Itt1p, a novel protein inhibiting translation termination in Saccharomyces cerevisiae

BACKGROUND: Termination of translation in eukaryotes is controlled by two interacting polypeptide chain release factors, eRFl and eRF3. eRFl recognizes nonsense codons UAA, UAG and UGA, while eRF3 stimulates polypeptide release from the ribosome in a GTP- and eRFl – dependent manner. Recent studies has shown that proteins interacting with these release factors can modulate the efficiency of nonsense codon readthrough. RESULTS: We have isolated a nonessential yeast gene, which causes suppression of nonsense mutations, being in a multicopy state. This gene encodes a protein designated Itt1p, possessing a zinc finger domain characteristic of the TRIAD proteins of higher eukaryotes. Overexpression of Itt1p decreases the efficiency of translation termination, resulting in the readthrough of all three types of nonsense codons. Itt1p interacts in vitro with both eRFl and eRF3. Overexpression of eRFl, but not of eRF3, abolishes the nonsense suppressor effect of overexpressed Itt1p. CONCLUSIONS: The data obtained demonstrate that Itt1p can modulate the efficiency of translation termination in yeast. This protein possesses a zinc finger domain characteristic of the TRIAD proteins of higher eukaryotes, and this is a first observation of such protein being involved in translation

DBSolve Optimum: a software package for kinetic modeling which allows dynamic visualization of simulation results

<p>Abstract</p> <p>Background</p> <p>Systems biology research and applications require creation, validation, extensive usage of mathematical models and visualization of simulation results by end-users. Our goal is to develop novel method for visualization of simulation results and implement it in simulation software package equipped with the sophisticated mathematical and computational techniques for model development, verification and parameter fitting.</p> <p>Results</p> <p>We present mathematical simulation workbench DBSolve Optimum which is significantly improved and extended successor of well known simulation software DBSolve5. Concept of "dynamic visualization" of simulation results has been developed and implemented in DBSolve Optimum. In framework of the concept graphical objects representing metabolite concentrations and reactions change their volume and shape in accordance to simulation results. This technique is applied to visualize both kinetic response of the model and dependence of its steady state on parameter. The use of the dynamic visualization is illustrated with kinetic model of the Krebs cycle.</p> <p>Conclusion</p> <p>DBSolve Optimum is a user friendly simulation software package that enables to simplify the construction, verification, analysis and visualization of kinetic models. Dynamic visualization tool implemented in the software allows user to animate simulation results and, thereby, present them in more comprehensible mode. DBSolve Optimum and built-in dynamic visualization module is free for both academic and commercial use. It can be downloaded directly from <url>http://www.insysbio.ru</url>.</p