A scheme with two large extra dimensions confronted with neutrino physics

We investigate a particle physics model in a six-dimensional spacetime, where two extra dimensions form a torus. Particles with Standard Model charges are confined by interactions with a scalar field to four four-dimensional branes, two vortices accommodating ordinary type fermions and two antivortices accommodating mirror fermions. We investigate the phenomenological implications of this multibrane structure by confronting the model with neutrino physics data.Comment: LATEX, 24 pages, 9 figures, minor changes in the tex

Resistance of Gram-positive bacteria to nisin is not determined by Lipid II levels

Lipid II is essential for nisin-mediated pore formation at nano-molar concentrations. We tested whether nisin resistance could result from different Lipid II levels, by comparing the maximal Lipid II pool in Micrococcus flavus (sensitive) and Listeria monocytogenes (relatively insensitive) and their nisin-resistant variants, with a newly developed method. No correlation was observed between the maximal Lipid II pool and nisin sensitivity, as was further corroborated by using spheroplasts of nisin-resistant and wild-type strains of M. flavus, which were equally sensitive to nisin. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved

Comparison of quenching and extraction methodologies for metabolome analysis of Lactobacillus plantarum

<p>Abstract</p> <p>Background</p> <p>A reliable quenching and metabolite extraction method has been developed for <it>Lactobacillus plantarum</it>. The energy charge value was used as a critical indicator for fixation of metabolism.</p> <p>Results</p> <p>Four different aqueous quenching solutions, all containing 60% of methanol, were compared for their efficiency. Only the solutions containing either 70 mM HEPES or 0.85% (w/v) ammonium carbonate (pH 5.5) caused less than 10% cell leakage and the energy charge of the quenched cells was high, indicating rapid inactivation of the metabolism.</p> <p>The efficiency of extraction of intracellular metabolites from cell cultures depends on the extraction methods, and is expected to vary between micro-organisms. For <it>L. plantarum</it>, we have compared five different extraction methodologies based on (i) cold methanol, (ii) perchloric acid, (iii) boiling ethanol, (iv) chloroform/methanol (1:1) and (v) chloroform/water (1:1). Quantification of representative intracellular metabolites showed that the best extraction efficiencies were achieved with cold methanol, boiling ethanol and perchloric acid.</p> <p>Conclusion</p> <p>The ammonium carbonate solution was selected as the most suitable quenching buffer for metabolomics studies in <it>L. plantarum </it>because (i) leakage is minimal, (ii) the energy charge indicates good fixation of metabolism, and (iii) all components are easily removed during freeze-drying. A modified procedure based on cold methanol extraction combined good extractability with mild extraction conditions and high enzymatic inactivation. These features make the combination of these quenching and extraction protocols very suitable for metabolomics studies with <it>L. plantarum</it>.</p

Treatment responsiveness of replicated psychopathy profiles.

Theory and accumulating data suggest systematic heterogeneity among offenders with psychopathic traits. Several empirical investigations converge on the nature of subtypes, but little is known about differences in treatment responsivity. We have used the 4-facet model of the Psychopathy Checklist-Revised (PCL-R) to provide a framework for detecting subtypes. The present study used the full range of PCL-R scores in a sample of male violent offenders (N = 190) to replicate subtypes found in a partly overlapping sample by Neumann, Vitacco, and Mokros (2016), using Latent Profile Analysis (LPA), and subsequently to examine potential differences in treatment responsivity. Four subtypes emerged. Within the prototypical psychopathic group, the distinction between intent-to-treat and completers was crucial. Prototypical psychopathic offenders were significantly more likely to drop out, but completers appeared to proceed through the different phases of treatment in much the same way as the other groups. Clearly, more research is needed to elucidate treatment interfering mechanisms and their associated patient characteristics, particularly for the prototypical psychopathic group. Developing therapeutic strategies to improve treatment compliance is a necessary step in the development of specialized treatment programs for these difficult patients

Traumatische rouw bij nabestaanden na geweldsmisdrijven: diagnostiek en behandeling

Volgens het Nederlandse Centraal Bureau voor de Statistiek (2022) is 5,4% van alle overlijdens in Nederland in 2020 (9030 van 168.678) het uiteindelijke gevolg van uitwendige (“niet-natuurlijke”) doodsoorzaken. Het gaat hierbij om ongevallen (71,2%, 6433), zelfdoding (20,2%, 1823) moord en doodslag (1,2%, 107), overig geweld (7,1%, 639) en gebeurtenissen opzet onbekend (0,3%, 28). Bij nabestaanden is het risico op traumatische rouw na een dergelijk ’traumatisch’ verlies sterk verhoogd vergeleken met een verlies door ziekte van een dierbare. Herkenning en behandeling van traumatische rouw, die veelal gepaard gaat met intens lijden en beperkingen in functioneren, is van groot belang; behandeling kan leiden tot aanzienlijke klachtenreductie, ook langere tijd na het verlies

Traumatische rouw bij nabestaanden na geweldsmisdrijven: diagnostiek en behandeling

Volgens het Nederlandse Centraal Bureau voor de Statistiek (2022) is 5,4% van alle overlijdens in Nederland in 2020 (9030 van 168.678) het uiteindelijke gevolg van uitwendige (“niet-natuurlijke”) doodsoorzaken. Het gaat hierbij om ongevallen (71,2%, 6433), zelfdoding (20,2%, 1823) moord en doodslag (1,2%, 107), overig geweld (7,1%, 639) en gebeurtenissen opzet onbekend (0,3%, 28). Bij nabestaanden is het risico op traumatische rouw na een dergelijk ’traumatisch’ verlies sterk verhoogd vergeleken met een verlies door ziekte van een dierbare. Herkenning en behandeling van traumatische rouw, die veelal gepaard gaat met intens lijden en beperkingen in functioneren, is van groot belang; behandeling kan leiden tot aanzienlijke klachtenreductie, ook langere tijd na het verlies

Clinical Appraisals of Individual Differences in Treatment Responsivity Among Patients With Psychopathy: A Consensual Qualitative Research Study

This study addressed which factors expert clinicians consider crucial in successful completion versus dropout in the mandatory forensic psychiatric treatment of psychopathic patients in the Netherlands. Eleven clinicians were interviewed about patient characteristics, treatment (provider) characteristics, and other factors they deemed associated with failure (transfer to another facility) or completion. The interviews were coded using the guidelines of Consensual Qualitative Research (CQR). Overall, extremely high scores on Psychopathy Checklist–Revised (PCL-R) Facets 1 (Deceitful Interpersonal Style) and 2 (Defective Affective Experience) were thought to impede treatment retention, particularly by its negative impact on motivation and therapeutic relationship. Older patients, those with a prosocial network, and/or patients with comorbid borderline traits appeared to fare better. Treatment success was deemed more likely when treatment goals and expectations are stipulated in a concrete fashion, when an extended and gradual resocialization trajectory is offered, and the treatment team is expert, cohesive, and stable.</p

Using MMPI–2–RF Correlates to Elucidate the PCL–R and Its Four Facets in a Sample of Male Forensic Psychiatric Patients

This study documents the associations between the MMPI-2-RF (Ben-Porath & Tellegen, 2008 ) scale scores and the Psychopathy Checklist Revised (PCL-R; Hare, 2003 ) facet scores in a forensic psychiatric sample. Objectives were to determine how the MMPI-2-RF scales might enhance substantive understanding of the nature of the 4 PCL-R facets and to discern possible implications for the treatment of psychopathic patients. A sample of 127 male forensic psychiatric offenders admitted to a Dutch forensic psychiatric hospital completed the PCL-R and the MMPI-2. Exploratory stepwise regression analyses assessed the prediction of the PCL-R total and its facet scores from MMPI-2-RF scales at its 3 hierarchical levels. Conceptually meaningful results emerged at each level of the MMPI-2-RF hierarchy, including several consistent differences between predictor sets across the facets. Interestingly, ideas of persecution (RC6) was a specific predictor of PCL-R Facet 2, a facet noted for its association with treatment failure. Results are compared and contrasted to the extant body of empirical work to date, and some tentative clinical implications are offered

Responding to the new International Classification of Diseases-11 prolonged grief disorder during the COVID-19 pandemic: a new bereavement network and three-tiered model of care

The field of bereavement research and care is at a tipping point. The introduction of prolonged grief disorder (PGD) in the International Classification of Diseases (ICD-11) has ignited clinical interest in this new disorder, along with debate over challenges in validating and implementing these new criteria. At the same time, the global COVID-19 pandemic has launched several local and international efforts to provide urgent support and comfort for individuals and communities suffering from grief. Recently, grief experts have called for a collective response to these complicated bereavements and possible increase in PGD due to COVID-19. Here we outline a new European network that aims to unite a community of grief researchers and clinicians to provide accessible, evidence-based support particularly during times of unprecedent crisis. The Bereavement Network Europe (BNE) has been developed with two main aims. Firstly, to develop expert agreed, internationally acceptable guidelines for bereavement care through a three-tiered approach. Secondly, to provide a platform for researchers and clinicians to share knowledge, collaborate, and develop consensus protocols to facilitate the introduction of PGD to diverse stakeholders. This article outlines the current status and aims of the BNE along with the plans for upcoming network initiatives and the three-tiered bereavement care guidelines in response to the COVID-19 pandemic. Keywords: Bereavement network Europe; COVID-19; ICD-11; Prolonged grief disorder; Three-tiered bereavement care