4 research outputs found
Molecular Epidemiology of Pathogenic Hantaviruses in the Far East of Russia, 2015-2018
Human pathogenic hantaviruses, belonging to the family Hantaviridae, genus Orthohantavirus, are disseminated worldwide and cause hemorrhagic fever with renal syndrome (HFRS) in Europe and Asia. In the Far East of Russia Hantaan (HTNV), Amur (AMRV) and Seoul (SEOV) viruses are etiologic agent of HFRS, while the human pathogenic potential of other seven hantaviruses, circulating in this region, has not been researched adequately yet. Objective of the study was genetic identification of hantaviruses, associated with HFRS in the Far East of Russia during 2015-2018. Materials and methods. Blood samples of 64 HFRS patients from Jewish Autonomous Region, Khabarovsk and Primorsky Territories were analyzed for hantavirus RNA using reverse transcription polymerase chain reaction. Results and discussion. A total of 19 viral RNA isolates from HFRS patients were genetically typed. Etiologic agents of HFRS were three pathogenic hantaviruses: HTNV (13 isolates), AMRV (3 isolates), SEOV (3 isolates). Three genetic lineages were identified among HTNV, two lineages among AMRV One genetic variant of SEOV virus was identified among HFRS patients, which was more close to the strains from South-Eastern Asia than to those from the neighboring countries
Hantavirus Associated with Hemorrhagic Fever with Renal Syndrome Outbreak in the Saratov Region in 2019
Pathogenic hantaviruses, belonging to the family Hantaviridae, genus Orthohantavirus, are widely spread in many regions of the world and cause hemorrhagic fever with renal syndrome (HFRS) in Europe and Asia. In the European Russia, the most active HFRS foci are located in the optimum habitat area of natural reservoir of the Puumala virus (PUUV), bank voles (Myodes glareolus), – in the Middle Volga and Cis-Urals. The largest number of cases of HFRS was registered in the Volga Federal District. In 2019, an outbreak of HFRS was registered among the residents of Saratov and the Saratov Region, the number of cases was 2702. Objective of the study was genetic identification of hantaviruses from HFRS patients and rodent carriers and phylogenetic analysis of full-size genomes from natural hosts during HFRS outbreak in the Saratov Region, 2019. Materials and methods. Blood samples of 8 HFRS patients from Saratov and 3 lung samples of bank voles captured in the suburb of Saratov were analyzed using reverse transcription polymerase chain reaction, followed by sequencing and phylogenetic analysis. Results and discussion. A total of 6 viral RNA isolates from HFRS patients were genetically typed, full-length RNA-isolate genomes were obtained for 3 natural carriers. Our data indicate that PUUV virus was associated with HFRS outbreak in Saratov. Genetic analysis revealed that the virus belonged to RUS lineage of PUUV, related most closely to strains from Udmurtia and Tatarstan and differed from strains circulating in the territory of Bashkortostan and Samara Region