Spo0J and SMC are required for normal chromosome segregation in Staphylococcus aureus.

Bacterial chromosome segregation is an essential cellular process that is particularly elusive in spherical bacteria such as the opportunistic human pathogen Staphylococcus aureus. In this study, we examined the functional significance of a ParB homologue, Spo0J, in staphylococcal chromosome segregation and investigated the role of the structural maintenance of chromosomes (SMC) bacterial condensin in this process. We show that neither spo0J nor smc is essential in S. aureus; however, their absence causes abnormal chromosome segregation. We demonstrate that formation of complexes containing Spo0J and SMC is required for efficient S. aureus chromosome segregation and that SMC localization is dependent on Spo0J. Furthermore, we found that cell division and cell cycle progression are unaffected by the absence of spo0J or smc. Our results verify the role of Spo0J and SMC in ensuring accurate staphylococcal chromosome segregation and also imply functional redundancy or the involvement of additional mechanisms that might contribute to faithful chromosome inheritance

Cell shape-independent FtsZ dynamics in synthetically remodeled bacterial cells.

FtsZ is the main regulator of bacterial cell division. It has been implicated in acting as a scaffolding protein for other division proteins, a force generator during constriction, and more recently, as an active regulator of septal cell wall production. FtsZ assembles into a heterogeneous structure coined the Z-ring due to its resemblance to a ring confined by the midcell geometry. Here, to establish a framework for examining geometrical influences on proper Z-ring assembly and dynamics, we sculpted Escherichia coli cells into unnatural shapes using division- and cell wall-specific inhibitors in a micro-fabrication scheme. This approach allowed us to examine FtsZ behavior in engineered Z-squares and Z-hearts. We use stimulated emission depletion (STED) nanoscopy to show that FtsZ clusters in sculpted cells maintain the same dimensions as their wild-type counterparts. Based on our results, we propose that the underlying membrane geometry is not a deciding factor for FtsZ cluster maintenance and dynamics in vivo

Beamforming Design for Active RIS-Aided Over-the-Air Computation

Over-the-air computation (AirComp) is emerging as a promising technology for wireless data aggregation. However, its performance is hampered by users with poor channel conditions. To mitigate such a performance bottleneck, this paper introduces an active reconfigurable intelligence surface (RIS) into the AirComp system. Specifically, we begin by exploring the ideal RIS model and propose a joint optimization of the transceiver design and RIS configuration to minimize the mean squared error (MSE) between the target and estimated function values. To manage the resultant tri-convex optimization problem, we employ the alternating optimization (AO) technique to decompose it into three convex subproblems, each solvable optimally. Subsequently, we investigate two specific cases and analyze their respective asymptotic performance to reveal the superiority of the active RIS in mitigating the MSE relative to its passive counterpart. Lastly, we adapt our transceiver and RIS configuration design to account for the self-interference of the active RIS. To handle the resultant highly non-convex problem, we further devise a two-layer AO framework. Simulation results demonstrate the superiority of the active RIS in enhancing AirComp performance compared to its passive counterpart

Serum Neurofilament Light Protein as a Marker for Diffuse Axonal Injury: Results from a Case Series Study

Diffuse axonal injury (DAI) is an important cause of morbidity in patients with traumatic brain injury (TBI). There is currently no simple and reliable technique for early identification of patients with DAI, or to prognosticate long-term outcome in this patient group. In the present study, we examined acute serum concentrations of neurofilament light (NFL) in nine patients with severe TBI and DAI using a novel ultrasensitive single molecule array (Simoa) assay. The relationships between the NFL concentrations and MRI in the acute stage as well as clinical outcome and magnetic resonance diffusion tensor imaging (MR-DTI) parameters at 12 months were analyzed. We found that the mean NFL concentrations among the patients displayed a 30-fold increase compared with controls, and that NFL completely discriminated between the patients and controls. We also found a relationship between serum NFL and MR-DTI parameters, with higher NFL concentrations in patients with higher trace (R2 = 0.79) and lower fractional anisotropy (FA) (R 2 = 0.83). These results suggest that serum NFL may be a valuable blood biomarker for TBI, reflecting the severity of DAI

Decentralized Beamforming Design for Intelligent Reflecting Surface-enhanced Cell-free Networks

Cell-free networks are considered as a promising distributed network architecture to satisfy the increasing number of users and high rate expectations in beyond-5G systems. However, to further enhance network capacity, an increasing number of high-cost base stations (BSs) are required. To address this problem and inspired by the cost-effective intelligent reflecting surface (IRS) technique, we propose a fully decentralized design framework for cooperative beamforming in IRS-aided cell-free networks. We first transform the centralized weighted sum-rate maximization problem into a tractable consensus optimization problem, and then an incremental alternating direction method of multipliers (ADMM) algorithm is proposed to locally update the beamformer. The complexity and convergence of the proposed method are analyzed, and these results show that the performance of the new scheme can asymptotically approach that of the centralized one as the number of iterations increases. Results also show that IRSs can significantly increase the system sum-rate of cell-free networks and the proposed method outperforms existing decentralized methods.Comment: 5 pages, 6 figure

Stable isotope records for the last 10 000 years from Okshola cave (Fauske, northern Norway) and regional comparisons

The sensitivity of terrestrial environments to past changes in heat transport is expected to be manifested in Holocene climate proxy records on millennial to seasonal timescales. Stalagmite formation in the Okshola cave near Fauske (northern Norway) began at about 10.4 ka, soon after the valley was deglaciated. Past monitoring of the cave and surface has revealed stable modern conditions with uniform drip rates, relative humidity and temperature. Stable isotope records from two stalagmites provide time-series spanning from c. 10 380 yr to AD 1997; a banded, multi-coloured stalagmite (Oks82) was formed between 10 380 yr and 5050 yr, whereas a pristine, white stalagmite (FM3) covers the period from ~7500 yr to the present. The stable oxygen isotope (δ<sup>18</sup>O<sub>c</sub>), stable carbon isotope (δ<sup>13</sup>C<sub>c</sub>), and growth rate records are interpreted as showing i) a negative correlation between cave/surface temperature and δ<sup>18</sup>O<sub>c</sub>, ii) a positive correlation between wetness and δ<sup>13</sup>C<sub>c</sub>, and iii) a positive correlation between temperature and growth rate. Following this, the data from Okshola show that the Holocene was characterised by high-variability climate in the early part, low-variability climate in the middle part, and high-variability climate and shifts between two distinct modes in the late part. <br><br> A total of nine Scandinavian stalagmite δ<sup>18</sup>O<sub>c</sub> records of comparable dating precision are now available for parts or most of the Holocene. None of them show a clear Holocene thermal optimum, suggesting that they are influenced by annual mean temperature (cave temperature) rather than seasonal temperature. For the last 1000 years, δ<sup>18</sup>O<sub>c</sub> values display a depletion-enrichment-depletion pattern commonly interpreted as reflecting the conventional view on climate development for the last millennium. Although the δ<sup>18</sup>O<sub>c</sub> records show similar patterns and amplitudes of change, the main challenges for utilising high-latitude stalagmites as palaeoclimate archives are i) the accuracy of the age models, ii) the ambiguity of the proxy signals, and iii) calibration with monitoring data

Cell shape independent FtsZ dynamics in synthetically remodeled cells

The FtsZ protein is a key regulator of bacterial cell division. It has been implicated in acting as a scaffolding protein for other division proteins, being a force generator during constriction, and more recently, as an active regulator of septal cell wall production. During an early stage of the division cycle, FtsZ assembles into a heterogeneous structure coined the “Z-ring” due to its resemblance to a ring confined by the midcell geometry. While in vitro experiments on supported lipid bilayers have shown that purified FtsZ can self-organize into a swirling ring roughly the diameter of a bacterial cell, it is not known how, and if, membrane curvature affects FtsZ assembly and dynamics in vivo . To establish a framework for examining geometrical influences on proper Z-ring assembly and dynamics, we sculptured Escherichia coli cells into unnatural shapes, such as squares and hearts, using division- and cell wall-specific inhibitors in a micro fabrication scheme. This approach allowed us to examine FtsZ behavior in engineered “Z-squares” and “Z-hearts”, and in giant cells up to 50 times their normal volume. Quantification of super-resolution STimulated Emission Depletion (STED) nanoscopy data showed that FtsZ densities in sculptured cells maintained the same dimensions as their wild-type counterparts. Additionally, time-resolved fluorescence measurements revealed that FtsZ dynamics were generally conserved in a wide range of cell shapes. Based on our results, we conclude that the underlying membrane environment is not a deciding factor for FtsZ filament maintenance and treadmilling in vivo