42 research outputs found
Transcriptome profiling of lentil (Lens culinaris) through the first 24 hours of Ascochyta lentis infection reveals key defence response genes
The influence of BTH and arbutin on PAL activity and phenolic compounds content in cucumber leaves infected with Pseudomonas syringae pv. lachrymans
Koncentracija selena i aktivnost glutationperoksidazy v krovi ljudejj prozhivajushhikh v Lodzi i ejo okrestnostjakh
Involvement of salicylic acid in defense response of BTH- and arbutin-treated Cucumis sativus against Pseudomonas syringe pv. lachrymans
Selected biochemical parameters of macrophytes applied in the Model Biofiltering System (MBS)
Antioxidant profiles and selected parameters of primary metabolism in Physalis ixocarpa hairy roots transformed by two Agrobacterium rhizogenes strains
We compared the biochemical profiles of Physalis ixocarpa hairy roots transformed with Agrobacterium rhizogenes
ATCC and A4 strains with non-transformed root cultures. The studied clones of A4- and ATCC-induced
hairy roots differed significantly; the latter showed greater growth potential and greater ability to produce secondary
metabolites (tropane alkaloids) and to biotransform hydroquinone to arbutin. We compared glucose content,
alanine and aspartate aminotransferase activity, and L-phenylalanine ammonia-lyase activity. We analyzed
markers of prooxidant/antioxidant homeostasis: catalase, ascorbate peroxidase, oxidase, glutathione peroxidase
and transferase activity, and the levels of ascorbate, glutathione, tocopherol and lipid peroxidation. We found
that transformation induced strain-specific regulation, including regulation based on redox signals, determining
the rate of allocation of carbon and nitrogen resources to secondary metabolism pathways. Our results provide
evidence that A. rhizogenes strain-specific modification of primary metabolites contributed to regulation of secondary
metabolism and could determine the ability of P. ixocarpa hairy root clones to produce tropane alkaloids
and to convert exogenously applied hydroquinone to pharmaceutically valuable arbutin. Of the studied
parameters, glucose content, L-phenylalanine ammonia-lyase activity and alanine aminotransferases activity may
be indicators of the secondary metabolite-producing potential of different P. ixocarpa hairy root clones