1 research outputs found
Comparative DNA Cytometry of Primary and Recurrent Soft Tissue Sarcomas
The goal of comparative investigation was to reveal the distinctive features of the DNA content and cell distribution in the phases of the cell cycle of recurrent STS. DNA cytometry in the tumor tissue of 30 primary soft tissue sarcomas (t2a-2bn0M0) and 30 STS recurrences (t2-3n0M1) was carried out using the method of flow cytofluorometry. the tumor ploidy and cell distribution in the cell cycle phases were analyzed. Results. A number of differences in the DNA cytometric parameters of primary and recurrent STS have been revealed, they include: an increase in the proportion of aneuploid tumors in case of recurrence, the number of tumors with DNA index within the mitotic cycle, an increase in the proportion of cells in G2+M- phase of diploid and aneuploidy tumors and a decrease in S- phase of aneuploid ones. It has been shown that with a G2 differentiation degree, the proportion of cells in G2+M, S- and IP of recurrent STS is significantly lower than the primary parameters. An increase in the proportion of cells in G2+M- phase and a decrease in the rate of proliferation of recurrent STS, depending on the stage, are shown only in case of stage III. Conclusion. The revealed features of DNA content and cell cycle of tumor cells of soft tissue sarcomas will allow to approach to understanding of biological bases of recurrence of this malignant disease.Π¦Π΅Π»ΡΡ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π±ΡΠ»ΠΎ Π²ΡΡΠ²ΠΈΡΡ Π² ΡΡΠ°Π²Π½ΠΈΡΠ΅Π»ΡΠ½ΠΎΠΌ Π°ΡΠΏΠ΅ΠΊΡΠ΅ ΠΎΡΠ»ΠΈΡΠΈΡΠ΅Π»ΡΠ½ΡΠ΅ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΠΠΠ ΠΈ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΠΎ ΡΠ°Π·Π°ΠΌ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠΊΠ»Π° ΡΠ΅ΡΠΈΠ΄ΠΈΠ²Π½ΡΡ
Π‘ΠΠ’. ΠΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΏΡΠΎΡΠΎΡΠ½ΠΎΠΉ ΡΠΈΡΠΎΡΠ»ΡΠΎΡΠΈΠΌΠ΅ΡΡΠΈΠΈ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΠΠ-ΡΠΈΡΠΎΠΌΠ΅ΡΡΠΈΡ Π² ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΠΎΠΉ ΡΠΊΠ°Π½ΠΈ 30 ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΡΡ
ΡΠ°ΡΠΊΠΎΠΌ ΠΌΡΠ³ΠΊΠΈΡ
ΡΠΊΠ°Π½Π΅ΠΉ (T2a-2bN0M0) ΠΈ 30 β ΡΠ΅ΡΠΈΠ΄ΠΈΠ²ΠΎΠ² Π‘ΠΠ’ (t2-3n0M1). Π°Π½Π°Π»ΠΈΠ·ΠΈΡΠΎΠ²Π°Π»ΠΈ ΠΏΠ»ΠΎΠΈΠ΄Π½ΠΎΡΡΡ ΠΎΠΏΡΡ
ΠΎΠ»ΠΈ ΠΈ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΠ΅ ΠΊΠ»Π΅ΡΠΎΠΊ ΠΏΠΎ ΡΠ°Π·Π°ΠΌ ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠΊΠ»Π°. ΡΠ΅Π·ΡΠ»ΡΡΠ°ΡΡ. ΠΡΡΠ²Π»Π΅Π½ ΡΡΠ΄ ΡΠ°Π·Π»ΠΈΡΠΈΠΉ ΠΠΠ-ΡΠΈΡΠΎΠΌΠ΅ΡΡΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΏΠΎΠΊΠ°Π·Π°ΡΠ΅Π»Π΅ΠΉ ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΡΡ
ΠΈ ΡΠ΅ΡΠΈΠ΄ΠΈΠ²Π½ΡΡ
Π‘ΠΠ’, ΠΊΠΎΡΠΎΡΡΠ΅ Π·Π°ΠΊΠ»ΡΡΠ°ΡΡΡΡ: Π² ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠΈ Π΄ΠΎΠ»ΠΈ Π°Π½Π΅ΡΠΏΠ»ΠΎΠΈΠ΄Π½ΡΡ
ΠΎΠΏΡΡ
ΠΎΠ»Π΅ΠΉ ΠΏΡΠΈ ΡΠ΅ΡΠΈΠ΄ΠΈΠ²Π°Ρ
, ΡΠΈΡΠ»Π° ΠΎΠΏΡΡ
ΠΎΠ»Π΅ΠΉ Ρ ΠΠΠΠ Π² ΠΏΡΠ΅Π΄Π΅Π»Π°Ρ
ΠΌΠΈΡΠΎΡΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΡΠΈΠΊΠ»Π°, ΡΠ²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ Π΄ΠΎΠ»ΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ Π² G2+M- ΡΠ°Π·Π΅ Π΄ΠΈΠΏΠ»ΠΎΠΈΠ΄Π½ΡΡ
ΠΈ Π°Π½Π΅ΡΠΏΠ»ΠΎΠΈΠ΄Π½ΡΡ
ΠΎΠΏΡΡ
ΠΎΠ»Π΅ΠΉ ΠΈ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ Π² S-ΡΠ°Π·Π΅ Π°Π½Π΅ΡΠΏΠ»ΠΎΠΈΠ΄Π½ΡΡ
. ΠΠΎΠΊΠ°Π·Π°Π½ΠΎ, ΡΡΠΎ ΠΏΡΠΈ ΡΡΠ΅ΠΏΠ΅Π½ΠΈ Π΄ΠΈΡΡΠ΅ΡΠ΅Π½ΡΠΈΡΠΎΠ²ΠΊΠΈ G2 Π΄ΠΎΠ»Ρ ΠΊΠ»Π΅ΡΠΎΠΊ Π² ΡΠ°Π·Π°Ρ
G2+M, S- ΠΈ ΠΠ ΡΠ΅ΡΠΈΠ΄ΠΈΠ²Π½ΡΡ
Π‘ΠΠ’ Π·Π½Π°ΡΠΈΠΌΠΎ Π½ΠΈΠΆΠ΅ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠΎΠ² ΠΏΠ΅ΡΠ²ΠΈΡΠ½ΡΡ
. Π£Π²Π΅Π»ΠΈΡΠ΅Π½ΠΈΠ΅ Π΄ΠΎΠ»ΠΈ ΠΊΠ»Π΅ΡΠΎΠΊ Π² G2+M-ΡΠ°Π·Π΅ ΠΈ ΡΠ½ΠΈΠΆΠ΅Π½ΠΈΠ΅ ΡΠ΅ΠΌΠΏΠΎΠ² ΠΏΡΠΎΠ»ΠΈΡΠ΅ΡΠ°ΡΠΈΠΈ ΡΠ΅ΡΠΈΠ΄ΠΈΠ²Π½ΡΡ
Π‘ΠΠ’ Π² Π·Π°Π²ΠΈΡΠΈΠΌΠΎΡΡΠΈ ΠΎΡ ΡΡΠ°Π΄ΠΈΠΈ ΠΏΠΎΠΊΠ°Π·Π°Π½Ρ ΡΠΎΠ»ΡΠΊΠΎ ΠΏΡΠΈ III ΡΡΠ°Π΄ΠΈΠΈ. ΠΡΠ²ΠΎΠ΄Ρ. ΠΡΡΠ²Π»Π΅Π½Π½ΡΠ΅ ΠΎΡΠΎΠ±Π΅Π½Π½ΠΎΡΡΠΈ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΡ ΠΠΠ ΠΈ ΠΏΠ°ΡΠ°ΠΌΠ΅ΡΡΠΎΠ² ΠΊΠ»Π΅ΡΠΎΡΠ½ΠΎΠ³ΠΎ ΡΠΈΠΊΠ»Π° ΠΎΠΏΡΡ
ΠΎΠ»Π΅Π²ΡΡ
ΠΊΠ»Π΅ΡΠΎΠΊ ΡΠ°ΡΠΊΠΎΠΌ ΠΌΡΠ³ΠΊΠΈΡ
ΡΠΊΠ°Π½Π΅ΠΉ ΠΏΠΎΠ·Π²ΠΎΠ»ΡΡ ΠΏΡΠΈΠ±Π»ΠΈΠ·ΠΈΡΡΡΡ ΠΊ ΠΏΠΎΠ½ΠΈΠΌΠ°Π½ΠΈΡ Π±ΠΈΠΎΠ»ΠΎΠ³ΠΈΡΠ΅ΡΠΊΠΈΡ
ΠΎΡΠ½ΠΎΠ² ΡΠ΅ΡΠΈΠ΄ΠΈΠ²ΠΈΡΠΎΠ²Π°Π½ΠΈΡ ΡΡΠΎΠ³ΠΎ Π·Π»ΠΎΠΊΠ°ΡΠ΅ΡΡΠ²Π΅Π½Π½ΠΎΠ³ΠΎ Π·Π°Π±ΠΎΠ»Π΅Π²Π°Π½ΠΈΡ