Identification and sequence determination of a novel double-stranded RNA mycovirus from the entopathogenic fungus Beauveria bassina

The final publication is available at Springer via https://doi.org/10.1007/s00705-014-2332-8.An isolate of the entomopathogenic fungus Beauveria bassiana was found to contain five double-stranded (ds) RNA elements ranging from 1.5 to more than 3 kbp. The complete sequence of the largest dsRNA element is described here. Analysis of the RdRp nucleotide sequence reveals its similarity to unclassified dsRNA elements, such as Alternaria longipes dsRNA virus 1, and its distant relationship to the RNA-dependent RNA polymerases of members of the family PartitiviridaePeer reviewedFinal Accepted Versio

Cu-Zn isotope constraints on the provenance of air pollution in Central Europe: using soluble and insoluble particles in snow and rime

Abstract not availableMartin Novak, Adela Sipkova, Vladislav Chrastny, Marketa Stepanova, Petra Voldrichova, Frantisek Veselovsky, Eva Prechova, Vladimir Blaha, Jan Curik, Juraj Farkas, Lucie Erbanova, Leona Bohdalkova, Jan Pasava, Jitka Mikova, Arnost Komarek, Michael Krachle

Zinc isotope systematics in snow and ice accretions in Central European mountains

Abstract not available.Petra Voldrichova, Vladislav Chrastny, Adela Sipkova, Juraj Farkas, Martin Novak, Marketa Stepanova, Michael Krachler, Frantisek Veselovsky, Vladimir Blaha, Eva Prechova, Arnost Komarek, Leona Bohdalkova, Jan Curik, Jitka Mikova, Lucie Erbanova, Petra Pacherov

Maternal Choline Supplementation Alters Basal Forebrain Cholinergic Neuron Gene Expression In The Ts65Dn Mouse Model Of Down Syndrome

Down syndrome (DS), trisomy 21, is marked by intellectual disability and a premature aging profile including degeneration of the basal forebrain cholinergic neuron (BFCN) projection system, similar to Alzheimer\u27s disease (AD). Although data indicate that perinatal maternal choline supplementation (MCS) alters the structure and function of these neurons in the Ts65Dn mouse model of DS and AD (Ts), whether MCS affects the molecular profile of vulnerable BFCNs remains unknown. We investigated the genetic signature of BFCNs obtained from Ts and disomic (2N) offspring of Ts65Dn dams maintained on a MCS diet (Ts+, 2N+) or a choline normal diet (ND) from mating until weaning, then maintained on ND until 4.4–7.5 months of age. Brains were then collected and prepared for choline acetyltransferase (ChAT) immunohistochemistry and laser capture microdissection followed by RNA extraction and custom-designed microarray analysis. Findings revealed upregulation of select transcripts in classes of genes related to the cytoskeleton (Tubb4b), AD (Cav1), cell death (Bcl2), presynaptic (Syngr1), immediate early (Fosb, Arc), G protein signaling (Gabarap, Rgs10), and cholinergic neurotransmission (Chrnb3) in Ts compared to 2N mice, which were normalized with MCS. Moreover, significant downregulation was seen in select transcripts associated with the cytoskeleton (Dync1h1), intracellular signaling (Itpka, Gng3, and Mlst8), and cell death (Ccng1) in Ts compared to 2N mice that was normalized with MCS. This study provides insight into genotype-dependent differences and the effects of MCS at the molecular level within a key vulnerable cell type in DS and AD