High Energy Emission Processes in OJ 287 during 2009 Flare

The broadband spectrum of a BL Lac object, OJ 287, from radio to $\gamma$-rays obtained during a major $\gamma$-ray flare detected by \emph{Fermi} in 2009 are studied to understand the high energy emission mechanism during this episode. Using a simple one-zone leptonic model, incorporating synchrotron and inverse Compton emission processes, we show that the explanation of high energy emission from X-rays to $\gamma$-rays, by considering a single emission mechanism, namely, synchrotron self-Compton (SSC) or external Compton (EC) requires unlikely physical conditions. However, a combination of both SSC and EC mechanisms can reproduce the observed high energy spectrum satisfactorily. Using these emission mechanisms we extract the physical parameters governing the source and its environment. Our study suggests that the emission region of OJ 287 is surrounded by a warm infrared (IR) emitting region of $\sim 250 \, K$. Assuming this region as a spherical cloud illuminated by an accretion disk, we obtain the location of the emission region to be $\sim 9 pc$. This supports the claim that the $\gamma$-ray emission from OJ 287 during the 2009 flare arises from a location far away from the central engine as deduced from millimeter-gamma ray correlation study and very long baseline array images.Comment: 22 pages, 7 figures, 1 table, accepted for publication in MNRA

Brightest Fermi-LAT Flares of PKS 1222+216: Implications on Emission and Acceleration Processes

We present a high time resolution study of the two brightest $\gamma$-ray outbursts from a blazar PKS 1222+216 observed by the \textit{Fermi} Large Area Telescope (LAT) in 2010. The $\gamma$-ray light-curves obtained in four different energy bands: 0.1--3, 0.1--0.3, 0.3--1 and 1--3 GeV, with time bin of 6 hr, show asymmetric profiles with a similar rise time in all the bands but a rapid decline during the April flare and a gradual one during the June. The light-curves during the April flare show $\sim 2$ days long plateau in 0.1--0.3 GeV emission, erratic variations in 0.3--1 GeV emission, and a daily recurring feature in 1--3 GeV emission until the rapid rise and decline within a day. The June flare shows a monotonic rise until the peak, followed by a gradual decline powered mainly by the multi-peak 0.1--0.3 GeV emission. The peak fluxes during both the flares are similar except in the 1--3 GeV band in April which is twice the corresponding flux during the June flare. Hardness ratios during the April flare indicate spectral hardening in the rising phase followed by softening during the decay. We attribute this behavior to the development of a shock associated with an increase in acceleration efficiency followed by its decay leading to spectral softening. The June flare suggests hardening during the rise followed by a complicated energy dependent behavior during the decay. Observed features during the June flare favor multiple emission regions while the overall flaring episode can be related to jet dynamics.Comment: 17 pages, 9 figures, 4 tables, accepted for publication in Ap

Weak Lensing Effect on CMB in the Presence of a Dipole Anisotropy

We investigate weak lensing effect on cosmic microwave background (CMB) in the presence of dipole anisotropy. The approach of flat-sky approximation is considered. We determine the functions $\sigma_0^2$ and $\sigma_2^2$ that appear in expressions of the lensed CMB power spectrum in the presence of a dipole anisotropy. We determine the correction to B-mode power spectrum which is found to be appreciable at low multipoles ($l$). However, the temperature and E-mode power spectrum are not altered significantly.Comment: 9 page

Scale Invariance as a Solution to the Cosmological Constant Problem

We show that scale invariance provides a solution to the fine tuning problem of the cosmological constant. We construct a generalization of the standard model of particle physics which displays exact quantum scale invariance. The matter action is invariant under global scale transformations in arbitrary dimensions. However the gravitational action breaks scale invariance explicitly. The scale symmetry is broken spontaneously in the matter sector of the theory. We show that the contribution to the vacuum energy and hence the cosmological constant is identically zero from the matter sector within the full quantum theory. However the gravitational sector may give non-zero contributions to the cosmological constant at loop orders. No fine tuning may be required at loop orders since the matter sector gives zero contribution to the cosmological constant. We also show that we do not require full scale invariance in order to constrain the vacuum energy from the matter sector. We only require invariance under pseudoscale transformations. Using this idea and motivated by the concept of unimodular gravity we propose an alternative model. In this case also we show that matter sector gives exactly zero contribution to the vacuum energy.Comment: 12 pages, no figure

Multi-wavelength Temporal Variability of the Blazar 3C 454.3 during 2014 Activity Phase

We present a multi-wavelength temporal analysis of the blazar 3C 454.3 during the high $\gamma$-ray active period from May-December, 2014. Except for X-rays, the period is well sampled at near-infrared (NIR)-optical by the \emph{SMARTS} facility and the source is detected continuously on daily timescale in the \emph{Fermi}-LAT $\gamma$-ray band. The source exhibits diverse levels of variability with many flaring/active states in the continuously sampled $\gamma$-ray light curve which are also reflected in the NIR-optical light curves and the sparsely sampled X-ray light curve by the \emph{Swift}-XRT. Multi-band correlation analysis of this continuous segment during different activity periods shows a change of state from no lags between IR and $\gamma$-ray, optical and $\gamma$-ray, and IR and optical to a state where $\gamma$-ray lags the IR/optical by $\sim$3 days. The results are consistent with the previous studies of the same during various $\gamma$-ray flaring and active episodes of the source. This consistency, in turn, suggests an extended localized emission region with almost similar conditions during various $\gamma$-ray activity states. On the other hand, the delay of $\gamma$-ray with respect to IR/optical and a trend similar to IR/optical in X-rays along with strong broadband correlations favor magnetic field related origin with X-ray and $\gamma$-ray being inverse Comptonized of IR/optical photons and external radiation field, respectively.Comment: 15 pages, 5 figures, 1 table, MNRAS accepte

Cosmological Perturbation Analysis in a Scale Invariant Model of Gravity

We consider a model for gravity that is invariant under global scale transformations. It includes one extra real scalar field coupled non-minimally to the gravity fields. In this model all the dimensionful parameters like the gravitational constant and the cosmological constant etc. are generated by a solution of the classical equations of motion which breaks scale invariance. In this paper we demonstrate the stability of such a solution against small perturbations in a flat FRW background by making a perturbative expansion around this solution and solving the resulting equations linear in the perturbations.Comment: 9 pages, 1 figur

Validation of Metabolic Alterations in Microscale Cell Culture Lysates Using Hydrophilic Interaction Liquid Chromatography (HILIC)-Tandem Mass Spectrometry-Based Metabolomics

By standard convention, in order to increase the efficacy of metabolite detection from cell culture lysates, metabolite extracts from a large quantity of cells are utilized for multiple reaction monitoring-based metabolomic studies. Metabolomics from a small number of cell extracts offers a potential economical alternative to increased cell numbers, in turn increasing the utility of cell culture-based metabolomics. However, the effect of reduced cell numbers on targeted metabolomic profiling is relatively unstudied. Considering the limited knowledge available of the feasibility and accuracy of microscale cell culture metabolomics, the present study analyzes differences in metabolomic profiles of different cell numbers of three pancreatic cancer cell lines. Specifically, it examines the effects of reduced cell numbers on metabolite profiles by obtaining extracts either directly from microscale culture plates or through serial dilution of increased numbers of cellular metabolite extracts. Our results indicate reduced cell numbers only modestly affect the number of metabolites detected (93% of metabolites detected in cell numbers as low as 104 cells and 97% for 105 cells), independent of the method used to obtain the cells. However, metabolite peak intensities were differentially affected by the reduced cell numbers, with some peak intensities inversely proportional to the cell numbers. To help eliminate such potential inverse relationships, peak intensities for increased cell numbers were excluded from the comparative analysis. Overall, metabolite profiles from microscale culture plates were observed to differ from the serial dilution samples, which may be attributable to the medium-to-cell-number ratios. Finally, findings identify perturbations in metabolomic profiling for cellular extracts from reduced cell numbers, which offer future applications in microscale metabolomic evaluations

Genomic Alterations in Mucins Across Cancers

The significance of mucins in cancers has led to the development of novel biomarkers and therapeutic agents against cancers. Despite significant advances in the understanding of mucins, systemic investigations into the role of mucins in cancer biology focusing particularly on the histological subtypes and stages, along with other variables, are yet to be carried out to discover potential novel functions and cancer-specific roles. Here, we investigated 11 mucin expressing cancers for DNA mutations, mRNA expression, copy number, methylation, and the impacts these genomic features may have on patient survival by utilizing The Cancer Genome Atlas dataset. We demonstrate that mucin DNA mutations have a significant rate, pattern, and impact on cancer patient survival depending on the tissue of origin. This includes a frequent T112P mutation in MUC1 that is seen in half of the pancreatic MUC1 mutations, as well as being present in other cancers. We also observed a very frequent MUC4 mutation at H4205, which correlated with survival outcomes in patients. Furthermore, we observed significant alterations in mucin mRNA expression in multiple tumor types. Our results demonstrate de novo expression of certain mucins in cancer tissues, including MUC21 in colorectal cancers. We observed a general decrease in promoter methylation for mucins, which correlated with decreased expression of many genes, such as MUC15 in kidney cancers. Lastly, several mucin gene loci demonstrated copy number increase in multiple histological subtypes. Thus, our study presents a comprehensive analysis of genomic alterations in mucins and their corresponding roles in cancer progression