The epigenetic control of E-box and Myc-dependent chromatin modifications regulate the licensing of lamin B2 origin during cell cycle

Recent genome-wide mapping of the mammalian replication origins has suggested the role of transcriptional regulatory elements in origin activation. However, the nature of chromatin modifications associated with such trans-factors or epigenetic marks imprinted on cis-elements during the spatio-temporal regulation of replication initiation remains enigmatic. To unveil the molecular underpinnings, we studied the human lamin B2 origin that spatially overlaps with TIMM 13 promoter. We observed an early G1-specific occupancy of c-Myc that facilitated the loading of mini chromosome maintenance protein (MCM) complex during subsequent mid-G1 phase rather stimulating TIMM 13 gene expression. Investigations on the Myc-induced downstream events suggested a direct interaction between c-Myc and histone methyltransferase mixed-lineage leukemia 1 that imparted histone H3K4me3 mark essential for both recruitment of acetylase complex HBO1 and hyperacetylation of histone H4. Contemporaneously, the nucleosome remodeling promoted the loading of MCM proteins at the origin. These chromatin modifications were under the tight control of active demethylation of E-box as evident from methylation profiling. The active demethylation was mediated by the Ten-eleven translocation (TET)-thymine DNA glycosylase-base excision repair (BER) pathway, which facilitated spatio-temporal occupancy of Myc. Intriguingly, the genome-wide 43% occurrence of E-box among the human origins could support our hypothesis that epigenetic control of E-box could be a molecular switch for the licensing of early replicating origins

Hydrodynamic signatures of stationary Marangoni-driven surfactant transport

We experimentally study steady Marangoni-driven surfactant transport on the interface of a deep water layer. Using hydrodynamic measurements, and without using any knowledge of the surfactant physico-chemical properties, we show that sodium dodecyl sulphate and Tergitol 15-S-9 introduced in low concentrations result in a flow driven by adsorbed surfactant. At higher surfactant concentration, the flow is dominated by the dissolved surfactant. Using Camphoric acid, whose properties are {\it a priori} unknown, we demonstrate this method's efficacy by showing its spreading is adsorption dominated

Nonlinear Dynamics of Nuclear-Electronic Spin Processes in Ferromagnets

Spin dynamics is considered in ferromagnets consisting of electron and nuclear subsystems interacting with each other through hyperfine forces. In addition, the ferromagnetic sample is coupled with a resonance electric circuit. Under these conditions, spin relaxation from a strongly nonequilibrium initial state displays several peculiarities absent for the standard set-up in studying spin relaxation. The main feature of the nonlinear spin dynamics considered in this communication is the appearance of ultrafast coherent relaxation, with characteristic relaxation times several orders shorter than the transverse relaxation time $T_2$. This type of coherent spin relaxation can be used for extracting additional information on the intrinsic properties of ferromagnetic materials and also can be employed for different technical applications.Comment: 1 file, 4 pages, RevTex, no figure

Spin waves in the (0,pi) and (0,pi,pi) ordered SDW states of the t-t' Hubbard model: Application to doped iron pnictides

Spin waves in (0,pi) and (0,pi,pi) ordered spin-density-wave (SDW) states of the t-t' Hubbard model are investigated at finite doping. In the presence of small t', these composite ferro-antiferromagnetic (F-AF) states are found to be strongly stabilized at finite hole doping due to enhanced carrier-induced ferromagnetic spin couplings as in metallic ferromagnets. Anisotropic spin-wave velocities, spin-wave energy scale of around 200meV, reduced magnetic moment, and rapid suppression of magnetic order with electron doping x (corresponding to F substitution of O atoms in La O_{1-x} F_x Fe As or Ni substitution of Fe atoms in Ba Fe_{2-x} Ni_x As_2) obtained in this model are in agreement with observed magnetic properties of doped iron pnictides.Comment: 13 pages, 3 figure