Identification of New Bioactive Compounds by Gc-Ms and Estimation of Physiological and Biological Activity of Kala Dhaman (Cenchrus setigerus)

ABSTRACT The present study was carried out to determine the possible bioactive components (sterols) in acetone extracts of Cenchrus setigerus using GC-MS analysis and in-vivo estimation of nitrogen assimilatory enzymes (NR, ALT, AST, GS, GDH and GOGAT) of seedlings and antibiotic activity of root extracts in various polar solvents of Cenchrus setigerus were done and antibiotic activity was tested against the G-ve bacteria including Proteus merabilis, Klebsiella pnemoniae, Agerobacterium tumefaciens and one fungi Aspergillus niger using disk diffusion method followed by determination of minimum inhibitory concentrations (MIC) by broth dilution method, against sensitive bacteria. Results reveal that various steroids from the above said plant were identified. The prevailing compounds (sterols) in the acetone extract of C. setigerus were 9,19-Cycloergost-24(28)-en-3-ol, 4,14-dimethyl-, acetate, (3β, 4α, 5α)-(14.39%), 2-Hexadecen-1-ol, 3,7, 11,15-tetramethyl-, [R-[R*,R*-(E)]]-(phytol) (1.27%), Cholest-4-en-3-one (2.36%) and β-Tocopherol (2.07%). All nitrogen assimilatory enzymes, GS showed highest activity followed by NR and highest antibiotic activity was exhibited by the isopropyl alcohol and chloroform extract against P. merabilis and K. pnemoniae after the GAA extract, The Glacial acetic acid extract was the most active, showing maximum Zone of inhibition (antibacterial effects) and A. tumefaciens (plant pathogen) was more sensitive to the extracts. Gentamycin and ketoconazole, the standard antibiotics were used to inhibiting these bacteria and fungi