Multimodal Biosensing on Paper-Based Platform Fabricated by Plasmonic Calligraphy Using Gold Nanobypiramids Ink

In this work, we design new plasmonic paper-based nanoplatforms with interesting capabilities in terms of sensitivity, efficiency, and reproducibility for promoting multimodal biodetection via Localized Surface Plasmon Resonance (LSPR), Surface Enhanced Raman Spectroscopy (SERS), and Metal Enhanced Fluorescence (MEF). To succeed, we exploit the unique optical properties of gold nanobipyramids (AuBPs) deposited onto the cellulose fibers via plasmonic calligraphy using a commercial pen. The first step of the biosensing protocol was to precisely graft the previously chemically-formed p-aminothiophenol@Biotin system, as active recognition element for target streptavidin detection, onto the plasmonic nanoplatform. The specific capture of the target protein was successfully demonstrated using three complementary sensing techniques. As a result, while the LSPR based sensing capabilities of the nanoplatform were proved by successive 13–18 nm red shifts of the longitudinal LSPR associated with the change of the surface RI after each step. By employing the ultrasensitive SERS technique, we were able to indirectly confirm the molecular identification of the biotin-streptavidin interaction due to the protein fingerprint bands assigned to amide I, amide III, and Trp vibrations. Additionally, the formed biotin-streptavidin complex acted as a spacer to ensure an optimal distance between the AuBP surface and the Alexa 680 fluorophore for achieving a 2-fold fluorescence emission enhancement of streptavidin@Alexa 680 on the biotinylated nanoplatform compared to the same complex on bare paper (near the plasmonic lines), implementing thus a novel MEF sensing nanoplatform. Finally, by integrating multiple LSPR, SERS, and MEF nanosensors with multiplex capability into a single flexible and portable plasmonic nanoplatform, we could overcome important limits in the field of portable point-of-care diagnostics

Portable Plasmonic Paper-Based Biosensor for Simple and Rapid Indirect Detection of CEACAM5 Biomarker via Metal-Enhanced Fluorescence

Rapid, simple, and sensitive analysis of relevant proteins is crucial in many research areas, such as clinical diagnosis and biomarker detection. In particular, clinical data on cancer biomarkers show great promise in forming reliable predictions for early cancer diagnostics, although the current analytical systems are difficult to implement in regions of limited recourses. Paper-based biosensors, in particular, have recently received great interest because they meet the criteria for point-of-care (PoC) devices; the main drawbacks with these devices are the low sensitivity and efficiency in performing quantitative measurements. In this work, we design a low-cost paper-based nanosensor through plasmonic calligraphy by directly drawing individual plasmonic lines on filter paper using a ballpoint pen filled with gold nanorods (AuNR) as the colloidal ink. The plasmonic arrays were further successively coated with negatively and positively charged polyelectrolyte layers employed as dielectric spacers to promote the enhancement of the emission of carboxyl-functionalized quantum dots (QD)—previously conjugated with specific antibodies—for indirect detection of the carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5). The efficiency, sensitivity, as well as the specificity of our portable nanosensor were validated by recording the luminescence of the QD@Ab complex when different concentrations of CEACAM5 were added dropwise onto the calligraphed plasmonic arrays

Dynamique réactionnelle dans des environnements restreints

Le but principal de ce travail était d'étudier les processus de transfert de proton intra-moléculaire et d'isomérisation à l'intérieur des nano-cavités moléculaires des cyclodextrines. Trois molécules photochromes ont été étudiées en utilisant la spectroscopie d'absorption UV-vis: le thioindigo, le salicylidene-aniline et le 2-(2',4'-dinitrobenzyl)pyridine. Dans des solutions aqueuses des solvants dans lesquels la molécule photochrome est soluble, déplacements de bandes d'absorption ont pu être associés à la formation des complexes d'inclusion avec les cyclodextrines. Dans une deuxième étape, pour répondre aux mêmes questions, à savoir l'influence d'un environnement restreint sur les mouvements moléculaires, notre travail a été consacré à l'étude du système a-cyclodextrine/4-méthyle-pyridine (aCD/4MP). L'idée initiale était d'utiliser la mesure de l'éclatement tunnel du groupement méthyle de 4MP comme une sonde quantitative sensible de la surface d'énergie potentielle (SEP) de la cavité de aCD. Cette voie a été suivie dans une première expérience de diffusion inélastique des neutrons sur un complexe aCD/4MP cristallin à basse température. Toutefois, pour ce système, une grande partie de notre travail a été dédiée à l'analyse de la transition de phase réversible liquide-solide découverte dans la solution de 4MP contenant différentes quantités de aCD, en augmentant la température dans le domaine 40 - 70 C.GRENOBLE1-BU Sciences (384212103) / SudocSudocFranceF

Chemiresistive/SERS dual sensor based on densely packed gold nanoparticles

Chemiresistors are a class of sensitive electrical devices capable of detecting (bio)chemicals by simply monitoring electrical resistance. Sensing based on surface enhanced Raman scattering (SERS) represents a radically different approach, in which molecules are optically detected according to their vibrational spectroscopic fingerprint. Despite different concepts are involved, one can find in the literature examples from both categories reporting sensors made of gold nanoparticles. The same building blocks appear because both sensor classes share a common principle: nanometric interparticle gaps are needed, for electron tunneling in chemiresistors, and for enhancing electromagnetic fields by plasmon coupling in SERS-based sensors. By exploiting such nano-gaps in self-assembled films of gold nanoparticles, we demonstrate the proof of concept of a dual electrical/optical sensor, with both chemiresistive and SERS capabilities. The proposed device is realized by self-assembling 15 nm gold nanoparticles into few micrometers-wide strips across commercially available interdigitated electrodes. The dual-mode operation of the device is demonstrated by the detection of a biologically relevant model analyte, 4-mercaptophenyl boronic acid