Supernumerary teeth in premolar and molar area on CBCT: a pictorial review.

Objective: to build a descriptive classification of premolar and molar supernumerary teeth (ST) when preparing the cone beam computed tomography (CBCT) report. The aim is also to share wide range of CBCT images in the open access publishing model. Material and methods: For our review we systematically searched for articles from PubMed with 1) free full texts on ST in molar and premolar area and using CBCT, and 2) articles providing with information on complications related with the presence of ST in molar and premolar area. We also added to our review studies providing with classic ST classifications in premolar and molar area. Results: We found 29 cases of ST, and we freely illustrated them with 84 figures. We separated our pictorial review in: 1) unilateral ST in the mandible, 2) unilateral ST in the maxilla, 3) unilateral undersized ST, 4) bilateral ST, 5) ST with additional features, and 6) cases with major hyperdontia. Conclusions: we build up the classification matrix for premolar and molar ST with 11 descriptors and 50 boxes. The descriptors were: 1) location if the ST crown in axial view, 2) vertical location of the cusp tip in relation with closest erupted tooth in coronal view, 3) shape, 4) distribution, 5) Position (in relation to normal tooth eruption) in sagittal view, 6) State of eruption of the ST in the sagittal view, 7) Follicle size measurement in sagittal view, 8) External root resorption of adjacent teeth by ST and its location in relation to the long axis of the involved tooth, 9) Internal resorption of ST, 10) Adjacent tooth complication, and 11) Damage to surrounding structures if ST removal. The open access figures from the literature illustrated 11 boxes. With our pictorial review we were able to illustrate 45 out of 50 boxes, and freely provide the readership with the most complete description of ST in premolar and molar area on CBCT than in previously published studies.Objective: to build a descriptive classification of premolar and molar supernumerary teeth (ST) when preparing the cone beam computed tomography (CBCT) report. The aim is also to share wide range of CBCT images in the open access publishing model.  Material and methods: For our review we systematically searched for articles from PubMed with 1) free full texts on ST in molar and premolar area and using CBCT, and 2) articles providing with information on complications related with the presence of ST in molar and premolar area. We also added to our review studies providing with classic ST classifications in premolar and molar area.  Results: We found 29 cases of ST, and we freely illustrated them with 84 figures. We separated our pictorial review in: 1) unilateral ST in the mandible, 2) unilateral ST in the maxilla, 3) unilateral undersized ST, 4) bilateral ST, 5) ST with additional features, and 6) cases with major hyperdontia. Conclusions: we build up the classification matrix for premolar and molar ST with 11 descriptors and 50 boxes. The descriptors were: 1) location if the ST crown in axial view, 2) vertical location of the cusp tip in relation with closest erupted tooth in coronal view, 3) shape, 4) distribution, 5) Position (in relation to normal tooth eruption) in sagittal view, 6) State of eruption of the ST in the sagittal view, 7) Follicle size measurement in sagittal view, 8) External root resorption of adjacent teeth by ST and its location in relation to the long axis of the involved tooth, 9) Internal resorption of ST, 10) Adjacent tooth complication, and 11) Damage to surrounding structures if ST removal. The open access figures from the literature illustrated 11 boxes. With our pictorial review we were able to illustrate 45 out of 50 boxes, and freely provide the readership with the most complete description of ST in premolar and molar area on CBCT than in previously published studies

Biofilms bactériens et médecine dentaire

peer reviewe

КПКТ-візуалізація надкомплектних зубів в ділянці премолярів і молярів: ілюстративний огляд

Objective: to build a descriptive classification of premolar and molar supernumerary teeth (ST) when preparing the cone beam computed tomography (CBCT) report. The aim is also to share wide range of CBCT images in the open access publishing model. Material and methods: For our review we systematically searched for articles from PubMed with 1) free full texts on ST in molar and premolar area and using CBCT, and 2) articles providing with information on complications related with the presence of ST in molar and premolar area. We also added to our review studies providing with classic ST classifications in premolar and molar area. Results: We found 29 cases of ST, and we freely illustrated them with 84 figures. We separated our pictorial review in: 1) unilateral ST in the mandible, 2) unilateral ST in the maxilla, 3) unilateral undersized ST, 4) bilateral ST, 5) ST with additional features, and 6) cases with major hyperdontia. Conclusions: we build up the classification matrix for premolar and molar ST with 11 descriptors and 50 boxes. The descriptors were: 1) location if the ST crown in axial view, 2) vertical location of the cusp tip in relation with closest erupted tooth in coronal view, 3) shape, 4) distribution, 5) Position (in relation to normal tooth eruption) in sagittal view, 6) State of eruption of the ST in the sagittal view, 7) Follicle size measurement in sagittal view, 8) External root resorption of adjacent teeth by ST and its location in relation to the long axis of the involved tooth, 9) Internal resorption of ST, 10) Adjacent tooth complication, and 11) Damage to surrounding structures if ST removal. The open access figures from the literature illustrated 11 boxes. With our pictorial review we were able to illustrate 45 out of 50 boxes, and freely provide the readership with the most complete description of ST in premolar and molar area on CBCT than in previously published studies.Мета: скласти описову класифікацію надкомплектних зубів (НКЗ) в ділянці премолярів та молярів для використання в описі конусно-променевих комп’ютерних томограм (КПКТ). Метою також є поширення значної кількості зображень КПКТ за моделлю відкритого доступу. Матеріали та методи: Для складання огляду ми провели пошук безкоштовних для перегляду повних статей на PubMed з інформацією про: 1)  КПКТ НКЗ у ділянці премолярів та молярів; 2) Ускладнення, пов’язані з наявністю НКЗ в ділянці молярів та премолярів. До огляду також були додані класифікації НКЗ в ділянці премолярів і молярів, представлені у інших дослідженнях. Результати: Нами було знайдено 29 клінічних випадків НКЗ, які ми проілюстрували 84-ма малюнками. Ми розділили наш ілюстративний огляд на: 1) Однобічні НКЗ на нижній щелепі; 2) Однобічні НКЗ на верхній щелепі; 3) Однобічні мікродонтичні НКЗ; 4) Двобічні НКЗ; 5) НКЗ з додатковими особливостями; 6) Випадки значної гіпердонтії. Висновки: Створено класифікаційну матрицю для НКЗ в ділянці премолярів і молярів з 11 дескрипторами та 50 блоками. Дескрипторами були: 1) розташування коронки НКЗ в аксіальній проєкції, 2) вертикальне розташування вершини горбка у відношенні до найближчого прорізаного зуба в корональній проєкції, 3) форма, 4) розподіл, 5) положення (у відношенні до положення нормально прорізаного зуба) в сагітальній проєкції, 6) Стан прорізування НКЗ в сагітальній проєкції, 7) Розмір фолікула в сагітальній проєкції, 8) Зовнішня резорбція коренів сусідніх зубів, спричинена НКЗ та його розташуванням у відношенні до довгої осі ураженого зуба, 9) внутрішня резорбція НКЗ, 10) ускладнення у сусідніх зубах, спричинені наявністю НКЗ, 11) ушкодження навколишніх анатомічних структур внаслідок видалення НКЗ. Малюнками з літератури, що знаходилася у відкритому доступі, було проілюстровано 11 блоків. У нашому ілюстративному огляді нам вдалося проілюструвати 45 з 50 блоків, і надати читачам вільний доступ до більш повного опису КПКТ НКЗ в ділянці премолярів та молярів, ніж у раніше опублікованих дослідженнях

Graft of autologous fibroblasts in gingival tissue in vivo after culture in vitro. Preliminary study on rats.

peer reviewedSeveral grafting techniques and guided tissue regeneration techniques (GTR) have been well-developed in periodontal surgery. However, these techniques could induce pain and side effects, such as a gingival recession during the healing period following the therapy. The graft of a small autologous connective tissue, using non-invasive surgical techniques could yield several benefits for the patients. Our preliminary study explores the feasibility of collecting healthy gingival tissues, culturing them in vitro to amplify rat gingival fibroblasts (RGF) and inoculating the obtained cells into autologous rat gingival tissues in vivo. Gingival tissues samples were cultured as explants as described by Freshney et al. and Adolphe. Confluent cells surrounding explants were detached after 7 d of culture from Petri dishes using 0.05% trypsin and designated "first transferred cells" (T1). At the third passage (T3), cells cultured as monolayer were either examined under microscopy--phase contrast, scanning, or transmission electron--or numerated after trypan blue exclusion test. Autologous RGF labelled with fluorochrome were inoculated at the vestibular and palatine site of gingival tissue close to the superior incisors. In this preliminary study, 12 Wistar rats were used; for each, 2 biopsies were dissected and fixed for phase contrast or fluorescence microscopy. On d 1, 3 and 7 after injection in rat gingival tissues, fluorochrome-labelled cells could be detected in all these

Effect of nicotine on rat gingival fibroblasts in vitro

Nicotine from 3 to 5 mM affects growth and survival rate of rat gingival fibroblasts in vitro. Ultrastructural analysis revealed dilated mitochondria and vacuolization in treated cells, suggestive of necrosis, but increased apoptosis was also revealed by cytometry. On the basis of this in vitro study, it appear that tobacco, through its component nicotine, may directly affect various functions of rat gingival fibroblast

Culture of Gingival Fibroblasts on Bioabsorbable Regenerative Materials in Vitro

peer reviewedBACKGROUND: The use of membranes in guided tissue regeneration (GTR) can limit the apical migration of gingival cells and favor the establishment of new attachment by periodontal ligament fibroblasts. However, gingival recession during healing following GTR has been described as a frequent complication. The purpose of this study was to determine if gingival fibroblasts are affected by the composition of the bioabsorbable membranes used in mucogingival surgery. METHODS: Two type of bioabsorbable regenerative materials were used as cell carriers. Wistar rat gingival fibroblasts (RGF) were obtained from attached gingiva, cut into small fragments, and placed in culture dishes. When confluent, cells were detached using trypsin and identified as "first transferred cells" (P1). At the third passage (P3), cell count, trypan blue exclusion test, acid phosphatase activity, DNA synthesis, phase contrast microscopy, and scanning electron microscopy were performed. The cells were then placed in wells containing the membranes and incubated for 72 hours. RESULTS: When examined under microscopy, the control wells (without membranes) showed one cell type with the elongated appearance characteristic of fibroblasts. The wells with membranes showed an altered cell morphology with a high proportion of cell fragments regardless of the type of membrane used. CONCLUSIONS: These results suggest that cell carrier membranes could affect RGF morphology and thus alter gingival tissue healing following GTR