Biocontrol of root and crown rot in tomatoes under greenhouse conditions using Trichoderma harzianum and Paenibacillus lentimorbus. Additional effect of solarization

Indexación: ScieloTrichoderma harzianum 650 (Th650) and Paenebacillus lentimorbus 629 (Pl629) selected earlier for their ability to control Rhizoctonia solani, Fusarium solani and F. oxysporum in vitro, were applied alone or combined with solarization (summer assay) and/or with methyl bromide (MeBr) (summer and winter assays) to a soil with a high inoculum level, for the control of tomato root rot caused by the complex F. oxysporum f. sp. lycopersici - Pyrenochaeta lycopersici - Rhizoctonia solani. Evaluations were also performed independently for root damage caused by P. lycopersici, and also for R. solani in the summer assay. MeBr decreased tomato root damage caused by the complex from 88.7% to 21.2% and from 78.4% to 35.7% in the summer and in the winter assay, respectively. None of the bio-controllers could replace MeBr in the winter assay, but Th650 and Pl629 reduced root damage caused by this complex in the summer assay. Treatments with bio-controllers were improved by their combination with solarization in this season. Independent evaluations showed that the positive control of Th650 towards R. solani and the lack of effect on P. lycopersici correlates well with the endochitinase pattern expressed by Th650 in response to these phytopathogens. Root damage caused by R. solani can be controlled at a similar level as it does MeBr in summer assays, thus representing an alternative to the use of this chemical fungicide for the control of this phytopathogen.Financial support: Fondecyt 1990785

DISEÑO DE UNA COLUMNA DE ABSORCIÓN EMPACADA CONSIDERANDO CUATRO TIPOS DE EMPAQUE Y APLICANDO MATLAB

In the present work, a packed bed absorption column is designed to recover certain amounts of ethanol contained in a gaseous stream. Four packing types (50-mm metal Hiflow® rings, 50-mm ceramic Pall® rings, 50-mm metal Top Pak® rings and 25-mm metal VSP® rings) are considered in order to select the most appropriate one in terms of column dimensions, pressure drop and mass-transfer results. Several design parameters were determined including column diameter (D), packing height (Z), overall mass-transfer coefficient (Km) and gas pressure drop (P/Z), as well as the overall number of gas-phase transfer units (NtOG), overall height of a gas-phase transfer unit (HtOG) and the effective surface area of packing (ah). The most adequate packing to use for this absorption system constitutes the 25-mm metal VSP® rings, since it provided the greatest values of Km (0.325 kmol/m3.s), and ah (169.57 m-1), as well as the lowest values of both Z (0.6 m) and HtOG (0.145 m), meaning that it will supply the higher mass-transfer conditions with the lowest column dimensions. The influence of both gas mixture (QG) and solvent (mL) feed flowrates on D, Z, Km, P/Z, NtOG and HtOG was also evaluated for the four packing considered. The design methodology was solved using computing software MATLAB® version 7.8.0.347 (R2009a) (Math Works, 2009), and also Microsoft Excel®.DEn el presente trabajo se diseña una columna de absorción empacada para recuperar ciertas cantidades de etanol contenido en una corriente gaseosa. Se consideran 4 tipos de empaques (anillos Hiflow® metálicos de 50 mm, anillos Pall® cerámicos de 50 mm, anillos Top Pak® metálicos de 50 mm, y anillos VSP® metálicos de 25 mm) con el fin de seleccionar el más apropiado en términos de dimensiones de la columna, caída de presión y resultados de transferencia de masa. Se determinaron varios parámetros de diseño incluyendo diámetro de la columna (D), altura del empaque (Z), coeficiente global de transferencia de masa (Km) y caída de presión gaseosa (P/Z), así como también el número total de unidades de transferencia den fase gaseosa (NtOG), altura total de unidades de transferencia en fase gaseosa (HtOG) y el área superficial efectiva del empaque (ah). El empaque más adecuado de usar en este sistema de absorción constituye los anillos VSP® metálicos de 25 mm, ya que suministra los mayores valores de Km (0.325 kmol/m3.s), y ah (169.57 m-1), así como también los menores valores de tanto Z (0.6 m) y HtOG (0.145 m), significando que suministrará las condiciones más altas de transferencia de masa con las menores dimensiones dela columna. La influencia de los caudales de alimentación de tanto la mezcla gaseosa (QG) y el solvente (mL) sobre D, Z, Km, DP/Z, NtOG y HtOG fue también evaluada para los cuatro tipos de empaques considerados. La metodología de diseño fue resuelta empleando el software MATLAB® versión 7.8.0.347 (R2009a) (Math Works, 2009), y también Microsoft Excel®

DISEÑO DE UNA COLUMNA DE ABSORCIÓN EMPACADA CONSIDERANDO CUATRO TIPOS DE EMPAQUE Y APLICANDO MATLAB

In the present work, a packed bed absorption column is designed to recover certain amounts of ethanol contained in a gaseous stream. Four packing types (50-mm metal Hiflow® rings, 50-mm ceramic Pall® rings, 50-mm metal Top Pak® rings and 25-mm metal VSP® rings) are considered in order to select the most appropriate one in terms of column dimensions, pressure drop and mass-transfer results. Several design parameters were determined including column diameter (D), packing height (Z), overall mass-transfer coefficient (Km) and gas pressure drop (P/Z), as well as the overall number of gas-phase transfer units (NtOG), overall height of a gas-phase transfer unit (HtOG) and the effective surface area of packing (ah). The most adequate packing to use for this absorption system constitutes the 25-mm metal VSP® rings, since it provided the greatest values of Km (0.325 kmol/m3.s), and ah (169.57 m-1), as well as the lowest values of both Z (0.6 m) and HtOG (0.145 m), meaning that it will supply the higher mass-transfer conditions with the lowest column dimensions. The influence of both gas mixture (QG) and solvent (mL) feed flowrates on D, Z, Km, P/Z, NtOG and HtOG was also evaluated for the four packing considered. The design methodology was solved using computing software MATLAB® version 7.8.0.347 (R2009a) (Math Works, 2009), and also Microsoft Excel®.DEn el presente trabajo se diseña una columna de absorción empacada para recuperar ciertas cantidades de etanol contenido en una corriente gaseosa. Se consideran 4 tipos de empaques (anillos Hiflow® metálicos de 50 mm, anillos Pall® cerámicos de 50 mm, anillos Top Pak® metálicos de 50 mm, y anillos VSP® metálicos de 25 mm) con el fin de seleccionar el más apropiado en términos de dimensiones de la columna, caída de presión y resultados de transferencia de masa. Se determinaron varios parámetros de diseño incluyendo diámetro de la columna (D), altura del empaque (Z), coeficiente global de transferencia de masa (Km) y caída de presión gaseosa (P/Z), así como también el número total de unidades de transferencia den fase gaseosa (NtOG), altura total de unidades de transferencia en fase gaseosa (HtOG) y el área superficial efectiva del empaque (ah). El empaque más adecuado de usar en este sistema de absorción constituye los anillos VSP® metálicos de 25 mm, ya que suministra los mayores valores de Km (0.325 kmol/m3.s), y ah (169.57 m-1), así como también los menores valores de tanto Z (0.6 m) y HtOG (0.145 m), significando que suministrará las condiciones más altas de transferencia de masa con las menores dimensiones dela columna. La influencia de los caudales de alimentación de tanto la mezcla gaseosa (QG) y el solvente (mL) sobre D, Z, Km, DP/Z, NtOG y HtOG fue también evaluada para los cuatro tipos de empaques considerados. La metodología de diseño fue resuelta empleando el software MATLAB® versión 7.8.0.347 (R2009a) (Math Works, 2009), y también Microsoft Excel®

Domain wall dynamics in an optical Kerr cavity

An anisotropic (dichroic) optical cavity containing a self-focusing Kerr medium is shown to display a bifurcation between static --Ising-- and moving --Bloch-- domain walls, the so-called nonequilibrium Ising-Bloch transition (NIB). Bloch walls can show regular or irregular temporal behaviour, in particular, bursting and spiking. These phenomena are interpreted in terms of the spatio-temporal dynamics of the extended patterns connected by the wall, which display complex dynamical behaviour as well. Domain wall interaction, including the formation of bound states is also addressed.Comment: 15 pages Tex file with 11 postscript figures. Resubmitted to Phys. Rev.

Validating Regulatory Sensory Processing Disorders Using the Sensory Profile and Child Behavior Checklist (CBCL 1 –5)

The objective was to validate Regulatory Sensory Processing Disorders’ criteria (DC:0-3R, 2005) using empirical data on the presence and severity of sensory modulation deficits and specific psychiatric symptoms in clinical samples. Sixty toddlers who attended a child mental health unit were diagnosed by a clinical team. The following two groups were created: toddlers with RSPD(N = 14) and those with ‘‘other diagnoses in Axis I/II of the DC:0-3R00(OD3R) (N = 46). Independently of the clinical process, parents completed the Infant Toddler Sensory Profile (as a checklist for sensory symptoms) and the Achenbach Behavior Checklist for ages 1/2–5 (CBCL 1/2–5). The scores from the two groups were compared. The results showed the following for the RSPD group: a higher number of affected sensory areas and patterns than in the OD3R group; a higher percentage of sensory deficits in specific sensory categories; and a higher severity of behavioral symptoms such as withdrawal, inattention, other externalizing problems and pervasive developmental problems in CBCL 1/2–5. The results confirmed our hypotheses by indicating a higher severity of sensory symptoms and identifying specific behavioral problems in children with RSPD. The results revealed convergent validity between the instruments and the diagnostic criteria for RSPD and supported the validity of RSPD as a unique diagnosis. The findings also suggested the importance of identifying sensory modulation deficits in order to develop an early intervention to enhance the sensory capacities of children who do not fully satisfy the criteria for some DSM-IV-TR disorders

Synthesis and Characterization of a Coagulating Agent from Plantain Peel Starch (Musa paradisiaca), as Coadjuvant in Water Treatment

Coagulation processes are widely used for water treatment, mainly with chemical coagulants. In this research, starch derived from a waste (unripe plantain peel, Musa paradisiaca) was used as a starting point for a chemical modifcation. Through acetylation, its chemical structure was modifed and characterized by infrared spectrophotometry, for its evaluation as a coadjuvant in coagulation operations to reduce the turbidity of raw water. Two experimental designs were developed to evaluate the incidence of modifed starch as the main coagulant, or in conjunction with a conventional coagulant (Al2(SO4)3), at diferent (Al2(SO4)3)/acetylated starch ratios, in jar-test experiments. In the frst experimental design, with the acetylated starch as the main coagulant, turbidity removal percentages reached 47.93% (average value, 41.18%). For the (Al2(SO4)3)/acetylated starch coagulation process, 98.91% turbidity removal was reached in the second experimental design (average value, 97.16%). The impact of starch chemical substitution degree and the (Al2(SO4)3)/acetylated starch ratio on the fnal turbidity obtained in the jar-tests was determined using ANOVA test. There was a great infuence of the chemical substitution degree and the concentration of acetylated starch utilized, when modifed starch was used as the main coagulant. For the second experimental design, the (Al2(SO4)3)/acetylated starch ratio had a greater incidence on the turbidity removal. Thus, modifed starch obtained from plantain peel waste is a promising coadjuvant material for water coagulation processes

Detritic deposits of periglacial origin under coluvial organic soil on a slope in Serra da Loba. (Galicia. Spain): characterization and genesis

[Resumen] En ciertas posiciones de las partes media e inferior de la ladera aparece bajo el suelo orgánico un material detrítico muy fragmentado y ordenado en lechos estratificados. Se estudian dos perfiles con estas características, se canografía su extensión en la ladera, y se interpreta su génesis. Estos derrubios tienen poca fracci6n fina entre ellos, son bastante frescos, yaparecen ordenados según la pendiente y dispuestos en lechos en los que se alternan fragmentos de pequeño tamaño con otros más gruesos con más escaso contenido en fracción fina. Los estudios morfológicos y granulométricos revelan claras discontinuidades en los perfiles, así como una baja clasificaci6n de este material detrítico. La composición mineralógica es menos reveladora por su escasa evolución, prácticamente la heredada del material de partida, con predominio de minerales poco alterables. Se hace una correspondencia estratigráfica de episodios de deposici6n en ambos perfiles y se interpreta el origen de este material detrítico como ligado al accionamiento periglaciar. La localizaci6n en la ladera de estos depósitos y su potencia están relacionados con el menor espesor de las líneas de piedras, indicativas de procesos de erosión posterior. Sin embargo estos depósitos están ausentes en las partes más bajas de la ladera que, en cambio, muestran ser las más propicias a la deposición, ya que presentan un mayor engrosamiento de los horizontes orgánicos de acumulaci6n y en ellas no aparecen indicios erosivos. Esta ausencia se interpreta por ser también estas zonas, bajas y abrigadas, las que podrían ser más favorables a una mayor cobertura vegetal en las condiciones periglaciares, que impediría la deposición de los derrubios.[Abstract] Stratified beds of highly fragmented detritic material under organic soil occur on medium and low parts ofthe slope. Two profiles with these features are studied, their extent on the slope is mapped and their genesis interpreted. Debris are quite fresh, with little fine fraction between them; they are arranged according to the slope and consist of alternating beds of little size fragments and others coarser and poor in fine fraction. Morphological and granulometric studies reveal clear discontinuities in the profiles and a poor soning of the detritic Ínaterials. Mineralogical composition is less meaningful because of its little evolution, almost inherited from parent material, with a predominance of hardly weatherable minerals. A stratigraphic correspondence is established of deposition episodes between both profiles, and the origin of the detritic material is related to periglacial dynamics. The position of these deposits on the slope and their thickness are inversely related to the thickness of stonelines which are indicative of subsequent erosive processes. Detritic deposits are absent in the lowest part of the slope, which on the other hand appear to be the most favourable to deposition as showed by the greatest thickness of organic horizons of accumulation, and the no occurrence of erosive signs. This absence is' interpreted as being caused by the presence of a more extensive vegetal cover, under periglacial conditions, in the lowest and more protected slope, so preventing debris deposition

Seismic effects in archaeological sites: Archaeoseismological cataloguing and quantification

The analysis of the damage caused by seismic events in archaeological sites provides significant information about ancient and historic earthquakes that can improve seismic hazard analysis. In this paper a methodological analysis to quantify the deformation of structural damage recorded in archaeological sites is proposed. This methodology focuses on the establishment of the seismic/non-seismic origin of the observed damage and consists of three phases: (a) identification and inventory of damage; (b) quantification of deformation; and (c) incorporation of the results to seismic catalogues. The first phase is the inventory and mapping of the damage according with the classification of archaeological effects of earthquakes (EAEs). The second phase is to quantify the deformation of damage by setting different parameters such as the direction of maximum horizontal strain (ey). This analysis is performed at different levels: for each of the recorded EAEs, for all the records of a particular category of EAEs, and finally for the complete archaeological site including all the categories of EAEs. The homogeneity of the results (damage orientation) allows us to establish the seismic origin of deformations. This methodology has been developed in the Roman site of Baelo Claudia (Cádiz, Spain) and has been applied to different historical earthquakes in the Iberian Peninsula. To calibrate the method, analyses on oriented damage were carried out immediately after the recent instrumental earthquakes of Lorca 2011 (5.1 Mw, Spain) and Emilia Romagna 2012 (5.9 Mw; Italy).The analysis of the damage caused by seismic events in archaeological sites provides significant information about ancient and historic earthquakes that can improve seismic hazard analysis. In this paper a methodological analysis to quantify the deformation of structural damage recorded in archaeological sites is proposed. This methodology focuses on the establishment of the seismic / non-seismic origin of the observed damage and consists of three phases: (a) identification and inventory of damage; (b) quantification of deformation; and (c) incorporation of the results to seismic catalogues. The first phase is the inventory and mapping of the damage according with the classification of archaeological effects of earthquakes (EAEs). The second phase is to quantify the deformation of damage by setting different parameters such as the direction of maximum horizontal strain (ey). This analysis is performed at different levels: for each of the recorded EAEs, for all the records of a particular category of EAEs, and finally for the complete archaeological site including all the categories of EAEs. The homogeneity of the results (damage orientation) allows us to establish the seismic origin of deformations. This methodology has been developed in the Roman site of Baelo Claudia (Cádiz, Spain) and has been applied to different historical earthquakes in the Iberian Peninsula. To calibrate the method, analyses on oriented damage were carried out immediately after the recent instrumental earthquakes of Lorca 2011 (5.1 Mw, Spain) and Emilia Romagna 2012 (5.9 Mw; Italy).Este trabajo ha sido financiado por los proyectos CGL2015-67169-P (QTECTSPAIN, USAL) y CATESI-07 (IGME). Es una contribución al INQUA TERPRO Project 1299 y a los grupos de trabajo QTECT-AEQUA y GQMAEQU

Plasma-induced polymerization as a tool for surface functionalization of polymer scaffolds for bone tissue engineering: an in vitro study

A commonly applied strategy in the field of tissue engineering (TE) is the use of temporary three-dimensional scaffolds for supporting and guiding tissue formation in various in vitro strategies and in vivo regeneration approaches. The interactions of these scaffolds with highly sensitive bioentities such as living cells and tissues primarily occur through the material surface. Hence, surface chemistry and topological features have principal roles in coordinating biological events at the molecular, cellular and tissue levels on timescales ranging from seconds to weeks. However, tailoring the surface properties of scaffolds with a complex shape and architecture remains a challenge in materials science. Commonly applied wet chemical treatments often involve the use of toxic solvents whose oddments in the construct could be fatal in the subsequent application. Aiming to shorten the culture time in vitro (i.e. prior the implantation of the construct), in this work we propose a modification of previously described bone TE scaffolds made from a blend of starch with polycaprolactone (SPCL). The modification method involves surface grafting of sulfonic or phosphonic groups via plasma-induced polymerization of vinyl sulfonic and vinyl phosphonic acid, respectively. We demonstrate herein that the presence of these anionic functional groups can modulate cell adhesion mediated through the adsorbed proteins (from the culture medium). Under the conditions studied, both vitronectin adsorption and osteoblast proliferation and viability increased in the order SPCL!sulfonic-grafted SPCL < phosphonic-grafted SPCL. The results revealed that plasmainduced polymerization is an excellent alternative route, when compared to the commonly used wet chemical treatments, for the surface functionalization of biodevices with complex shape and porosity.The authors acknowledge funding from EU Marie Curie Actions, Alea Jacta Est (MEST-CT-2004-008104) and the Portuguese Foundation for Science and Technology (FCT) (SFRH/BPD/34545/2007). This work was carried out under the scope of the European NoE EXPERTISSUES (NMP3-CT-2004-500283). The authors also acknowledge Dr. M.I. Santos and C. Serra for their assistance on the CLSM and XPS experiments

Sobre algunas plantas recolectadas por J.M.C. Lange en Galicia y zonas limítrofes

Con base en la revisión del Herbario de Johan Martin Christian Lange conservado en Copenhague (C), se comentan algunas de la plantas citadas por él mismo en sus diversas publicaciones así como en el manuscrito que envió a V. López Seoane. Se confirma la presencia de Saxifraga cuneifolia L. en la Península Ibérica mientras que tres taxa constituyen novedades provinciales, Tuberaria plantaginea (Willd.) Gallego, Anthyllis vulneraria L. subsp. iberica (W. Becker) Jalas ex Cullen y Carduus assoi (Willk.) Devesa & Talavera subsp. assoi. Se elimina del catálogo florístico español Saxifraga umbrosa L. y del gallego Avena brevis Roth y se hacen diversas consideraciones sobre otros táxones. Se lectotipifican 3 nombres (Ligusticum pyrenaeum Gou./var. legionensis Lange, Hydrocotyle vulgaris L. forma microphylla Lge. y Achillea millefolium β macrocephala Lge. )