Functional brain activity constrained by structural connectivity reveals cohort-specific features for serum neurofilament light chain

Background: Neuro-axonal brain damage releases neurofilament light chain (NfL) proteins, which enter the blood. Serum NfL has recently emerged as a promising biomarker for grading axonal damage, monitoring treatment responses, and prognosis in neurological diseases. Importantly, serum NfL levels also increase with aging, and the interpretation of serum NfL levels in neurological diseases is incomplete due to lack of a reliable model for age-related variation in serum NfL levels in healthy subjects. Methods: Graph signal processing (GSP) provides analytical tools, such as graph Fourier transform (GFT), to produce measures from functional dynamics of brain activity constrained by white matter anatomy. Here, we leveraged a set of features using GFT that quantified the coupling between blood oxygen level dependent signals and structural connectome to investigate their associations with serum NfL levels collected from healthy subjects and former athletes with history of concussions. Results: Here we show that GSP feature from isthmus cingulate in the right hemisphere (r-iCg) is strongly linked with serum NfL in healthy controls. In contrast, GSP features from temporal lobe and lingual areas in the left hemisphere and posterior cingulate in the right hemisphere are the most associated with serum NfL in former athletes. Additional analysis reveals that the GSP feature from r-iCg is associated with behavioral and structural measures that predict aggressive behavior in healthy controls and former athletes. Conclusions: Our results suggest that GSP-derived brain features may be included in models of baseline variance when evaluating NfL as a biomarker of neurological diseases and studying their impact on personality traits

FOREST CANOPY DENSITY ASSESSMENT USING HIGH RESOLUTION LISS-4 DATA IN YAMUNANAGAR DISTRICT, HARYANA

Forest plays an important role not only in providing ecological services but also economic goods to human beings. However, with increase in population there is a wide gap between demand and supply of these goods and services. This has lead to reduction in forest cover which needs to be taken care on regular time interval. To manage the existing forest area and also to increase the forest cover Forest Canopy Density (FCD) methodology is the main factor which was given by International Tropical timber Organization (ITTO). High resolution remote sensing LISS-4 data gives us chance to assess the quality of forest in terms of FCD as Rikimaru et al (1999) stated that FCD is one important parameter to assess forest cover quality. High resolution LISS-4 data analysis for FCD was never attempted before. Authors here attempted to assess the FCD utilizing methodology adopted by Rikimaru (1999), Huang (2001), Azizia (2008). The adopted methodology is one of the most efficient and cost effective way to derive the FCD. For this study Resourcesat-2 LISS-4 post monsoon data of year 2017 for Yamunanagar district was used to assess FCD within notified forest boundary. Notified forest boundaries at cadastral level prepared previously by Haryana Space Applications Centre (HARSAC) was used. The degree of forest canopy density is expressed in percentages: i.e. < 10% FCD (scrub land), 10–20% (Open Forest-I), 20–40% (Open Forest-II), 40–60% (Moderate Dense), 60–80% (Medium Dense) and > 80% (Highly Dense). Forest Canopy Density was based on three indices i.e. Advanced Vegetation Index (AVI), Bare Soil Index (BSI) and Canopy Shadow Index (CSI). Accuracy assessment was done based on ground data and comparison with Coterminous Google Earth imagery and it was found that the devised methodology has achieved overall accuracy of 93% with kappa coefficient of 0.9153. The result shows that maximum forest area in Yamunanagar district is in medium dense FCD category which is approximately 23948.08 acres. This study tells us that 24.2% of the total forest area is under scrub land and open forest which should be focussed for activities in working plan to increase the forest cover. This paper highlights the utility of high resolution satellite data for monitoring and management of forest and improvement in its quality. This attempt provided large scale (1 : 10,000) maps to the forest managers to better equip them in planning for afforestation, reforestation and rehabilitation of water logged areas, environment management and their future aspect

Integrating Association Mapping, Linkage Mapping, Fine Mapping with RNA Seq Conferring Seedling Vigor Improvement for Successful Crop Establishment in Deep Sown Direct-Seeded Rice

Background: Ongoing large-scale shift towards direct seeded rice (DSR) necessitates a convergence of breeding and genetic approaches for its sustenance and harnessing natural resources and environmental benefits. Improving seedling vigour remains key objective for breeders working with DSR. The present study aims to understand the genetic control of seedling vigour in deep sown DSR. Combined genome-wide association mapping, linkage mapping, fine mapping, RNA-sequencing to identify candidate genes and validation of putative candidate genes were performed in the present study. Results: Significant phenotypic variations were observed among genotypes in both F3:4:5 and BC2F2:3 populations. The mesocotyl length showed significant positive correlation with %germination, root and shoot length. The 881 kb region on chromosome 7 reported to be associated with mesocotyl elongation. RNA-seq data and RT-PCR results identified and validated seven potential candidate genes. The four promising introgression lines free from linkage drag and with longer mesocotyl length, longer root length, semi-dwarf plant height have been identified. Conclusion: The study will provide rice breeders (1) the pre breeding material in the form of anticipated DSR adapted introgression lines possessing useful traits and alleles improving germination under deep sown DSR field conditions (2) the base for the studies involving functional characterization of candidate genes. The development and utilization of improved introgression lines and molecular markers may play an important role in genomics-assisted breeding (GAB) during the pyramiding of valuable genes providing adaptation to rice under DSR. Our results offer a robust and reliable package that can contribute towards enhancing genetic gains in direct seeded rice breeding programs

Cells activated for wound repair have the potential to direct collective invasion of an epithelium.

Mechanisms regulating how groups of cells are signaled to move collectively from their original site and invade surrounding matrix are poorly understood. Here we develop a clinically relevant ex vivo injury invasion model to determine whether cells involved in directing wound healing have invasive function and whether they can act as leader cells to direct movement of a wounded epithelium through a three-dimensional (3D) extracellular matrix (ECM) environment. Similar to cancer invasion, we found that the injured cells invade into the ECM as cords, involving heterotypical cell-cell interactions. Mesenchymal cells with properties of activated repair cells that typically locate to a wound edge are present in leader positions at the front of ZO-1-rich invading cords of cells, where they extend vimentin intermediate filament-enriched protrusions into the 3D ECM. Injury-induced invasion depends on both vimentin cytoskeletal function and MMP-2/9 matrix remodeling, because inhibiting either of these suppressed invasion. Potential push and pull forces at the tips of the invading cords were revealed by time-lapse imaging, which showed cells actively extending and retracting protrusions into the ECM. This 3D injury invasion model can be used to investigate mechanisms of leader cell-directed invasion and understand how mechanisms of wound healing are hijacked to cause disease

Regulation of nitrate reductase level in pea: in vivo stability by ammonium

Ammonium, the end-product of nitrate-reduction, causes a marked increase in nitrate-dependent formation of nitrate reductase activity in pea shoot apices. The ammonium effect is mediated via a decrease in the rate of nitrate reductase decay. The increased stability of the enzyme in the presence of ammonium is indirect and depends upon protein synthesis. A regulatory role for ammonium-induced protein(s) is suggested

Effect of ammonium, sucrose and light on the regulation of nitrate reductase level in Pisum sativum

In shoot apices of 7-day-old dark-grown peas the addition of ammonium along with the inducer nitrate resulted in a more than two-fold increase in nitrate reductase activity. Individual amino acids, amides and amino-acid mixture could not replace the ammonium effect. Ammonium also stimulated NADH-glutamate dehydrogenase but not glucose-6-phosphate dehydrogenase. Sucrose caused a marked stimulation of nitrate reductase induction and showed synergistic effect with light. In presence of cordycepin and cycloheximide, induction of nitrate reductase was inhibited more if ammonium or sucrose was supplied along with the inducer. With actinomycin D, α-amanitin or chloramphenicol, no differential inhibition took place in presence of ammonium. The inhibition of enzyme activity by chloramphenicol and 3-(3,4-dichlorophenyl)-l,dimethyl urea was completely relieved by sucrose. Incorporation of 14C-lysine was markedly stimulated by sucrose, but was not affected by ammonium. The effect of sucrose and light on 14C-lysine incorporation was additive. Cordycepin and cycloheximide did not have any differential effect on 14C-lysine incorporation in the presence of ammonium as well as sucrose. The inhibition of 14C-lysine incorporation caused by chloramphenicol was relieved by sucrose. Sucrose also caused a marked increase in 3H-uridine incorporation but ammonium had no effect. Actinomycin D and cordycepin blocked the sucrose dependent increase in 3H-uridine incorporation. The results suggest that ammonium mediated stimulation may depend on a regulatory protein(s) synthesized in response to ammonium, whereas sucrose acts mainly by an overall increase in RNA and protein synthesis. The effect of light does not seem to be dependent on photosynthetic light reactions