Clinical Study Can Whole-Body Cryotherapy with Subsequent Kinesiotherapy Procedures in Closed Type Cryogenic Chamber Improve BASDAI, BASFI, and Some Spine Mobility Parameters and Decrease Pain Intensity in Patients with Ankylosing Spondylitis?

The present study investigated whether whole-body cryotherapy (WBC) procedures could potentially have more beneficial effects on index of BASDAI and BASFI, pain intensity, and spine mobility parameters: Ott test, modified Schober test, chest expansion in ankylosing spondylitis (AS) patients, than kinesiotherapy procedures used separately. AS patients were exposed to a cycle of WBC procedures lasting 3 minutes a day, with a subsequent 60 minutes of kinesiotherapy or 60 minutes of kinesiotherapy only, for 10 consecutive days excluding weekend. After the completion of the cycle of WBC procedures with subsequent kinesiotherapy in the AS patients, BASDAI index decreased about 40% in comparison with the input value, whereas in the group of patients who received only kinesiotherapy it decreased only about 15% in comparison with the input value. After the completion of the treatment in the WBC group, BASFI index decreased about 30% in comparison with the input value, whereas in the kinesiotherapy group it only decreased about 16% in comparison with the input value. The important conclusion was that, in WBC group with subsequent kinesiotherapy, we observed on average about twice better results than in the group treated only by kinesiotherapy

Mutations in the COL1A1 and COL1A2 genes associated with osteogenesis imperfecta (OI) types I or III

Although over 85% of osteogenesis imperfecta (OI) cases are associated with mutations in the procollagen type I genes (COL1A1 or COL1A2), no hot spots for the mutations were associated with particular clinical phenotypes. Eight patients that were studied here, diagnosed with OI by clinical standards, are from the Polish population with no ethnic background indicated. Previously unpublished mutations were found in six out of those eight patients. Genotypes for polymorphisms (Sp1 - rs1800012 and PvuII - rs412777), linked to bone formation and metabolism were determined. Mutations were found in exons 2, 22, 50 and in introns 13 and 51 of the COL1A1 gene. In COL1A2, one mutation was identified in exon 22. Deletion type mutations in COL1A1 that resulted in OI type I had no effect on collagen type I secretion, nor on its intracellular accumulation. Also, a single base substitution in I13 (c.904-9 G>T) was associated with the OI type I. The OI type III was associated with a single base change in I51 of COL1A1, possibly causing an exon skipping. Also, a missense mutation in COL1A2 changing Gly→Cys in the central part of the triple helical domain of the collagen type I molecule caused OI type III. It affected secretion of the heterotrimeric form of procollagen type I. However, no intracellular accumulation of procollagen chains could be detected. Mutation in COL1A2 affected its incorporation into procollagen type I. The results obtained shall help in genetic counseling of OI patients and provide a rational support for making informed, life important decisions by them and their families

Characteristic of cells isolated from human Abdominal Aortic Aneurysm samples cultured in vitro

Abstract Background: This study aimed to standardize cell culture methods for major cell types isolated from three layers of human AAA. We also aimed to determine cell types in each layer of each AAA segment and compare them with cell types in layers of control, unchanged segments. Material and methods We divided AAAs into three segments along the AAA and control segments flanking the aneurysm. Isolated cells following expansion were analyzed by flow cytometry, immunochemistry and microscopic methods. Fluorochrome-conjugated antibodies were used to detect the three major cell types (endothelial cells, smooth muscle cells, and fibroblasts) in each layer of every AAA segment. Results: Culture of cells from the three AAA segments was successfully established in 21% of patients. In all of the layers, only a small proportion of cells showed layer- specific markers of cell types. The majority of cells from every layer were positive for CD90, which is considered specific marker of fibroblasts in the aorta. Conclusions: We describe methodology for isolation of cells, their culture conditions, and phenotypic characterization for AAA. The wall of AAA loses its specific types of cells in all of the layers compared with the normal abdominal aortic wall

The Forty-Sixth Euro Congress on Drug Synthesis and Analysis: Snapshot

The 46th EuroCongress on Drug Synthesis and Analysis (ECDSA-2017) was arranged within the celebration of the 65th Anniversary of the Faculty of Pharmacy at Comenius University in Bratislava, Slovakia from 5-8 September 2017 to get together specialists in medicinal chemistry, organic synthesis, pharmaceutical analysis, screening of bioactive compounds, pharmacology and drug formulations; promote the exchange of scientific results, methods and ideas; and encourage cooperation between researchers from all over the world. The topic of the conference, Drug Synthesis and Analysis, meant that the symposium welcomed all pharmacists and/or researchers (chemists, analysts, biologists) and students interested in scientific work dealing with investigations of biologically active compounds as potential drugs. The authors of this manuscript were plenary speakers and other participants of the symposium and members of their research teams. The following summary highlights the major points/topics of the meeting

The care of a pregnant patient with type I diabetes.

Wstęp:Cukrzyca typu I, to jedna z coraz częściej występujących chorób przewlekłych wśród populacji kobiet w wieku prokreacyjnym. Pomimo dobrego poznania choroby i postępu medycyny, choroba ta może stanowić podłoże wielu powikłań w ciąży zarówno dla kobiety, jak i płodu. Ważne jest, aby w opiece nad ciężarną w ciąży powikłanej cukrzycą brał udział multidyscyplinarny zespół składający się z wielu specjalistów w dziedzinie medycyny oraz rodzina pacjentki, a model opieki i edukacja powinny być dobrane indywidualnie do danej kobiety.Cel pracy: Celem pracy było przedstawienie aktualnej wiedzy odnośnie prowadzenia ciąży powikłanej cukrzycą typu I oraz przedstawienie procesu pielęgnowania ciężarnejze współistniejącą cukrzycą I typu.Materiał i metody: W pracy wykorzystano metodę studium przypadku. Analizowano sytuację zdrowotną ciężarnej z rozpoznaniem cukrzycy typu I. Zastosowano następujące techniki badawcze: obserwacja, wywiad, analiza dokumentacji medycznej. Narzędziami badawczymi były: karta historii choroby, karta ciąży, indywidualna karta zleceń lekarskich jednorazowych oraz wielorazowych, karta gorączkowa, karta pomiaru glukozy we krwi, skala NRS, glukometr.Podsumowanie: Odpowiednio dobrana indywidualnie opieka holistyczna nad ciężarnymi w ciąży powikłanej cukrzycą typu I zapewnia utrzymanie dobrostanu zarówno matki, jak i dziecka. Monitorowanie przebiegu ciąży, edukacja oraz przestrzeganie zdrowego stylu życia i insulinoterapii zmniejsza ryzyko wystąpienia powikłań u dziecka oraz u matki zarówno w ciąży jak i w połogu.Wnioski: Po wdrożeniu indywidualnego planu opieki, pacjentka została otoczona szczególną opieką zarówno w sferze fizycznej, jak i psychicznej. Działania edukacyjne zastosowane wobec kobiety przygotowały ją do samoobserwacji i samoopieki nie tylko w okresie ciąży ale i w okresie poporodowym.Introduction: Type I diabetes is one of the most common chronic diseases among women of reproductive age. Despite the good knowledge of the disease and the progress of medicine, this disease can be the cause of many complications in pregnancy not only for women but also for the fetus.It is important that a multidisciplinary team consisting of many specialists and the patient's family participate in the care of a pregnant woman with diabetes, and the model of care and education should be selected individually.Objective:The aim of the study was to present the current knowledge on the management of pregnancy complicated by type I diabetes and to present the process of nursing a pregnant woman.Methods:The case study method was used in the work. The health situation of a pregnant woman diagnosed with type I diabetes was analyzed.The following research techniques were used: observation, interview, analysis of medical records. The research tools were: medical history card, pregnancy card, individual medical order card, single-use and reusable, fever card, blood glucose measurement card, nursing reports and courses, NRS scale, glucometer.Summary:Appropriately selected individually holistic care for pregnant women in pregnancy complicated by type 1 diabetes ensures the well-being of both mother and child. Monitoring the course of pregnancy, education and adherence to a healthy lifestyle and insulin therapy reduce the risk of complications during pregnancy and after childbirth.Conclusions:After implementing an individual care plan, the patient was provided with special care both in the physical and mental spheres. She was prepared for self-care through education related to the postpartum period

Can Whole-Body Cryotherapy with Subsequent Kinesiotherapy Procedures in Closed Type Cryogenic Chamber Improve BASDAI, BASFI, and Some Spine Mobility Parameters and Decrease Pain Intensity in Patients with Ankylosing Spondylitis?

The present study investigated whether whole-body cryotherapy (WBC) procedures could potentially have more beneficial effects on index of BASDAI and BASFI, pain intensity, and spine mobility parameters: Ott test, modified Schober test, chest expansion in ankylosing spondylitis (AS) patients, than kinesiotherapy procedures used separately. AS patients were exposed to a cycle of WBC procedures lasting 3 minutes a day, with a subsequent 60 minutes of kinesiotherapy or 60 minutes of kinesiotherapy only, for 10 consecutive days excluding weekend. After the completion of the cycle of WBC procedures with subsequent kinesiotherapy in the AS patients, BASDAI index decreased about 40% in comparison with the input value, whereas in the group of patients who received only kinesiotherapy it decreased only about 15% in comparison with the input value. After the completion of the treatment in the WBC group, BASFI index decreased about 30% in comparison with the input value, whereas in the kinesiotherapy group it only decreased about 16% in comparison with the input value. The important conclusion was that, in WBC group with subsequent kinesiotherapy, we observed on average about twice better results than in the group treated only by kinesiotherapy

Processes of apoptosis and cell proliferation in uterine myomas originating from reproductive and perimenopausal women

We studied uterine myomas originating from females of reproductive age and from females of perimenopausal age. Uterine myomas represent benign tumors of the myometrium, and they develop frequently in women of reproductive age. The frequency of uterine myomas increases with age until women reach the menopause. The study included patients with a myomatous uterus, in the reproductive age or peri-menopausal age, independently evaluating small and large myomas. Myometrial alterations in their direct vicinity were evaluated independently of the myomas. The study included evaluation of immunolocalization of two index proteins which participate in myoma cells growth control: Ki-67 nuclear antigen and caspase 3. In women of reproductive age, both in small and large myomas, elevated immunostaining of Ki-67 was noted in parallel to low levels of caspase 3 staining, which indicated the ongoing process of proliferation. In women of peri-menopausal age with small or large myomas, no Ki-67 immunostaining was detected, while staining of caspase 3 manifested low levels. Proliferation in reproductive age women myomas is higher than in the peri-menopausal age. (<i>Folia Histochemica et Cytobiologica 2011; Vol. 49, No. 3, pp. 398–404</i>

Mutations in COL1A1 and COL1A2 Genes Associated with Osteogenesis Imperfecta (OI) Types I or III.

Although over 85% of osteogenesis imperfecta (OI) cases are associated with mutations in the procollagen type I genes (COL1A1 or COL1A2), no hot spots for the mutations were associated with particular clinical phenotypes. Eight patients that were studied here, diagnosed with OI by clinical standards, are from the Polish population with no ethnic background indicated. Previously unpublished mutations were found in six out of those eight patients. Genotypes for polymorphisms (Sp1 - rs1800012 and PvuII - rs412777), linked to bone formation and metabolism were determined. Mutations were found in exons 2, 22, 50 and in introns 13 and 51 of the COL1A1 gene. In COL1A2, one mutation was identified in exon 22. Deletion type mutations in COL1A1 that resulted in OI type I had no effect on collagen type I secretion, nor on its intracellular accumulation. Also, a single base substitution in I13 (c.904-9 G>T) was associated with the OI type I. The OI type III was associated with a single base change in I51 of COL1A1, possibly causing an exon skipping. Also, a missense mutation in COL1A2 changing Gly→Cys in the central part of the triple helical domain of the collagen type I molecule caused OI type III. It affected secretion of the heterotrimeric form of procollagen type I. However, no intracellular accumulation of procollagen chains could be detected. Mutation in COL1A2 affected its incorporation into procollagen type I. The results obtained shall help in genetic counseling of OI patients and provide a rational support for making informed, life important decisions by them and their families