3,149 research outputs found
Disorder-induced freezing of dynamical spin fluctuations in underdoped cuprates
We study the dynamical spin susceptibility of a correlated d-wave
superconductor (dSC) in the presence of disorder, using an unrestricted
Hartree-Fock approach. This model provides a concrete realization of the notion
that disorder slows down spin fluctuations, which eventually "freeze out". The
evolution of disorder-induced spectral weight transfer agrees qualitatively
with experimental observations on underdoped cuprate superconductors. For
sufficiently large disorder concentrations, static spin density wave (SDW)
order is created when droplets of magnetism nucleated by impurities overlap. We
also study the disordered stripe state coexisting with a dSC and compare its
magnetic fluctuation spectrum to that of the disorder-generated SDW phase.Comment: 5 pages, 4 figure
Q-band electron nuclear double resonance (ENDOR) and X-band EPR of the sulfobetaine 12 heat-treated cytochrome c oxidase complex
Heat treatment of the bovine cytochrome c oxidase complex in the zwitterionic detergent sulfobetaine 12 (SB-12) results in loss of subunit III and the appearance of a type II copper center as characterized by electron paramagnetic resonance (EPR) spectroscopy. Previous authors (Nilsson, T., Copeland, R. A., Smith, P. A., and Chan, S. I. (1988) Biochemistry 27, 8254-8260) have interpreted this type II copper center as a modified version of the CuA site. By using electron nuclear double resonance spectroscopy, it is found that the CuA proton and nitrogen resonances remain present in the SB-12 heat-treated enzyme and that three new nitrogen resonances appear having hyperfine coupling constants consistent with histidine ligation. These hyperfine coupling constants correlate well with those recently found for the CuB histidines from the cytochrome aa3-600 quinol oxidase from Bacillus subtilis (Fann, Y. C., Ahmed, I., Blackburn, N. J., Boswell, J. S., Verkhovskaya, M. L., Hoffman, B. M., and Wikström, M. (1995) Biochemistry 34, 10245-10255). In addition, the total EPR-detectable copper concentration per enzyme molecule approximately doubles upon SB-12 heat treatment. Finally, the observed type II copper EPR spectrum is virtually indistinguishable from the EPR spectrum of CuB of the as-isolated cytochrome bo3 complex from Escherichia coli. These data indicate that the type II copper species that appears results from a breaking of the strong antiferromagnetic coupling of the heme a3-CuB binuclear center
Horizontal Structures of Velocity and Temperature Boundary Layers in 2D Numerical Turbulent Rayleigh-B\'{e}nard Convection
We investigate the structures of the near-plate velocity and temperature
profiles at different horizontal positions along the conducting bottom (and
top) plate of a Rayleigh-B\'{e}nard convection cell, using two-dimensional (2D)
numerical data obtained at the Rayleigh number Ra=10^8 and the Prandtl number
Pr=4.4 of an Oberbeck-Boussinesq flow with constant material parameters. The
results show that most of the time, and for both velocity and temperature, the
instantaneous profiles scaled by the dynamical frame method [Q. Zhou and K.-Q.
Xia, Phys. Rev. Lett. 104, 104301 (2010) agree well with the classical
Prandtl-Blasius laminar boundary layer (BL) profiles. Therefore, when averaging
in the dynamical reference frames, which fluctuate with the respective
instantaneous kinematic and thermal BL thicknesses, the obtained mean velocity
and temperature profiles are also of Prandtl-Blasius type for nearly all
horizontal positions. We further show that in certain situations the
traditional definitions based on the time-averaged profiles can lead to
unphysical BL thicknesses, while the dynamical method also in such cases can
provide a well-defined BL thickness for both the kinematic and the thermal BLs.Comment: 16 pages, 16 figure
Optimal Taylor-Couette flow: direct numerical simulations
We numerically simulate turbulent Taylor-Couette flow for independently
rotating inner and outer cylinders, focusing on the analogy with turbulent
Rayleigh-B\'enard flow. Reynolds numbers of and
of the inner and outer cylinders, respectively, are
reached, corresponding to Taylor numbers Ta up to . Effective scaling
laws for the torque and other system responses are found. Recent experiments
with the Twente turbulent Taylor-Couette () setup and with a similar
facility in Maryland at very high Reynolds numbers have revealed an optimum
transport at a certain non-zero rotation rate ratio
of about . For large enough in the numerically
accessible range we also find such an optimum transport at non-zero
counter-rotation. The position of this maximum is found to shift with the
driving, reaching a maximum of for . An
explanation for this shift is elucidated, consistent with the experimental
result that becomes approximately independent of the driving strength
for large enough Reynolds numbers. We furthermore numerically calculate the
angular velocity profiles and visualize the different flow structures for the
various regimes. By writing the equations in a frame co-rotating with the outer
cylinder a link is found between the local angular velocity profiles and the
global transport quantities.Comment: Under consideration for publication in JFM, 31 pages, 25 figure
Atividade biológica das toxinas do Bt, Cry 1A(b) e Cry 1F em Spodoptera frugiperda (Smith) (Lepidoptera: noctuidae).
Atualmente, há duas diferentes toxinas do Bacillus thuringiensis (Bt) disponíveis em milho transgênico. Entretanto, há poucas informações sobre a toxicidade dessas proteínas puras Cry 1A(b) e Cry 1F para a lagarta-do-cartucho do milho (LCM), Spodoptera frugiperda, (Smith) usando o método de tratamento superficial da dieta. Os bioensaios foram conduzidos, usando um gradiente de concentração para as duas toxinas. Cada tratamento foi infestado artificialmente com larvas da LCM recém-eclodidas e mantidas sob temperatura constante e no escuro. Dez dias após a infestação, o número de larvas sobreviventes e a inibição de crescimento baseado na biomassa foram avaliados. Os dados de mortalidade foram submetidos à análise de "Probit". As CL50 estimadas para Cry1A(b) e Cry1F foram, respectivamente, 689,81 ng/cm2 e 36,46 ng/cm2. A CL50 foi suficiente para inibir o acúmulo de biomassa das larvas em 91,61% e 89,81% para Cry1Ab e Cry1F, respectivamente. As larvas que sobreviveram por dez dias nas dietas tratadas com o Cry 1A(b) foram transferidas para dieta não tratadas e observadas até a emergência dos adultos. Das larvas sobreviventes, 62,8% recuperaram seu desenvolvimento, acumulando a biomassa de uma pupa normal. Portanto, a atividade biológica da toxina do Bt vai além da simples toxicidade, incluindo uma significativa inibição de alimentação. Esse fato tem implicações importantes para o desenvolvimento de estratégias para o manejo da resistência
Optimal Taylor-Couette flow: Radius ratio dependence
Taylor-Couette flow with independently rotating inner (i) and outer (o)
cylinders is explored numerically and experimentally to determine the effects
of the radius ratio {\eta} on the system response. Numerical simulations reach
Reynolds numbers of up to Re_i=9.5 x 10^3 and Re_o=5x10^3, corresponding to
Taylor numbers of up to Ta=10^8 for four different radius ratios {\eta}=r_i/r_o
between 0.5 and 0.909. The experiments, performed in the Twente Turbulent
Taylor-Couette (T^3C) setup, reach Reynolds numbers of up to Re_i=2x10^6$ and
Re_o=1.5x10^6, corresponding to Ta=5x10^{12} for {\eta}=0.714-0.909. Effective
scaling laws for the torque J^{\omega}(Ta) are found, which for sufficiently
large driving Ta are independent of the radius ratio {\eta}. As previously
reported for {\eta}=0.714, optimum transport at a non-zero Rossby number
Ro=r_i|{\omega}_i-{\omega}_o|/[2(r_o-r_i){\omega}_o] is found in both
experiments and numerics. Ro_opt is found to depend on the radius ratio and the
driving of the system. At a driving in the range between {Ta\sim3\cdot10^8} and
{Ta\sim10^{10}}, Ro_opt saturates to an asymptotic {\eta}-dependent value.
Theoretical predictions for the asymptotic value of Ro_{opt} are compared to
the experimental results, and found to differ notably. Furthermore, the local
angular velocity profiles from experiments and numerics are compared, and a
link between a flat bulk profile and optimum transport for all radius ratios is
reported.Comment: Submitted to JFM, 28 pages, 17 figure
Prandtl-Blasius temperature and velocity boundary layer profiles in turbulent Rayleigh-B\'{e}nard convection
The shape of velocity and temperature profiles near the horizontal conducting
plates in turbulent Rayleigh-B\'{e}nard convection are studied numerically and
experimentally over the Rayleigh number range and the Prandtl number range . The results show that both the temperature and velocity
profiles well agree with the classical Prandtl-Blasius laminar boundary-layer
profiles, if they are re-sampled in the respective dynamical reference frames
that fluctuate with the instantaneous thermal and velocity boundary-layer
thicknesses.Comment: 10 pages, 6 figure
Keberadaan Mikroba Pada Bakso Ikan Asap Cair, Yang Dikemas Dalam Retortable Pouch, Dipasteurisasi Dan Disimpan Pada Temperatur Ruang
A research has been done to assess the presence of microbes and the shelf life of fish balls dipped in liquid smoke, packed in retortable pouch, pasteurized, and stored at room temperature. The method used in this research is an experimental method that revealed the facts based on existing problems through hypothesis testing. Parameters assessed are Total Plate Count (TPC), total Salmonella, total coliforms and E. coli, total vibrio, water content, and pH. Fish balls were pasteurized in 85ºC and stored in room temperature for 0, 3, 6, and 9 days, and were pasteurized at 100°C and storage in room temperature for 0, 9, 18, and 27 days. The results shown that all pathogen were negative, but based on Indonesian standard (SNI 01-7266-1-2006) especially TPC value, fish balls were packaged in retortable pouch and without pasteurized is no longer acceptable for consumption after 3 days of storage, and fish balls were packaged in retortable pouch and pasteurized at 85ºC no longer acceptable for consumed after 6 days of storage. While the fish balls are packaged in retortable pouch and pasteurized at 100°C is no longer acceptable for consumed after 18 days of storage. Keyword: Retortable Pouch, Pasteurization, Pathogens. Penelitian ini bertujuan untuk mengetahui keberadaan mikroba dan daya awet bakso ikan yang direndam dalam asap cair dan dikemas dalam Retortable pouch, dipasteurisasi, dan disimpan pada temperatur ruang. Metode yang digunakan dalam penelitian ini adalah metode eksperimen untuk menguji hipotesa. Parameter yang diamati yaitu Angka Lempeng Total (ALT), Salmonella, total koliform dan E.coli, total vibrio, analisa kadar air, dan nilai pH. Bakso ikan asap cair yang dipasteurisasi pada 85ºC, disimpan pada temperatur ruang selama 0, 3, 6, dan 9 hari, dan yang dipasteurisasi pada 100ºC, disimpan pada temperatur ruang selama 0,9,18, dan 27 hari. Hasil penelitian menunjukkan bahwa bakso ikan yang dikemas dalam Retortable pouch, tanpa dipasteurisasi sudah tidak layak lagi untuk dikonsumsi setelah penyimpanan 3 hari, dan bakso ikan yang dikemas dalam Retortable pouch dan dipasteurisasi pada 85ºC sudah tidak layak lagi untuk dikonsumsi setelah penyimpanan 6 hari. Bakso ikan yang dikemas dalam Retortable pouch, dan dipasteurisasi pada 100ºC nanti tidak layak lagi dikonsumsi setelah penyimpanan 18 hari
Selection of the fall armyworm Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae) for survival on Cry 1A (b) Bt toxin.
Plantas transgênicas que expressam toxinas da bactéria Bacillus thuringiensis (Bt) representam um importante avanço para o controle de lepidópteros-pragas de milho. Híbridos de milho Bt expressando a toxina Cry1A(b) têm mostrado significativo nível de resistência à lagarta-do-cartucho (LCM), Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae), embora larvas dessa espécie tenham sido observadas em campo e em laboratório sobrevivendo nesses híbridos. Resistência de S. frugiperda também tem sido registrada para diferentes inseticidas. Em bioensaios, seleção de insetos sobreviventes mostrou um aumento do nível de tolerância à toxina do Bt Cry1A(b) em populações da lagarta-do-cartucho, após quatro gerações, indicando que essa tolerância é herdada. Palavras-chave: Insecta, resistência, plantas transgênicas
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