3 research outputs found
Organic source of vanadium preparation and physical-chemical characteristic
Antidiabetic properties of vanadium are known more than 100 years, however the researches of specific therapeutic usage of vanadium were conducted only in the last two decades. Along with, the organic vanadium compounds are more harmless in comparison with inorganic vanadium salts. Thus, the development of method of obtaining the organic source of vanadium with high bioavailability is prospective field. Aim of the work was to obtain and provide the physical-chemical characterization of vanadium complex with enzymatic hydrolysate of soy protein isolate (SPI), obtained by one-stage enzymatic hydrolysis. Material and methods. The complex was obtained at room temperature: 10% water solu-tion of SPI was mixed with 25% solution of vanadium salt (VOS04'xxH20) in ratio 10:1 (in dry matter). The reaction was kept during 1 hat constant mixing with pH kept at 7.0-7.1 with 1.0 MNaOH. The concentration of vanadium was determined in dry product by means of inductively coupled plasma mass-spectrometry. The chromatograms of SPI and V-SPI were obtained by means of size-exclusion high-pressure liquid chromatography, and then were integrated by weight method in the range of free till full column volume. Results and discussion. The obtained complex of vanadium with SPI enzymatic hydrolysate (V-SPI) was water-soluble and contained 15.8 mg of vanadium per gram of product dry weight. Analysis of the molecular weight distribution of the peptide fractions of the original SPI enzymatic hydrolysate and the V-SPI complex showed that more than 87% of the vanadium complex was in peptide fractions with molecular weights more than 4.1 kD, including more than 75% of vanadium contained in fractions with molecular weights from 14.6 to 4.1 kD. Conclusion. The experimental evaluation in vivo will be the next stage of this research. The complex bioavailability and its effects on carbohydrate and lipid metabolism of Wistar rats with obesity will be evaluated. Β© 2019 Nutritec. All rights reserved
ΠΡΠ³Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΠΉ ΠΈΡΡΠΎΡΠ½ΠΈΠΊ Π²Π°Π½Π°Π΄ΠΈΡ. ΠΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΠΈ ΡΠΈΠ·ΠΈΠΊΠΎ-Ρ ΠΈΠΌΠΈΡΠ΅ΡΠΊΠ°Ρ Ρ Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ°
Antidiabetic properties of vanadium are known more than 100 years, however the researches of specific therapeutic usage of vanadium were conducted only in the last two decades. Along with, the organic vanadium compounds are more harmless in comparison with inorganic vanadium salts. Thus, the development of method of obtaining the organic source of vanadium with high bioavailability is prospective field. Aim of the work was to obtain and provide the physical-chemical characterization of vanadium complex with enzymatic hydrolysate of soy protein isolate (SPI), obtained by one-stage enzymatic hydrolysis. Materialand methods. The complex was obtained at room temperature: 10% water solution of SPI was mixed with 25% solution of vanadium salt (VOSO4ΓΡ
H2O) in ratio 10:1 (in dry matter). The reaction was kept during 1 h at constant mixing with pH kept at 7.0- 7.1 with 1.0 M NaOH. The concentration of vanadium was determined in dry product by means of inductively coupled plasma mass-spectrometry. The chromatograms of SPI and V-SPI were obtained by means of size-exclusion high-pressure liquid chromatography, and then were integrated by weight method in the range of free till full column volume. Results and discussion. The obtained complex of vanadium with SPI enzymatic hydrolysate (V-SPI) was water-soluble and contained 15.8 mg of vanadium per gram of product dry weight. Analysis of the molecular weight distribution of the peptide fractions of the original SPI enzymatic hydrolysate and the V-SPI complex showed that more than 87% of the vanadium complex was in peptide fractions with molecular weights more than 4.1 kD, including more than 75% of vanadium contained in fractions with molecular weights from 14.6 to 4.1 kD. Conclusion. The experimental evaluation in vivo will be the next stage of this research. The complex bioavailability and its effects on carbohydrate and lipid metabolism of Wistar rats with obesity will be evaluated.ΠΠ½ΡΠΈΠ΄ΠΈΠ°Π±Π΅ΡΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠ²ΠΎΠΉΡΡΠ²Π° ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ Π²Π°Π½Π°Π΄ΠΈΡ ΠΈΠ·Π²Π΅ΡΡΠ½Ρ ΡΠΆΠ΅ Π±ΠΎΠ»Π΅Π΅ 100 Π»Π΅Ρ, ΠΎΠ΄Π½Π°ΠΊΠΎ ΡΠΎΠ»ΡΠΊΠΎ Π² ΠΏΠΎΡΠ»Π΅Π΄Π½ΠΈΠ΅ 20 Π»Π΅Ρ ΠΏΡΠΎΠ²Π΅Π΄Π΅Π½Ρ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ ΠΏΠΎ ΠΊΠΎΠ½ΠΊΡΠ΅ΡΠ½ΠΎΠΌΡ ΡΠ΅ΡΠ°ΠΏΠ΅Π²ΡΠΈΡΠ΅ΡΠΊΠΎΠΌΡ ΠΈΡΠΏΠΎΠ»ΡΠ·ΠΎΠ²Π°Π½ΠΈΡ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΠΉ Π²Π°Π½Π°Π΄ΠΈΡ. ΠΡΠΈ ΡΡΠΎΠΌ ΠΎΡΠ³Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΠ΅ ΡΠΎΠ΅Π΄ΠΈΠ½Π΅Π½ΠΈΡ Π²Π°Π½Π°Π΄ΠΈΡ Π±ΠΎΠ»Π΅Π΅ Π±Π΅Π·ΠΎΠΏΠ°ΡΠ½Ρ ΠΏΠΎ ΡΡΠ°Π²Π½Π΅Π½ΠΈΡ Ρ Π½Π΅ΠΎΡΠ³Π°Π½ΠΈΡΠ΅ΡΠΊΠΈΠΌΠΈ ΡΠΎΠ»ΡΠΌΠΈ ΡΡΠΎΠ³ΠΎ ΠΌΠΈΠΊΡΠΎΡΠ»Π΅ΠΌΠ΅Π½ΡΠ°. Π‘ΠΎΠΎΡΠ²Π΅ΡΡΡΠ²Π΅Π½Π½ΠΎ ΠΏΠ΅ΡΡΠΏΠ΅ΠΊΡΠΈΠ²Π½ΠΎΠΉ ΠΏΡΠ΅Π΄ΡΡΠ°Π²Π»ΡΠ΅ΡΡΡ ΡΠ°Π· ΡΠ°Π±ΠΎΡΠΊΠ° ΠΌΠ΅ΡΠΎΠ΄Π° ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΡ ΠΎΡΠ³Π°Π½ΠΈΡΠ΅ΡΠΊΠΎΠ³ΠΎ ΠΈΡΡΠΎΡΠ½ΠΈΠΊΠ° Π²Π°Π½Π°Π΄ΠΈΡ, ΠΎΠ±Π»Π°Π΄Π°ΡΡΠ΅Π³ΠΎ Π²ΡΡΠΎΠΊΠΎΠΉ Π±ΠΈΠΎΠ΄ΠΎΡΡΡΠΏΠ½ΠΎΡΡΡΡ. Π¦Π΅Π»ΡΡ Π΄Π°Π½Π½ΠΎΠΉ ΡΠ°Π±ΠΎΡΡ Π±ΡΠ»ΠΎ ΠΏΠΎΠ»ΡΡΠ΅Π½ΠΈΠ΅ ΠΈ ΡΠΈΠ·ΠΈΠΊΠΎ-Ρ
ΠΈΠΌΠΈΡΠ΅ΡΠΊΠ°Ρ Ρ
Π°ΡΠ°ΠΊΡΠ΅ΡΠΈΡΡΠΈΠΊΠ° ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ° ΡΠ΅ΡΡΡΠ΅Ρ
Π²Π°Π»Π΅Π½ΡΠ½ΠΎΠ³ΠΎ Π²Π°Π½Π°Π΄ΠΈΡ Ρ ΡΠ΅ΡΠΌΠ΅Π½ΡΠ°ΡΠΈΠ²Π½ΡΠΌ Π³ΠΈΠ΄ΡΠΎΠ»ΠΈΠ·Π°ΡΠΎΠΌ ΠΈΠ·ΠΎΠ»ΡΡΠ° ΡΠΎΠ΅Π²ΠΎΠ³ΠΎ Π±Π΅Π»ΠΊΠ° (Π€ΠΠΠΠ‘), ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΠΎΠ³ΠΎ ΠΏΠΎ ΠΎΠ΄Π½ΠΎΡΡΠ°Π΄ΠΈΠΉΠ½ΠΎΠΉ ΡΡ
Π΅ΠΌΠ΅ ΡΠ΅ΡΠΌΠ΅Π½ΡΠΎΠ»ΠΈΠ·Π° Π² ΠΏΠΎΠ»ΡΠΏΡΠΎΠΌΡΡΠ»Π΅Π½Π½ΡΡ
ΠΌΠ°ΡΡΡΠ°Π±Π°Ρ
. ΠΠ°ΡΠ΅ΡΠΈΠ°Π» ΠΈ ΠΌΠ΅ΡΠΎΠ΄Ρ. Π Π΅Π°ΠΊΡΠΈΡ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠΎΠΎΠ±ΡΠ°Π·ΠΎΠ²Π°Π½ΠΈΡ ΠΏΡΠΎΠ²ΠΎΠ΄ΠΈΠ»ΠΈ ΠΏΡΠΈ ΠΊΠΎΠΌΠ½Π°ΡΠ½ΠΎΠΉ ΡΠ΅ΠΌΠΏΠ΅ΡΠ°ΡΡΡΠ΅: ΠΊ 10% Π²ΠΎΠ΄Π½ΠΎΠΌΡ ΡΠ°ΡΡΠ²ΠΎΡΡ Π€ΠΠΠΠ‘ Π΄ΠΎΠ±Π°Π²Π»ΡΠ»ΠΈ ΠΏΡΠΈ ΠΏΠ΅ΡΠ΅ΠΌΠ΅ΡΠΈΠ²Π°Π½ΠΈΠΈ 25% ΡΠ°ΡΡΠ²ΠΎΡ ΡΠΎΠ»ΠΈ Π²Π°Π½Π°Π΄ΠΈΡ (VOSO4ΓΡ
H2O) Π² ΡΠΎΠΎΡΠ½ΠΎΡΠ΅Π½ΠΈΠΈ ΠΏΠΎ ΡΡΡ
ΠΈΠΌ Π²Π΅ΡΠ΅ΡΡΠ²Π°ΠΌ 10:1. Π Π΅Π°ΠΊΡΠΈΡ Π²Π΅Π»ΠΈ Π² ΡΠ΅ΡΠ΅Π½ΠΈΠ΅ 1 Ρ ΠΏΡΠΈ ΠΏΠΎΡΡΠΎΡΠ½Π½ΠΎΠΌ ΠΏΠ΅ΡΠ΅ΠΌΠ΅ΡΠΈΠ²Π°Π½ΠΈΠΈ Ρ ΠΏΠΎΠ΄Π΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ΠΌ ΡΠ ΡΠ΅Π°ΠΊΡΠΈΠΎΠ½Π½ΠΎΠΉ ΡΠΌΠ΅ΡΠΈ 7,0-7,1 1,0 Π NaOH. Π‘ΠΎΠ΄Π΅ΡΠΆΠ°Π½ΠΈΠ΅ Π²Π°Π½Π°Π΄ΠΈΡ ΠΎΠΏΡΠ΅Π΄Π΅Π»ΡΠ»ΠΈ Π² ΡΡΡ
ΠΎΠΌ ΠΏΡΠΎΠ΄ΡΠΊΡΠ΅ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΠΌΠ°ΡΡ-ΡΠΏΠ΅ΠΊΡΡΠΎΠΌΠ΅ΡΡΠΈΠΈ Ρ ΠΈΠ½Π΄ΡΠΊΡΠΈΠ²Π½ΠΎ ΡΠ²ΡΠ·Π°Π½Π½ΠΎΠΉ ΠΏΠ»Π°Π·ΠΌΠΎΠΉ. Π₯ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΠΌΠΌΡ Π€ΠΠΠΠ‘ ΠΈ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ° Π²Π°Π½Π°Π΄ΠΈΡ Ρ Π€ΠΠΠΠ‘ (V-Π€ΠΠΠΠ‘) ΠΏΠΎΠ»ΡΡΠ°Π»ΠΈ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ ΡΠΊΡΠΊΠ»ΡΠ·ΠΈΠΎΠ½Π½ΠΎΠΉ ΠΆΠΈΠ΄ΠΊΠΎΡΡΠ½ΠΎΠΉ Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΠΈ Π²ΡΡΠΎΠΊΠΎΠ³ΠΎ Π΄Π°Π²Π»Π΅Π½ΠΈΡ, ΠΊΠΎΡΠΎΡΡΠ΅ Π·Π°ΡΠ΅ΠΌ ΠΈΠ½ΡΠ΅Π³ΡΠΈΡΠΎΠ²Π°Π»ΠΈ Π²Π΅ΡΠΎΠ²ΡΠΌ ΠΌΠ΅ΡΠΎΠ΄ΠΎΠΌ Π² Π΄ΠΈΠ°ΠΏΠ°Π·ΠΎΠ½Π΅ ΠΎΡ ΡΠ²ΠΎΠ±ΠΎΠ΄Π½ΠΎΠ³ΠΎ Π΄ΠΎ ΠΏΠΎΠ»Π½ΠΎΠ³ΠΎ ΠΎΠ±ΡΠ΅ΠΌΠ° Ρ
ΡΠΎΠΌΠ°ΡΠΎΠ³ΡΠ°ΡΠΈΡΠ΅ΡΠΊΠΎΠΉ ΠΊΠΎΠ»ΠΎΠ½ΠΊΠΈ. Π Π΅Π·ΡΠ»ΡΡΠ°ΡΡ ΠΈ ΠΎΠ±ΡΡΠΆΠ΄Π΅Π½ΠΈΠ΅. ΠΠΎΠ»ΡΡΠ΅Π½Π½ΡΠΉ Π² Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΡΡ
ΡΡΠ»ΠΎΠ²ΠΈΡΡ
ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡ V-Π€ΠΠΠΠ‘ Ρ
ΠΎΡΠΎΡΠΎ ΡΠ°ΡΡΠ²ΠΎΡΡΠ»ΡΡ Π² Π²ΠΎΠ΄Π΅ ΠΈ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π» 15,8 ΠΌΠ³ Π²Π°Π½Π°Π΄ΠΈΡ Π½Π° 1 Π³ ΡΡΡ
ΠΎΠ³ΠΎ ΠΏΡΠΎΠ΄ΡΠΊΡΠ°. ΠΠ½Π°Π»ΠΈΠ· ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΠΎ-ΠΌΠ°ΡΡΠΎΠ²ΠΎΠ³ΠΎ ΡΠ°ΡΠΏΡΠ΅Π΄Π΅Π»Π΅Π½ΠΈΡ ΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΡΡ
ΡΡΠ°ΠΊΡΠΈΠΉ ΠΈΡΡ
ΠΎΠ΄Π½ΠΎΠ³ΠΎ Π€ΠΠΠΠ‘ ΠΈ ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ° V-Π€ΠΠΠΠ‘ ΠΏΠΎΠΊΠ°Π·Π°Π», ΡΡΠΎ Π±ΠΎΠ»Π΅Π΅ 87% Π²Π°Π½Π°Π΄ΠΈΡ ΠΊΠΎΠΌΠΏ Π»Π΅ΠΊΡΠ° Π½Π°Ρ
ΠΎΠ΄ΠΈΠ»ΠΎΡΡ Π² ΡΠΎΡΡΠ°Π²Π΅ ΠΏΠ΅ΠΏΡΠΈΠ΄Π½ΡΡ
ΡΡΠ°ΠΊΡΠΈΠΉ Ρ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠΌΠΈ ΠΌΠ°ΡΡΠ°ΠΌΠΈ Π±ΠΎΠ»Π΅Π΅ 4,1 ΠΊΠΠ°, Π² ΡΠΎΠΌ ΡΠΈΡΠ»Π΅ Π±ΠΎΠ»Π΅Π΅ 75% Π²Π°Π½Π°Π΄ΠΈΡ ΡΠΎΠ΄Π΅ΡΠΆΠ°Π»ΠΎΡΡ Π²ΠΎ ΡΡΠ°ΠΊΡΠΈΡΡ
Ρ ΠΌΠΎΠ»Π΅ΠΊΡΠ»ΡΡΠ½ΡΠΌΠΈ ΠΌΠ°ΡΡΠ°ΠΌΠΈ ΠΎΡ 14,6 Π΄ΠΎ 4,1 ΠΊΠΠ°. ΠΠ°ΠΊΠ»ΡΡΠ΅Π½ΠΈΠ΅. ΠΡΠ΅Π΄ΠΌΠ΅ΡΠΎΠΌ Π΄Π°Π»ΡΠ½Π΅ΠΉΡΠ΅Π³ΠΎ ΠΈΡΡΠ»Π΅Π΄ΠΎΠ²Π°Π½ΠΈΡ Π°Π²ΡΠΎΡΠΎΠ² Π±ΡΠ΄Π΅Ρ ΡΠΊΡΠΏΠ΅ΡΠΈΠΌΠ΅Π½ΡΠ°Π»ΡΠ½Π°Ρ ΠΎΡΠ΅Π½ΠΊΠ° in vivo Π±ΠΈΠΎΠ΄ΠΎΡΡΡΠΏΠ½ΠΎΡΡΠΈ ΠΈ Π²Π»ΠΈΡΠ½ΠΈΡ Π½Π° ΡΠ³Π»Π΅Π²ΠΎΠ΄Π½ΡΠΉ ΠΈ Π»ΠΈΠΏΠΈΠ΄Π½ΡΠΉ ΠΎΠ±ΠΌΠ΅Π½ Ρ Π»Π°Π±ΠΎΡΠ°ΡΠΎΡΠ½ΡΡ
ΠΆΠΈΠ²ΠΎΡΠ½ΡΡ
Ρ ΠΌΠΎΠ΄Π΅Π»ΠΈΡΡΠ΅ΠΌΡΠΌ ΠΎΠΆΠΈΡΠ΅Π½ΠΈΠ΅ΠΌ ΠΏΠΎΠ»ΡΡΠ΅Π½Π½ΠΎΠ³ΠΎ Π½Π°ΠΌΠΈ Π½ΠΎΠ²ΠΎΠ³ΠΎ ΠΈΡΡΠΎΡΠ½ΠΈΠΊΠ° ΠΎΡΠ³Π°Π½ΠΈΡΠ΅ΡΠΊΠΎΠΉ ΡΠΎΡΠΌΡ Π²Π°Π½Π°Π΄ΠΈΡ-ΠΊΠΎΠΌΠΏΠ»Π΅ΠΊΡΠ°